A5017020905- vaccines and immunoinformatics approaches
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- interferon and immune responses
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Genomics, phytochemicals, and oxidative stress
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- Multiple Myeloma Research and Treatments
- RNA and protein synthesis mechanisms
- Nanoparticles: synthesis and applications
- Cancer-related Molecular Pathways
- T-cell and B-cell Immunology
- Advanced Glycation End Products research
- Machine Learning in Bioinformatics
- Diabetes, Cardiovascular Risks, and Lipoproteins
- HIV Research and Treatment
- Mass Spectrometry Techniques and Applications
- Insect and Arachnid Ecology and Behavior
- Renal and Vascular Pathologies
University of Gdańsk
2019-2025
Italian Institute of Technology
2012-2017
Center for Biomolecular Nanotechnologies
2014
National Chemical Laboratory
2011-2012
Council of Scientific and Industrial Research
2012
Albumin is one of the most abundant plasma proteins and heavily glycated in diabetes. In this study, we have addressed whether variation albumin levels influence glycation HbA1c. The study was performed three systems: (1) streptozotocin (STZ)-induced diabetic mice plasma, (2) clinical (3) vitro plasma. Diabetic samples were categorized as high (DHAP) low (DLAP) on basis their levels. For experiment, two levels, (HAP) (LAP), created by differential depletion albumin. Protein studied using a...
Worldwide efforts are currently trying to produce effective risk assessment models for orally ingested nanoparticles. These tests should provide quantitative information on the bioaccessibility and bioavailability of products biotransformation, such as dissolved ionic species and/or aggregates. In vitro dissolution might be useful nanoparticle assessment, because their potential quantitatively monitor changes specific properties (e.g., dissolution, agglomeration, etc.), which critical...
Based on the knockdown and overexpression experiments, it is accepted that in Tris-glycine SDS-PAGE human NRF2 migrates above 100 kDa, depending percentage of gel. In 8 % gel, monoclonal anti-NRF2 antibodies detect signal as three bands migrating between 130 kDa. Here we used mass spectrometry to identify proteins immunoprecipitated by this range under steady state, upon activator tert-BHQ after translation inhibition with emetine. Our results show commercial epitopes center C-terminus also...
Melanoma is an aggressive cancer with rising incidence and high mortality rates, largely due to chemotherapy resistance molecular dysregulation. Nanotechnology, particularly silver nanoparticles (AgNPs), has emerged as a promising therapeutic avenue because of the nanoparticles' ability induce oxidative stress apoptosis in cells. However, conventional colloidal AgNPs lack selectivity, often causing significant damage healthy In this study, we introduce green synthesis using plant extracts,...
Abstract Tumor antigens can emerge through multiple mechanisms, including translation of noncoding genomic regions. This noncanonical category tumor has recently gained attention; however, our understanding how they recur within and between cancer types is still in its infancy. Therefore, we developed a proteogenomic pipeline based on deep learning de novo mass spectrometry (MS) to enable the discovery MHC class I–associated peptides (ncMAP) from Considering that emergence also involve...
Developments in quantitative proteomics and data-independent acquisition (DIA) methodology is enabling quantification of proteins biological samples. Currently, there are a few reports on DIA mass spectrometry (MS) approaches for proteome analysis formalin-fixed paraffin-embedded (FFPE) tissues. Therefore, to facilitate detection immune- glioblastoma (GBM)-relevant from FFPE patient materials, we established simple precise DIA-MS workflow. We first evaluated different lysis buffers their...
Abstract Robotically assisted proteomics provides insights into the regulation of multiple proteins achieving excellent spatial resolution. However, developing an effective method for spatially resolved quantitative formalin fixed paraffin embedded tissue (FFPE) in accessible and economical manner remains challenging. We introduce non-robotic In-insert FFPE approach, combining glass insert processing with data-independent mass spectrometry (DIA). approach identifies 450 from a 5 µm thick...
Abstract Background The links between the p53/MDM2 pathway and expression of pro-oncogenic immune inhibitory receptors in tumor cells are undefined. In this report, we evaluate whether there is p53 and/or MDM2 dependence two key receptors, CD276 PD-L1. Methods Proximity ligation assays were used to quantify protein-protein interactions situ response Nutlin-3. A panel p53-null melanoma was created using CRISPR-Cas9 guide RNA mediated genetic ablation. Flow cytometric analyses assess impact...
Proteomics of human tissues and isolated cellular subpopulations create new opportunities for therapy monitoring a patients' treatment in the clinic. Important considerations such analysis include recovery adequate amounts protein reproducibility sample collection. In this study we compared several protocols proteomic preparation: i) filter-aided preparation (FASP), ii) in-solution digestion (ISD) iii) pressure-assisted (PCT) method. PCT method is known already decade [1], however it not...
The TAP1-TAP2 complex transports antigenic peptide substrates into the endoplasmic reticulum (ER). In ER, peptides are further processed and loaded on major histocompatibility class (MHC) I molecules by loading (PLC). TAP transporters linked with PLC; a target for cancers viral immune evasion. But mechanisms whereby cancer-derived mutations in or factors targeting PLC, interfere transport only emerging. This study describes that transit of through can take place via two different channels (4...
Proteomic approaches aid in gaining a better understanding of the pathophysiology diabetic complications. In view this, differential protein expression plasma samples was studied by combination proteomic and western blot analyses. Diabetic were categorized based on glycated haemoglobin levels as controlled diabetes (CD; 7-8%), poorly (PCD; >8%) non-diabetic control (ND;<6.4%). Two-dimensional electrophoresis liquid chromatography‑mass spectrometry revealed proteins including upregulation...
Abstract The interferon signalling system elicits a robust cytokine response against wide range of environmental pathogenic and internal pathological signals, leading to induction subset interferon-induced proteins. We applied DSS (disuccinimidyl suberate) mediated cross-linking mass spectrometry (CLMS) capture novel protein–protein interactions within the realm induced In addition expected proteins, we identified inter- intra-molecular cross-linked adducts for canonical such as MX1, USP18,...
A longstanding disconnect between the growing number of MHC Class I immunopeptidomic studies and genomic medicine hinders cancer vaccine design. We develop COD-dipp to genomically map full spectrum detected canonical non-canonical (non-exonic) antigens from 26 studies. demonstrate that patient mutations in regions overlapping physically identified better predict immunotherapy response when compared neoantigen predictions. suggest a design approach using 140,966 highly immune-visible genome...
Knowledge of the molecular mechanisms underlying interactions between nanomaterials and living systems is fundamental for providing more effective products nanomedicine drug delivery. Controlling response cells/bacteria (such as activation inflammatory processes or apoptosis/necrosis in tumor cells pathogenic bacteria) by tuning specific properties ultimately challenging goal. Notably, this may also provide crucial information assessment any toxic risks induced nanoparticles on humans....
Abstract Cancer treatment was revolutionized by immune checkpoint inhibitors (ICIs) targeting the PD1/PDL1 axis - a brake to system. Despite remarkable success, some patients do not respond therapy or rapidly deteriorate due an unclear mechanism. As reported for several types of tumors, PD1 receptor is solely expressed on cells but also cancer cells. Depending tumor type, it may act either as suppressor promoter. The limited response ICIs in osteosarcoma (OSA), most common type bone cancer,...