A5076909940- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Science Education and Pedagogy
- Educational Strategies and Epistemologies
- Education and Critical Thinking Development
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Adipose Tissue and Metabolism
- Innovative Teaching Methods
- Adipokines, Inflammation, and Metabolic Diseases
- Immune cells in cancer
- Cell Adhesion Molecules Research
- Blood disorders and treatments
- Inflammatory mediators and NSAID effects
- Inflammasome and immune disorders
- Evaluation of Teaching Practices
- Muscle Physiology and Disorders
- Hormonal Regulation and Hypertension
- Educator Training and Historical Pedagogy
- Atherosclerosis and Cardiovascular Diseases
- Online and Blended Learning
- Diet and metabolism studies
- Infective Endocarditis Diagnosis and Management
- Vitamin D Research Studies
- Liver Disease Diagnosis and Treatment
- Phytase and its Applications
Nanyang Technological University
2019-2025
University of Massachusetts Amherst
2023-2024
Tan Tock Seng Hospital
2023-2024
BC Cancer Agency
2022
Harvard University
2009-2020
Boston Children's Hospital
2013-2020
Xiamen University Malaysia
2020
Monash University Malaysia
2019
University of Hong Kong
2007-2017
University of Malaya
2017
Deep vein thrombosis and pulmonary embolism are major health problems associated with high mortality. Recently, DNA-based neutrophil extracellular traps (NETs) resulting from the release of decondensed chromatin, were found to be part thrombus scaffold promote coagulation. However, significance nuclear decondensation NET generation in is largely unknown. To address this, we adopted a stenosis model deep analyzed venous thrombi peptidylarginine deiminase 4 (PAD4)-deficient mice that cannot...
Significance Neutrophils are white blood cells specialized as the first line of host defense in immune system. One way they protect organisms is through NETosis, which expel their DNA to form a web-like trap that ensnares pathogens and promotes clotting. However, NETs also mediate sterile inflammation, causing damage body. We used high-resolution live-cell microscopy characterize timing dynamic cellular events leading NETosis human mouse neutrophils neutrophil-like cell line. discovered...
Neutrophils play a major role in cancer biology and both pro- antitumoral functions of tumor-infiltrating neutrophils have been described. We shown that tumors, by releasing G-CSF into the bloodstream, prime circulating to form neutrophil extracellular traps (NETs) we detected presence NETs within tumor microenvironment. Here, report, using PAD4-deficient mice with defect chromatin decondensation NET formation, priming toward NETosis favors growth. Interestingly, model does not release which...
Abstract This study sought to identify prominent features of the nature science (NOS) embedded in authentic scientific inquiry. Thirteen well‐established scientists from different parts world, working experimental or theoretical research, both traditional fields such as astrophysics and rapidly growing research molecular biology, completed an open‐ended questionnaire about NOS participated in‐depth interviews. The descriptions their practices provide a somewhat striking contrast image...
Although fatty acids enhance preadipocyte differentiation in the presence of adequate hormone cocktails, little is known regarding their effects absence these hormones. We have now shown that palmitate, a common long-chain saturated acid, induced apoptosis both mouse 3T3-L1 and rat primary preadipocytes grown normal serum-containing medium. Treatment with palmitate multiple endoplasmic reticulum (ER) stress responses, evidenced by increased protein content CHOP GRP78 splicing XBP-1 mRNA, as...
AimsThe present study investigated whether or not calcitriol, an active form of vitamin D, protects against renovascular dysfunction in hypertension and, if so, such protection alters the expression key proteins involved that dysfunction.
Testosterone administration increases hemoglobin levels and has been used to treat anemia of chronic disease. Erythrocytosis is the most frequent adverse event associated with testosterone therapy hypogonadal men, especially older men. However, mechanisms by which remain unknown. in male female mice was a greater increase hematocrit, reticulocyte count, concentration, serum iron transferrin saturation than placebo. downregulated hepatic hepcidin mRNA expression, upregulated renal...
Hypertension and vascular dysfunction result in the increased release of endothelium-derived contracting factors (EDCFs), whose identity is poorly defined. We tested hypothesis that endothelial cyclooxygenase (COX)-2 can generate EDCFs identified possible EDCF candidate. Changes isometric tension aortae young aged hamsters were recorded on myograph. Real-time changes intracellular calcium concentrations ([Ca 2+ ] i ) native aortic cells measured by imaging. Endothelium-dependent contractions...
ABSTRACT Peptidylarginine deiminase 4 (PAD4) is a nuclear citrullinating enzyme that critically involved in the release of decondensed chromatin from neutrophils as neutrophil extracellular traps (NETs). NETs, together with fibrin, are implicated host defense against pathogens; however, formation NETs (NETosis) has injurious effects may outweigh their protective role. For example, PAD4 activity produces citrullinated neoantigens promote autoimmune diseases, such rheumatoid arthritis, to...
Abstract This research study sought to identify prominent features of the nature science (NOS) embedded in authentic scientific inquiry. Fourteen well‐established scientists from different parts world, working experimental or theoretical research, both traditional fields such as astrophysics and rapidly growing molecular biology, participated informants study. The descriptions their practices revealed eight categories NOS features. In an earlier paper ours, we have reported four under two...
Development and Validation of a New Simple Liquid Chromatographic Method for Determination Coumarin its 7-Hydroxy Metabolite as Marker Cytochrome P450 2A6 in RatsSundeep Bath, Wael Ebied, Hytham Ahmed, Fawzy Elbarbry
Enterococcus faecalis is an opportunistic pathogen that frequently co-isolated with other microbes in wound infections. While E. can subvert the host immune response and promote survival of via interbacterial synergy, little known about impact faecalis-mediated suppression on co-infecting microbes. We hypothesized attenuate neutrophil-mediated responses mixed-species infection to species. found neutrophils control phagocytosis, ROS production, degranulation azurophilic granules, but it does...
Written in response to criticism of our work by Fouad Abd-El-Khalick, this position paper reaffirms and reinforces about the need broaden enrich scope nature science (NOS) oriented curricula exposing students voices practising scientists. While Abd-El-Khalick's motivation for promoting so-called consensus view NOS is rooted issues assessment (or 'benchmarking', as he calls it), we argue that major reason teaching its contribution what Shen civic cultural scientific literacy. We are critical...
Men with prostate cancer who receive androgen deprivation therapy show profound skeletal muscle loss. We hypothesized that the deficiency activates not only ubiquitin-proteasome systems but also autophagy and affects key aspects of molecular cross talk between protein synthesis degradation. Here, 2-month-old male mice were castrated treated either testosterone (T) propionate or vehicle for 7 days (short term) 43 (long term), without hydroxyflutamide. Castrated showed rapid atrophy levator...
Heme oxygenase-1 (HO-1) exerts vasoprotective effects. Such benefit in diabetic vasculopathy, however, remains unclear. We hypothesize that bilirubin mediates HO-1-induced vascular benefits diabetes. Diabetic db/db mice were treated with hemin (HO-1 inducer) for 2 weeks, and aortas isolated functional molecular assays. Nitric oxide (NO) production was measured cultured endothelial cells. Hemin treatment augmented endothelium-dependent relaxations (EDRs) elevated Akt NO synthase (eNOS)...