A5100723707- Urinary Bladder and Prostate Research
- Circadian rhythm and melatonin
- Neuroscience of respiration and sleep
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Memory and Neural Mechanisms
- Pelvic floor disorders treatments
- Advanced Fluorescence Microscopy Techniques
- Urinary Tract Infections Management
- Cell Image Analysis Techniques
- Kidney Stones and Urolithiasis Treatments
- Gestational Diabetes Research and Management
- Sarcoidosis and Beryllium Toxicity Research
- Urological Disorders and Treatments
- Neonatal Health and Biochemistry
- Adenosine and Purinergic Signaling
- COVID-19 Clinical Research Studies
- Sleep and related disorders
- Thermoregulation and physiological responses
- Heme Oxygenase-1 and Carbon Monoxide
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Vagus Nerve Stimulation Research
- Cytokine Signaling Pathways and Interactions
Army Medical University
2008-2024
Southwest Hospital
2008-2024
Guangxi University
2019-2024
Third Affiliated Hospital of Zhengzhou University
2024
New York University Press
2023
State Council of the People's Republic of China
2020-2023
Cambridge University Press
2023
UK Dementia Research Institute
2023
University College London
2023
Suzhou Institute of Biomedical Engineering and Technology
2023
A close view of the paraventricular thalamus The is a relay station connecting brainstem and hypothalamic signals that represent internal states with limbic forebrain performs associative functions in emotional contexts. Zhu et al. found thalamic neurons multiple salient features sensory stimuli, including reward, aversiveness, novelty, surprise. nucleus thus provides context-dependent salience encoding. gates information contributes to sleep-wake cycle through its interactions cerebral...
Abstract The sensory responses of cortical neuronal populations following training have been extensively studied. However, the spike firing properties individual neurons remain unknown. Here, we combined two-photon Ca 2+ imaging and single-cell electrophysiology in awake behaving mice auditory associative training. We find a sparse set (~5%) layer 2/3 primary cortex, each which reliably exhibits high-rate prolonged burst to trained sound. Such bursts are largely absent cortex untrained mice....
Background: Down-regulation of suppressor cytokine signaling 3 (SOCS3) inhibits prostate cancer (PCa) cell growth. Liver X receptors (LXRs) agonists have been recently introduced for PCa treatment. We postulated that LXR may inhibit the carcinogenesis via SOCS3 pathway. Methods: LNCaP cells were cultured and transfected with small-interfering RNA (SOCS3-siRNA) control (control-siRNA). Then treated activator (GW3965). The expressions related transcript factors, e.g. transcription (STAT3),...
Two-photon laser scanning microscopy has been extensively applied to study in vivo neuronal activity at cellular and subcellular resolutions mammalian brains. However, the extent of such studies is typically confined a single functional region brain. Here, we demonstrate novel technique, termed multiarea two-photon real-time explorer (MATRIEX), that allows user target multiple brain regions distributed within zone up 12 mm diameter, each with field view (FOV) ~200 μm thus performing Ca2+...
The ventrolateral periaqueductal gray (VLPAG) is thought to be the main PAG column for bladder control. neurons (especially VLPAG neurons) and in pontine micturition center (PMC) innervating detrusor have anatomical functional synaptic connections. prevailing viewpoint on neural control of that receive information decision void made by upstream brain regions, consequently activate PMC through their direct projections initiate urination reflex. However, exact location PMC-projecting neurons,...
Locomotion involves complex neural activity throughout different cortical and subcortical networks. The primary motor cortex (M1) receives a variety of projections from brain regions is responsible for executing movements. visual (V1) external stimuli plays an important role in guiding locomotion. Understanding how exactly the M1 V1 are involved locomotion requires recording activities these areas freely moving animals. Here, we used optical fiber-based method real-time monitoring neuronal...
In vivo two-photon Ca2+ imaging is a powerful tool for recording neuronal activities during perceptual tasks and has been increasingly applied to behaving animals acute or chronic experiments. However, the auditory cortex not easily accessible because of abundant temporal muscles, arteries around ears their lateral locations. Here, we report protocol in head-fixed mice. By using custom-made head fixation apparatus head-rotated procedure, achieved combination with targeted cell-attached...
Understanding the complex neural mechanisms controlling urinary bladder activity is an extremely important topic in both neuroscience and urology. Simultaneously recording of related brain regions will largely advance this field. However, such approach has long been restricted to anesthetized animals, whose function urodynamic properties are affected by anesthetics. In our recent report, we found that it feasible record pressure (cystometry) cortical neuron simultaneously freely moving mice....
To explore the value of optical coherence tomography (OCT) imaging system in evaluating cervical lesions vivo.
Neurogenic lower urinary tract dysfunction (NLUTD) is a frequent consequence of spinal cord injury (SCI), leading to symptoms that significantly impact quality life. Although many life-saving techniques are available, current treatment strategies for managing NLUTD still exhibit limitations and drawbacks. Here, we introduce new electrical neuromodulation strategy involving stimulation the major pelvic ganglion (MPG) initiate bladder contraction, in conjunction with innovative programmable...
Background: To date, large amounts of epidemiological and case study data have been available for the Coronavirus Disease 2019 (COVID-19), which suggested that mortality was related to not just respiratory complications. Here, we specifically analyzed kidney functions in COVID-19 patients their relations mortality. Methods: In this multi-centered, retrospective, observational study, included 193 adult with laboratory-confirmed from 2 hospitals Wuhan, 1 hospital Huangshi (Hubei province, 83...
This study aims to determine the association of pregnancy cholesterol metabolism markers with gestational diabetes mellitus (GDM) risk. We performed a nested case–control in Tongji Birth Cohort. GDM was diagnosed according 75 g 2 h oral glucose tolerance test (OGTT) at 24–28 weeks. Nine were detected using gas chromatography-mass spectrometry. Conditional logistic regression models conducted. A total 444 pregnant women matched 1:2 ratio. The cholestanolTC and β-sitosterolTC absorption...
Two-photon laser scanning microscopy, originally developed since 1990s 1 , has been widely applied for biomedical research in recent decades, particularly popular among neuroscientists studying neural functions vivo 2 . However, it is typically restricted to one imaging area that orthogonal the optical axis. Here, we demonstrate a novel multi-axis conjugation method enables two-photon at single-cell resolution simultaneously multiple areas different depths, each of which could have view...
Micturition serves an essential physiological function that allows the body to eliminate metabolic wastes and maintain water-electrolyte balance. The urine spot assay (VSA), as a simple economical assay, has been widely used in study of micturition behavior rodents. However, traditional VSA method relies on manual judgment, introduces subjective errors, faces difficulty obtaining appearance time each spot, struggles with quantitative analysis overlapping spots. To address these challenges,...
<title>Abstract</title> Neurogenic lower urinary tract dysfunction (NLUTD) is a frequent consequence of spinal cord injury (SCI), leading to symptoms that significantly impact quality life. However, existing treatment strategies for managing NLUTD exhibit limitations and drawbacks. The demand novel, effective approach restore bladder function re-establish urination control. In this study, we introduce new electrical neuromodulation strategy involving stimulation the major pelvic ganglion...
Lower urinary tract symptoms (LUTS) are common in postmenopausal women. These often linked to decreased estrogen levels following menopause. This study investigated the relationship between levels, alterations bladder tissue structure, function, and incidence of frequency. An age-appropriate bilateral ovariectomized mouse model (OVX) was developed simulate conditions deficiency. Mice were divided into three groups: a sham-operated control group, OVX, an estradiol-treated group. The...