A5101503602- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Stress Responses and Cortisol
- Image Retrieval and Classification Techniques
- Advanced Image and Video Retrieval Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Medical Image Segmentation Techniques
- Artificial Intelligence in Healthcare
- Renal Diseases and Glomerulopathies
- Protein Tyrosine Phosphatases
- Diabetes and associated disorders
- Regulation of Appetite and Obesity
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Blood properties and coagulation
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Biosensing Techniques and Applications
- GDF15 and Related Biomarkers
- RNA Research and Splicing
- Apelin-related biomedical research
- HIV/AIDS drug development and treatment
- Big Data and Business Intelligence
- Access Control and Trust
Bharathidasan University
2025
Dasman Diabetes Institute
2013-2022
Diabetes Foundation
2016
King Fahad Specialist Hospital
2013
Xenon Pharmaceuticals (Canada)
2009
National Research Council Canada
2007
Biotechnology Research Institute
2007
McGill University Health Centre
2002-2004
McGill University
2000-2004
Royal Victoria Hospital
2000
GMX1777 is a prodrug of the small molecule GMX1778, currently in phase I clinical trials for treatment cancer. We describe findings indicating that GMX1778 potent and specific inhibitor NAD(+) biosynthesis enzyme nicotinamide phosphoribosyltransferase (NAMPT). Cancer cells have very high rate turnover, which makes modulation an attractive target anticancer therapy. Selective inhibition by NAMPT blocks production results tumor cell death. Furthermore, phosphoribosylated NAMPT, increases its...
Sedentary lifestyle and excessive energy intake are prominent contributors to obesity; a major risk factors for the development of insulin resistance, type 2 diabetes cardiovascular diseases. Elucidating molecular mechanisms underlying these chronic conditions is relevant importance as it might lead identification novel anti-obesity targets. The purpose current study investigate differentially expressed proteins between lean obese subjects through shot-gun quantitative proteomics approach...
ANGPTL8 also called betatrophin is a regulator of lipid metabolism through its interaction with ANGPTL3. It has been suggested to play role in insulin resistance and beta-cell proliferation. Based on function, we hypothesized that will Metabolic Syndrome (MetS). To test this hypothesis designed study measure level subjects MetS as well association high sensitivity C-reactive protein (HsCRP) humans. was measured using ELISA their controls, total 1735 were enrolled. HsCRP examined. higher...
In the passive Heymann nephritis (PHN) model of membranous nephropathy, complement C5b-9 induces glomerular epithelial cell (GEC) injury, proteinuria, and activation cytosolic phospholipase A(2) (cPLA(2)). This study addresses role endoplasmic reticulum (ER) stress proteins (bip, grp94) in GEC injury. that overexpress cPLA(2) (produced by transfection) "neo" (which expresses at a lower level) were incubated with (40 min), leakage constitutively expressed bip grp94 from ER into cytosol was...
Obesity is a major risk factor for myriad of disorders such as insulin resistance and diabetes. The mechanisms underlying these chronic conditions are complex but low grade inflammation alteration the endogenous stress defense system well established. Previous studies indicated that impairment HSP-25 HSP-72 was linked to obesity, diabetes in humans animals while their induction associated with improved clinical outcomes. In an attempt identify additional components heat shock response may be...
Chronic low-grade inflammation and dysregulation of the stress defense system are cardinal features obesity, a major risk factor for development insulin resistance diabetes. Dual-specificity protein phosphatase 1 (DUSP1), known also as MAP kinase (MKP1), is implicated in metabolism energy expenditure. Mice lacking DUSP1 resistant to high-fat diet-induced obesity. However, expression has not been investigated human In current study, we compared pattern between lean obese nondiabetic subjects...
Abstract Spexin is a novel neuropeptide playing an emerging role in metabolic diseases such as obesity and diabetes via involvement energy homeostasis food intake. The present study investigated the effects of type 2 (T2D) on circulating levels spexin its modulation by physical exercise. Normal-weight (n = 50) obese adults with without T2D 69 n 66, respectively) were enrolled study. A subgroup participants 47) underwent supervised 3-month exercise programme. Plasma measured ELISA correlated...
Objectives: To create a robust classification framework to identify and classify the different stages of CKD as per standard medical guidelines based on patient data. develop an accurate predictive model for assessing risk progression from Chronic Kidney Disease (CKD) Failure using Simple Recurrent Neural Network (SRNN). enhance early detection by enabling timely intervention improving outcomes. Methods: obtain right accuracy results, (SRNN) method is used both prediction purposes. The...
In the modern era, big data is a brand-new and developing buzzword. With significant expansion of finance business growth forecast, stock market dynamic, ever-evolving, unpredictable, fascinatingly promising specialty. This study presents novel approach for enhancing forecast accuracy through optimal feature selection combined with deep learning techniques. By employing an Artificial intelligence method to identify select most features influencing prices, we mitigate risks overfitting...
Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes insulin resistance as reflected decrease proteins (HSPs) expression. However, the status HSR non-diabetic human obese not yet elucidated. The aim current study was investigate whether obesity triggers change pattern impact physical exercise on this at protein mRNA levels. Two groups adult subjects...
RANTES and its CCR5 receptor trigger inflammation progression to insulin resistance in obese. In the present study, we investigated for first time effect of physical exercise on expression obese humans. Fifty-seven adult nondiabetic subjects (17 lean 40 obese) were enrolled a 3-month supervised exercise. expressions measured PBMCs subcutaneous adipose tissue before after Circulating plasma levels also investigated. There was significant increase compared lean. PBMCs, however, while mRNA...
The hepatokine fetuin-A is linked to obesity and type 2 diabetes, but its presence expression in adipose tissue remain unclear. In this study, we aimed assess the circulating levels of subcutaneous (SAT) from diabetic non-diabetic obese subjects their modulation by exercise.SAT blood were obtained adults (diabetic, n=118 non-diabetic, n=166) before after a 3-month exercise program n=40 n=36, respectively). Plasma was assayed using ELISA. SAT, peripheral mononuclear cells (PBMCs) cell lines...
Heat shock protein 60 (HSP60) is a key in the crosstalk between cellular stress and inflammation. However, status of HSP60 diabetes obesity unclear. In present study, we investigated adipose tissue obese adults with without changes its expression response to supervised 3-month physical exercise program. Subcutaneous (SAT) blood samples were collected from (n=138 n=92, respectively, at baseline; n=43 for both groups after 3 months exercise). Conventional RT-PCR, immunohistochemistry,...
Abstract Heat shock response (HSR) is an essential host-defense mechanism that dysregulated in obesity-induced insulin resistance and type 2 diabetes (T2D). Our recent data demonstrated DNAJB3 was downregulated obese human subjects showed negative correlation with inflammatory markers. Nevertheless, expression pattern diabetic its mode of action are not yet known. In this study, we reduction transcript protein levels PBMC subcutaneous adipose tissue T2D compared to non-diabetic subjects....
Abstract In the rat passive Heymann nephritis model of membranous nephropathy, complement C5b-9 induces sublethal glomerular epithelial cell (GEC) injury and proteinuria. activates cytosolic phospholipase A2 (cPLA2), products cPLA2-mediated phospholipid hydrolysis modulate GEC present study, we demonstrate that c-Jun N-terminal kinase (JNK) in cultured GECs JNK activity is increased glomeruli isolated from proteinuric rats with nephritis, as compared control rats. Stable overexpression cPLA2...
Glomerular epithelial cell (GEC) injury and apoptosis may contribute to sclerosis in glomerulonephritis. The present study addresses signals that regulate survival of GEC culture the acute puromycin aminonucleoside nephrosis (PAN) model vivo. Compared with on plastic substratum, adhesion collagen increased activation focal kinase (FAK), c-Src, ERK facilitated (prevented apoptosis). exhibited caspase-8 -9 activities, expression proapoptotic protein, Bax, decreased antiapoptotic Bcl-X L ,...
Background . The impact of gender difference on the association between metabolic stress and cardiovascular disease (CVD) remains unclear. We have investigated, for first time, effect oxidative inflammatory responses assessed their correlation with classical cardiometabolites in Arab population. Methods A total 378 adult participants (193 females) were enrolled this cross-sectional study. Plasma levels CRP, IL-6, IL-8, TNF- α , ROS, TBARs, PON1 measured correlated anthropometric...
Epoxide hydrolase 2 (EPHX2) is an emerging therapeutic target in several immunometabolic disorders. EPHX2 metabolizes anti-inflammatory epoxyeicosatrienoic acids into pro-inflammatory diols. The contribution of activity to human obesity remains unexplored. We compared the expression between lean and obese humans (n = 20 each) subcutaneous adipose tissue (SAT) peripheral blood mononuclear cells (PBMCs) using RT-PCR, Western Blot analysis, immunohistochemistry, confocal microscopy before after...
Urocortin3 (UCN3) regulates metabolic functions and is involved in cellular stress response. Although UCN3 expressed human adipose tissue, the association of with obesity diabetes remains unclear. This study investigated effects Type 2 (T2D) increased body weight on circulatory subcutaneous tissue (SAT) levels assessed modulation by a regular physical exercise. Normal-weight (n = 37) overweight adults without T2D 98 n 107, respectively) were enrolled study. A subset subjects 39 for each...
Early stage detection of cancer is the key to provide a better outcome for therapeutic intervention.Most routine screening and diagnosis tools lack sufficient sensitivity and/or specificity sometimes they are invasive.Proteomic technologies hold recently great promise in search new clinical biomarkers early as well discovery targets from accessible bio-specimens.They also have potential contributing understanding biology helping making right decisions patients.Whereas some proteomic...
Abstract The neuropeptide urocortin 3 (UCN3) has a beneficial effect on metabolic disorders, such as obesity, diabetes, and cardiovascular disease. It been reported that UCN3 regulates insulin secretion is dysregulated with increasing severity of obesity diabetes. However, its function in the adipose tissue unclear. We investigated overexpression 3T3-L1 preadipocytes differentiated adipocytes effects heat shock response, ER stress, inflammatory markers, glucose uptake presence...
Phospholipases A2 are enzymes believed to play important roles in numerous physiological systems including sperm cell maturation. Relatively little work has, however, been devoted study these seminal plasma. We therefore undertook the purification and characterization of this enzyme from bovine After a 330-fold purification, an activity corresponding protein 100 kDa was identified by gel filtration. SDS-polyacrylamide electrophoresis analysis purified fraction revealed presence 60-kDa band...