A5050898490- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Gut microbiota and health
- Pancreatic function and diabetes
- Diet, Metabolism, and Disease
- Gout, Hyperuricemia, Uric Acid
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Regulation of Appetite and Obesity
- Mass Spectrometry Techniques and Applications
- Probiotics and Fermented Foods
- Liver Disease Diagnosis and Treatment
- Pesticide Exposure and Toxicity
- Genetic Associations and Epidemiology
- Diabetes and associated disorders
- Nutritional Studies and Diet
- Immune cells in cancer
- Biochemical Analysis and Sensing Techniques
- Exercise and Physiological Responses
- Traditional Chinese Medicine Studies
- Infant Health and Development
- Inflammasome and immune disorders
- Fibroblast Growth Factor Research
- Bone Metabolism and Diseases
- Abdominal vascular conditions and treatments
Inserm
2017-2025
Université Paris Cité
2017-2025
Sorbonne Paris Cité
2017-2025
Bioscar
2023-2025
Kyoto University
2021-2024
Hôpital Lariboisière
2023-2024
Toxicologie, Pharmacologie et Signalisation Cellulaire
2020-2021
Délégation Paris 5
2019-2021
Assistance Publique – Hôpitaux de Paris
2021
Centre de Recherche des Cordeliers
2017-2020
The dietary methylamines choline, carnitine, and phosphatidylcholine are used by the gut microbiota to produce a range of metabolites, including trimethylamine (TMA). However, little is known about use N-oxide (TMAO) this consortium microbes. A feeding study using deuterated TMAO in C57BL6/J mice demonstrated microbial conversion TMA, with uptake TMA into bloodstream its TMAO. Microbial activity necessary convert was suppressed antibiotic-treated mice, being taken up directly bloodstream. In...
Objective Gut microbial products are involved in regulation of host metabolism. In human and experimental studies, we explored the potential role hippurate, a hepatic phase 2 conjugation product benzoate, as marker mediator metabolic health. Design 271 middle-aged non-diabetic Danish individuals, who were stratified on habitual dietary intake, applied 1 H-nuclear magnetic resonance (NMR) spectroscopy urine samples shotgun-sequencing-based metagenomics gut microbiome to explore links between...
Abstract Aims/hypothesis Drug and surgical-based therapies in type 2 diabetes are associated with altered gut microbiota architecture. Here we investigated the role of microbiome improved glucose homeostasis following bariatric surgery. Methods We carried out analyses gastrectomised (by vertical sleeve gastrectomy [VSG]) rats Goto–Kakizaki (GK) non-obese model spontaneously occurring diabetes, followed by physiological studies GK rat. Results VSG rat led to permanent improvement tolerance...
Exposure to natural metabolites contributes the risk of cardiometabolic diseases (CMDs). Through metabolome profiling, we identify inverse correlation between serum concentrations 4-cresol and type 2 diabetes. The chronic administration non-toxic doses in complementary preclinical models CMD reduces adiposity, glucose intolerance, liver triglycerides, enhances insulin secretion vivo, stimulates islet density size, pancreatic β-cell proliferation, increases vascularization, suggesting...
Abstract Early diagnosis of acute mesenteric ischemia (AMI) remains a clinical challenge, and no biomarker has been consistently validated. We aimed to assess the accuracy three promising circulating biomarkers for diagnosing AMI—citrulline, intestinal fatty acid-binding protein (I-FABP), d -lactate. A cross-sectional diagnostic study enrolled AMI patients admitted stroke center controls with abdominal pain another origin. included 129 patients—50 79 controls. Plasma citrulline...
Abstract Brain regions drive multiple physiological functions through specific gene expression patterns that adapt to environmental influences, drug treatments and disease conditions. To generate a detailed atlas of the brain transcriptome in context diabetes, we carried out RNA sequencing hypothalamus, hippocampus, brainstem striatum Goto-Kakizaki (GK) rat model spontaneous type 2 which was applied identify transcription adaptation improved glycemic control following vertical sleeve...
Cherubism is a rare genetic disorder caused by SH3BP2 mutations. This sterile autoinflammatory disease characterized jaw osteolysis, in which bone tissue replaced multinucleated giant cells containing fibrous tissue. The cherubism mouse model ( Sh3bp2 KI) systemic loss as well inflammatory phenotypes induced and maintained TNFα. IL-1β, produced the NRLP3 inflammasome through recruitment of Caspase-1, involved development disease. We previously reported patient with elevated serum mice also...
Lipoproteins are major players in the development and progression of atherosclerotic plaques leading to coronary stenosis myocardial infarction. Epidemiological, genetic experimental observations have implicated association sphingolipids intermediates sphingolipid synthesis atherosclerosis. We aimed investigate relationships between quantitative changes serum sphingolipids, regulation metabolism lipoproteins (LDL, HDL), endophenotypes artery disease (CAD). carried out untargeted liquid...
Background and Aims Although Hnf1α is crucial for pancreas liver functions, it believed to play a limited functional role intestinal epithelial functions. The aim of this study was assess the consequences abrogating on maintenance adult small Methodology/Principal Findings An knockout mouse model used. Assessment histological abnormalities, crypt cell proliferation, barrier, glucose transport signalling pathways were measured in these animals. Changes global gene expression also analyzed....
Hepatocyte nuclear factor-1α (HNF1α) is a transcription factor expressed in tissues of endoderm origin. Mutations HNF1A are associated with maturity-onset diabetes the young 3 (MODY3). Mice deficient for Hnf1α hyperglycemic, their pancreatic β-cells being defective glucose-sensing insulin secretion. The specific mechanisms involved this defect unclear. Gut hormones control glucose homeostasis. Our objective was to explore whether changes these play role homeostasis absence Hnf1α. An increase...
Abstract Non-alcoholic fatty liver disease (NAFLD) is often associated with obesity and type 2 diabetes. To disentangle etiological relationships between these conditions identify genetically-determined metabolites involved in NAFLD processes, we mapped 1 H nuclear magnetic resonance (NMR) metabolomic disease-related phenotypes a mouse F2 cross derived from strains showing resistance (BALB/c) increased susceptibility (129S6) to diseases. Quantitative trait locus (QTL) analysis based on...
To define renal molecular mechanisms that are affected by permanent hyperglycaemia and might promote phenotypes relevant to diabetic nephropathy, we carried out linkage analysis of genome-wide gene transcription in the kidneys F2 offspring from Goto-Kakizaki (GK) rat model type 2 diabetes normoglycaemic Brown Norway (BN) rats. We mapped 2526 statistically significant expression quantitative trait loci (eQTLs) cross. More than 40% eQTLs close vicinity linked transcripts, underlying possible...
Aortic valve stenosis (AVS) is a prevalent condition among the elderly population that eventually requires aortic replacement. The lack of reliable biomarkers for AVS poses challenge its early diagnosis and application preventive measures. Untargeted gas chromatography mass spectrometry (GC-MS) metabolomics was applied in 46 cases controls to identify plasma urine metabolites underlying risk. Multivariate data analyses were performed on pre-processed (e.g. spectral peak alignment), order...
ABSTRACT BACKGROUND The dietary methylamines choline, carnitine and phosphatidylcholine are used by the gut microbiota to produce a range of metabolites, including trimethylamine (TMA). However, little is known about use N -oxide (TMAO) this consortium microbes. RESULTS A feeding study using deuterated TMAO in C57BL6/J mice demonstrated microbial conversion TMA, with uptake TMA into bloodstream its TMAO. Microbial activity necessary convert was suppressed antibiotic-treated mice, being taken...
Abstract Gut-microbiota derived metabolites are important regulators of host biology and metabolism. To understand the impacts microbial metabolite 4-cresol sulfate (4-CS) on four chronic diseases [type 2 diabetes mellitus, metabolic syndrome (MetS), non-alcoholic fatty liver disease, kidney disease (CKD)], we conducted association analyses plasma 4-CS quantified by liquid chromatography coupled to mass spectrometry (LC–MS) in 3641 participants Nagahama study. Our results validated elevation...
Abstract The global type 2 diabetes epidemic is a major health crisis and there critical need for innovative strategies to fight it. Although the microbiome plays important roles in onset of insulin resistance (IR) low-grade inflammation, microbial compounds regulating these phenomena remain be discovered. Here, we reveal that inhibits central kinase, eliciting immune metabolic benefits. Through series vivo experiments based on choline supplementation, blocking trimethylamine (TMA)...
4-cresol (4-methylphenol, p-cresol) is a xenobiotic substance negatively correlated with type 2 diabetes and associated health improvement in preclinical models of diabetes. We aimed at refining our understanding the physiological role this metabolite identifying potential signalling mechanisms. Functional studies revealed that does not deteriorate insulin sensitivity human primary adipocytes exhibits an additive effect to on mouse C2C12 myoblasts. Experiments isolated islets showed...
<h3>Background:</h3> Inflammation induced by monosodium urate (MSU) and calcium pyrophosphate dihydrate (CPPD) crystals depends on interleukin (IL)-1β activated the NLRP3 inflammasome. The inflammatory response can be modulated diet. <h3>Objectives:</h3> Does intermittent fasting (IF) reduce inflammation MSU CPPD crystals? Can a metabolite mimic effect of IF? <h3>Methods:</h3> Crystal-induced was assessed in air pouch model male mice fed either ad libitum or subjected to two-day IF. measured...
Analysis of the genetic control small metabolites provides powerful information on regulation endpoints genome expression. We carried out untargeted liquid chromatography−high-resolution mass spectrometry in 273 individuals characterized for pathophysiological elements cardiometabolic syndrome. quantified 3013 serum lipidomic features, which we used both genome-wide association studies (GWAS), using a panel over 2.5 M imputed single-nucleotide polymorphisms (SNPs), and metabolome-wide (MWAS)...
ABSTRACT Objective Gut microbial products are involved in type 2 diabetes, obesity and insulin resistance. In particular, hippurate, a hepatic phase conjugation product of benzoate metabolism, has been associated with healthy phenotype. This study aims to identify metagenomic determinants test protective effects hippurate. Design We profiled the urine metabolome by 1 H Nuclear Magnetic Resonance (NMR) spectroscopy derive associations sequences 271 middle-aged Danish individuals dietary...