A5077394217- Advanced MRI Techniques and Applications
- Protease and Inhibitor Mechanisms
- S100 Proteins and Annexins
- Atherosclerosis and Cardiovascular Diseases
- Cerebrovascular and Carotid Artery Diseases
- Nanoparticle-Based Drug Delivery
- Liver Disease Diagnosis and Treatment
- Neurotransmitter Receptor Influence on Behavior
- Metabolomics and Mass Spectrometry Studies
- Optical Imaging and Spectroscopy Techniques
- Nanocluster Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Functional Brain Connectivity Studies
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Medical Imaging Techniques and Applications
- Connexins and lens biology
- NMR spectroscopy and applications
- Bone health and osteoporosis research
- Advanced Neuroimaging Techniques and Applications
- Single-cell and spatial transcriptomics
- Pain Mechanisms and Treatments
- Spectroscopy Techniques in Biomedical and Chemical Research
- High Altitude and Hypoxia
- Renal function and acid-base balance
University College London
1998-2021
National Hospital for Neurology and Neurosurgery
2017-2021
Imperial College London
2011-2017
NIHR Imperial Biomedical Research Centre
2014-2016
St Mary's Hospital
2016
Medical Research Council
2011-2014
Hammersmith Hospital
2011
University of Calgary
2009
The blood–brain barrier (BBB), a critical guardian of communication between the periphery and brain, is frequently compromised in neurological diseases such as multiple sclerosis (MS), resulting inappropriate passage molecules leukocytes into brain. Here we show that glucocorticoid anti-inflammatory messenger annexin A1 (ANXA1) expressed brain microvascular endothelial cells, where it regulates BBB integrity. In particular, ANXA1 −/− mice exhibit significantly increased permeability result...
Abstract MRI offers high spatial resolution with excellent tissue penetration but it has limited sensitivity and the commonly administered contrast agents lack specificity. In this study, two sets of iron oxide nanoparticles (IONPs) were synthesized that designed to selectively undergo copper‐free click conjugation upon sensing matrix metalloproteinase (MMP) enzymes, thereby leading a self‐assembled superparamagnetic nanocluster network T 2 signal enhancement properties. For purpose, IONPs...
Research ethics committee approval was obtained for this study, and written informed consent from all participants. The purpose to prospectively evaluate the feasibility of breath-hold multiecho in- out-of-phase magnetic resonance (MR) imaging simultaneous lipid quantification T2* measurement. A spoiled gradient-echo sequence with seven echo times alternately in phase out used at 3.0 T. Imaging performed a phantom, five healthy volunteers (all men; mean age, 37 years), obese individuals...
Preclinical models for musculoskeletal disorders are critical understanding the pathogenesis of bone and joint in humans development effective therapies. The assessment these primarily relies on morphological analysis which remains time consuming costly, requiring large numbers animals to be tested through different stages disease. implementation preclinical imaging represents a keystone refinement animal allowing longitudinal studies enabling powerful, non-invasive clinically translatable...
Förster resonance energy transfer (FRET) is a powerful biological tool for reading out cell signaling processes. In vivo use of FRET challenging because the scattering properties bulk tissue. By combining diffuse fluorescence tomography with lifetime imaging (FLIM), implemented using wide-field time-gated detection excited by ultrashort laser pulses in tomographic system and applying inverse algorithms, we can reconstruct three dimensional spatial localization quantum efficiency lifetime. We...
Summary Background Brain change can occur in primary biliary cholangitis ( PBC ), potentially as a result of cholestatic and/or inflammatory processes. This is linked to systemic symptoms fatigue and cognitive impairment. Aim To identify whether brain occurs early . If the develops progressive, it may explain difficulty treating these symptoms. Methods Early disease was explored 13 patients with newly diagnosed biopsy‐proven precirrhotic using magnetisation transfer, diffusion‐weighted...
Formyl Peptide Receptors (FPRs) are vital in the host inflammatory response, playing an important regulatory role multiple diseases. A Gd(III) DOTA conjugate of cFLFLFK has been synthesised which targets and visualises FPR1 upon leukocytes response via magnetic resonance imaging for first time.
As magnetic resonance imaging (MRI) contrast agents, T1 Gd3+ chelates are generally the preferred option for radiologists over T2 iron oxide nanoparticles. The main reason popularity of agents is easier interpretation T1-weighted MR images. However, chemical versatility nanoparticulate platforms makes them ideal candidates next generation targeted MRI agents. In this context, we present herein design and preparation a agent based on MnO, which presents enhancement properties as well...
Blood flow generates wall shear stress (WSS) which alters endothelial cell (EC) function. Low WSS promotes vascular inflammation and atherosclerosis whereas high uniform is protective. Ivabradine decreases heart rate leading to altered haemodynamics. Besides its cardio-protective effects, ivabradine protects arteries from via unknown mechanisms. We hypothesised that by increasing reduce inflammation. Hypercholesterolaemic mice were treated with for seven weeks in drinking water or remained...
The pathophysiological basis of neurological decompression sickness and the association between cerebral subcortical white matter (WM) change nonhypoxic hypobaria remain poorly understood. Recent study altitude risk evaluated acute WM responses to intensive hypobaric exposure using brain magnetic resonance imaging.
Dexamphetamine (AMPH) is a psychostimulant drug that used both recreationally and as medication for attention deficit hyperactivity disorder. Preclinical studies have demonstrated repeated exposure to AMPH can induce damage nerve terminals of dopamine (DA) neurons. We here assessed the underlying neurobiological changes in DA system following pre-treated rats with or saline (4 times 5 mg/kg s.c., 2 hours apart), followed by 1-week washout period. then pharmacological MRI (phMRI)...
Abstract MRI offers high spatial resolution with excellent tissue penetration but it has limited sensitivity and the commonly administered contrast agents lack specificity. In this study, two sets of iron oxide nanoparticles (IONPs) were synthesized that designed to selectively undergo copper‐free click conjugation upon sensing matrix metalloproteinase (MMP) enzymes, thereby leading a self‐assembled superparamagnetic nanocluster network T 2 signal enhancement properties. For purpose, IONPs...
<h3>BACKGROUND AND PURPOSE:</h3> Youthful memory performance in older adults may reflect an underlying resilience to the conventional pathways of aging. Subjects having this unusual characteristic have been recently termed "superagers." This study aimed explore significance imaging biomarkers acquired by <sup>1</sup>H-MRS characterize superagers and differentiate them from their normal-aging peers. <h3>MATERIALS METHODS:</h3> Fifty-five patients than 80 years age were screened using a...
We describe a new light transport model, which was applied to three-dimensional lifetime imaging of Förster resonance energy transfer in mice vivo. The model is an approximation the radiative equation and combines diffusion ray optics. This well adopted wide-field time-gated intensity-based data acquisition. Reconstructed image are presented compared with results obtained by using telegraph approximation. approach provides improved recovery absorption scattering parameters while returning...