A5002435489- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Hearing Loss and Rehabilitation
- Hearing, Cochlea, Tinnitus, Genetics
- Noise Effects and Management
- Optical Coherence Tomography Applications
- Photoreceptor and optogenetics research
- Advanced Manufacturing and Logistics Optimization
- Optimization and Packing Problems
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Radiomics and Machine Learning in Medical Imaging
- Scientific Measurement and Uncertainty Evaluation
- Advanced Radiotherapy Techniques
- Advanced Memory and Neural Computing
- Maritime Navigation and Safety
- Advanced Statistical Process Monitoring
- Vehicle Routing Optimization Methods
- Zebrafish Biomedical Research Applications
- Molecular Communication and Nanonetworks
- Lung Cancer Diagnosis and Treatment
- Kidney Stones and Urolithiasis Treatments
- Neuroscience and Music Perception
- Photoacoustic and Ultrasonic Imaging
- Neuroscience and Neuropharmacology Research
Suzhou University of Science and Technology
2021-2025
University of Science and Technology of China
2021-2025
Chinese Academy of Sciences
2019-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Suzhou Institute of Biomedical Engineering and Technology
2019-2025
Ministry of Education of the People's Republic of China
2024
Capital Medical University
2017-2024
Google (United States)
2024
Beijing Tongren Hospital
2017-2024
Lancaster University
2023-2024
Abstract The sensory responses of cortical neuronal populations following training have been extensively studied. However, the spike firing properties individual neurons remain unknown. Here, we combined two-photon Ca 2+ imaging and single-cell electrophysiology in awake behaving mice auditory associative training. We find a sparse set (~5%) layer 2/3 primary cortex, each which reliably exhibits high-rate prolonged burst to trained sound. Such bursts are largely absent cortex untrained mice....
We study interactive video calls between two users, where at least one of the users is connected over a cellular network. It known that links present highly-varying network bandwidth and packet delays. If sending rate call exceeds available bandwidth, frames may be excessively delayed, destroying interactivity call. In this paper, we Rebera, cross-layer design proactive congestion control, encoding adaptation, to maximize transmission while keeping one-way frame delays sufficiently low....
Abstract Reconstructing axonal projections of single neurons at the whole-brain level is currently a converging goal neuroscience community that fundamental for understanding logic information flow in brain. Thousands from different brain regions have recently been morphologically reconstructed, but corresponding physiological functional features these reconstructed are unclear. By combining two-photon Ca 2+ imaging with targeted single-cell plasmid electroporation, we reconstruct brain-wide...
Roving histopathological imaging in the navigation-confirmed tumor negative margin has not been achieved during neurosurgery procedures. This study aims to explore diagnostic values of a novel intraoperative handheld endomicroscopy DiveScope (DS) for ex vivo glioma diagnosis and roving scan evaluate its safety. prospective pilot was conducted at [university/hospital name] from October 2021 2023, enrolled patients scheduled resection surgery. The DS scanning were compared with frozen section...
Two-photon laser scanning microscopy has been extensively applied to study in vivo neuronal activity at cellular and subcellular resolutions mammalian brains. However, the extent of such studies is typically confined a single functional region brain. Here, we demonstrate novel technique, termed multiarea two-photon real-time explorer (MATRIEX), that allows user target multiple brain regions distributed within zone up 12 mm diameter, each with field view (FOV) ~200 μm thus performing Ca2+...
Neocortical layer 6 (L6) is less understood than other more superficial layers, largely owing to limitations of performing high-resolution investigations in vivo. Here, we show that labeling with the Challenge Virus Standard (CVS) rabies virus strain enables high-quality imaging L6 neurons by conventional two-photon microscopes. CVS injection into medial geniculate body can selectively label auditory cortex. Only three days after injection, dendrites and cell bodies could be imaged across...
Studying chemosensory processing desires precise chemical cue presentation, behavioral response monitoring, and large-scale neuronal activity recording. Here we present Fish-on-Chips, a set of optofluidic tools for highly-controlled delivery while simultaneously imaging outputs whole-brain activities at cellular resolution in larval zebrafish. These include fluidics-based swimming arena an integrated microfluidics-light sheet fluorescence microscopy (µfluidics-LSFM) system, both which...
Abstract Recent developments in real‐time, vivo micro‐imaging have allowed for the visualization of tissue pathological changes, facilitating rapid diagnosis. However, miniaturization, magnification, field view, and image stabilization remain challenging factors to reconcile. A key issue this technology is ensuring it user friendly surgeons, enabling them use device manually obtain instantaneous information necessary surgical decision‐making. This descriptive study introduces a handheld,...
Astrocytic Ca2+ transients are essential for astrocyte integration into neural circuits. These primarily sequestered in subcellular domains, including primary branches, branchlets and leaflets, endfeet. In previous studies, it suggests that aging causes functional defects astrocytes. Until now, was unclear whether how affects astrocytic at domains. this study, we combined a genetically encoded sensor (GCaMP6f) vivo two-photon imaging to determine changes within domains during brain aging. We...
Background: The development of auditory and speech perception ability children with hearing loss is affected by many factors after they undergo cochlear implantation (CI). Age at CI (CI age) appears to play an important role among these factors. This study aimed evaluate the explore impact age on pre-lingual deafness present before 3 years age. Methods: Two hundred seventy-eight (176 boys 102 girls) were included in this study, ranged from 6 36 months (mean age, 19 months). Categorical...
Abstract The sensory neocortex has been suggested to be a substrate for long-term memory storage, yet which exact single cells could specific candidates underlying such storage remained neither known nor visible over century. Here, using combination of day-by-day two-photon Ca 2+ imaging and targeted single-cell loose-patch recording in an auditory associative learning paradigm with composite sounds male mice, we reveal sparsely distributed neurons layer 2/3 cortex emerged step-wise from...
The studying of rapid neuronal signaling across large spatial scales in intact, living brains requires both high temporal resolution and versatility the measurement device.
Two-photon laser scanning microscopy, originally developed since 1990s 1 , has been widely applied for biomedical research in recent decades, particularly popular among neuroscientists studying neural functions vivo 2 . However, it is typically restricted to one imaging area that orthogonal the optical axis. Here, we demonstrate a novel multi-axis conjugation method enables two-photon at single-cell resolution simultaneously multiple areas different depths, each of which could have view...
In order to solve the problem of image real-time processing and correction for high-speed endoscopic swept-source optical coherence tomography (SS-OCT), we highly optimize a computer-unified device architecture-based platform use field-programmable gate array summarize application experience.We Half-Sync/Half-Asyn mode memory in build high-throughput data thread pool CPU. We asynchronous streaming architecture multiplex multiple threads at high speed accelerate processing. At same time,...
Purpose: The purpose of this study was to evaluate the auditory performance and speech perception 104 children with isolated large vestibular aqueduct syndrome (LVAS) 523 no inner ear malformation (IEM) for 5 years after cochlear implantation, in order explore whether LVAS can affect long-term hearing rehabilitation deaf implantation. Method: A cohort established consisting 627 who underwent implantation at Beijing Tongren Hospital from 1999 2016. were examined 0, 6, 12, 24, 36, 48, 60...