Cereals high in amylose content (AC) and resistant starch (RS) offer potential health benefits. Previous studies using chemical mutagenesis or RNA interference have demonstrated that branching enzyme (SBE) plays a major role determining the fine structure physical properties of starch. However, it remains challenge to control commercial lines. Here, we use CRISPR/Cas9 technology generate targeted SBEI SBEIIb rice. The frequencies obtained homozygous bi-allelic mutant lines with indels T0...

Foods high in amylose content and resistant starch (RS) offer great potential to improve human health lower the risk of serious noninfectious diseases. Common wheat (Triticum aestivum L.) is a major staple food crop globally. However, RS contents grains modern varieties are low. Here, we report generation high-amylose through targeted mutagenesis TaSBEIIa winter cv Zhengmai 7698 (ZM) spring Bobwhite by CRISPR/Cas9, respectively. We generated series transgene-free mutant lines either with...

<title>Abstract</title> Virus-induced genome editing (VIGE) technologies have been developed to address the limitations of plant editing, which heavily relies on genetic transformation and regeneration. However, application VIGE in plants is hampered by challenge posed size commonly used gene-editing nucleases, Cas9 Cas12a. To this challenge, our study explores potential inteins VIGE, internal protein elements known for mediating post-translational splicing self-excising from precursor...

Virus-induced genome editing (VIGE) technologies have been developed to address the limitations plant editing, which heavily relies on genetic transformation and regeneration. However, application of VIGE in plants is hampered by challenge posed size commonly used gene nucleases, Cas9 Cas12a. To overcome this challenge, we employed intein-mediated protein splicing divide SaCas9 transcript into two segments (Split-v1) three (Split-v3). The Split-v1 system demonstrated efficiencies transgenic...

Summary The Cas12j‐8 nuclease, derived from the type V CRISPR system, is approximately half size of Cas9 and recognizes a 5′‐TTN‐3′ protospacer adjacent motif sequence, thus potentially having broad application in genome editing for crop improvement. However, its efficiency remains low plants. In this study, we rationally engineered both crRNA nuclease. markedly improved When combined, they exhibited robust activity soybean rice, enabling target sites that were previously uneditable....

Beef cattle are often fed high-concentrate diet (HCD) to achieve high growth rate. However, HCD feeding is strongly associated with metabolic disorders. Mild acid treatment of grains in 1% hydrochloric (HA) followed by neutralization sodium bicarbonate (SB) might modify rumen fermentation patterns and microbiota, thereby decreasing the negative effects HCD. This study was thus aimed investigate corn HA subsequent SB on inflammatory response performance beef Eighteen were randomly allocated...

Summary Two full‐length c DNA s (762 bp) of the DRA gene from yak and C hinese yakow were isolated analysed to identify structural functional variations. The sequences for in ( B ogr ‐DRA ) × o LA ‐ essentially identical those cattle (99%) buffalo (97%). Except two substitutions amino acids comprising domain signal peptide SP yak, additional residues highly conserved across species investigated. Peptide‐binding site PBS ogr‐ was reserved α1 among all lack mutation is consistent with...

摘要: 成熟期穗发芽严重影响小麦产量和品质。Vp1是调节胚发育, 促进胚成熟和休眠的重要转录因子, 对小麦种子休眠和穗发芽抗性具有重要作用。本研究分离了普通小麦B基因组Vp1基因的启动子, 生物信息学预测结果表明, 其含有9个脱落酸响应元件ABRE、2个DREB和6个MYB干旱响应元件、3个赤霉素响应元件GARE、1个水杨酸响应元件TCA-E、2个茉莉酸甲酯响应元件TGACG-motif、4个SKn-1和1个RYREPE胚乳特异表达元件。采用5′端缺失的方法, 构建了系列含Vp1启动子不同区段融合GUS报告基因的瞬时表达载体和植物表达载体。通过基因枪转化小麦愈伤组织, 瞬时表达结果显示, Vp1启动子在无诱导的情况下不能启动GUS基因表达, 在低温、ABA、GA、PEG和NaCl诱导后可以启动GUS基因表达, 表现诱导表达特性, 且其诱导表达强度随启动子缺失片段长度变短而减弱。利用Gateway方法成功构建了6个启动子各缺失片段类型的植物表达载体, 并通过农杆菌介导转化四倍体小麦Stewart,...

Pre-harvest sprouting (PHS) is one of important factors affecting yield and quality wheat (Triticum aestivum L.).Generally, red-grained has higher PHS resistance than white wheat.Transcription factor Tamyb10 a strong candidate for R-1 gene the expression its homologues determines seed coat color.In this study, we attempted to analyze relationship between haplotype using amplification result specific markers germination index (GI) evaluated in two locations over years.In 119 red-grain Chinese...

<title>Abstract</title> Virus-induced genome editing (VIGE) technologies have been developed to address the limitations of plant editing, which heavily relies on genetic transformation and regeneration. However, application VIGE in plants is hampered by challenge posed size commonly used gene-editing nucleases, Cas9 Cas12a. To this challenge, our study explores potential inteins VIGE, internal protein elements known for mediating post-translational splicing self-excising from precursor...

Objective: To investigate the epidemiological characteristics and transmission chain of a family clustering COVID-19 cases caused by severe acute respiratory 2019-nCoV Delta variant in Changping district Beijing. Methods: Epidemiological investigation was conducted big data were used to reveal exposure history cases. Close contacts screened according results, human environmental samples collected for nucleic acid tests. Positive analyzed gene sequencing. Results: On November 1, 2021, total 5...

Objective: To investigate the source and transmission chain of a cold-chain product associated COVID-19 epidemic caused by 2019-nCoV Delta variant in Beijing. Methods: Epidemiological investigation were used to verify exposure points cases. Close contacts traced from points, human environmental samples collected for nucleic acid tests. Positive analyzed gene sequencing. Results: A total 112 cases reported January 18 February 6, 2022 Except 1 case was uncertain, there epidemiological links...

The complete coding sequences of three Black-boned sheep (Ovis aries) genes DFP, ITM2B and PQLC1 were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) according to conserved sequence information cattle or other mammals known highly homologous ESTs. DFP gene encodes a protein 213 amino acids which contains putative DNA/pantothenate metabolism flavoprotein domain has high homology with proteins four species: (99%), dog (91%), human (88%) zebrafish (53%). 266 that...

untranslated region and one synonymous substitution in coding were identified.These data suggest that high immunodiversity native

Intraorganismal genetic heterogeneity (IGH) exists when an individual organism harbors more than one genotype among its cells. In general, intercellular DNA diversity occurs at a very low frequency and cannot be directly detected by sequencing from bulk tissue. this study, based on Sanger high-throughput sequencing, different species, organs, segments single cell were employed to characterize nucleotide mutations in Leymus chinensis. The results demonstrated that 1) the nuclear showed...

Objective: To investigate the infection sources and transmission chains of three outbreaks caused by 2019-nCoV Omicron variant possibly spread through cross-border logistics in Beijing. Methods: Epidemiological investigation big data were used to identify exposure points cases. Close contacts traced from points, cases' environmental samples collected for nucleic acid tests. Positive analyzed gene sequencing. Results: The causing 3 Beijing January April, 2022 belonged BA.1, BA.1.1 BA.2....