A5022231071- Adipose Tissue and Metabolism
- Exercise and Physiological Responses
- Heat shock proteins research
- Lipid metabolism and disorders
- Stress Responses and Cortisol
- Endoplasmic Reticulum Stress and Disease
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Genetics, Aging, and Longevity in Model Organisms
- GDF15 and Related Biomarkers
- Cancer-related molecular mechanisms research
- Diabetes and associated disorders
- Protein Tyrosine Phosphatases
- Liver Disease Diagnosis and Treatment
- Blood properties and coagulation
- Regulation of Appetite and Obesity
- S100 Proteins and Annexins
- Mitochondrial Function and Pathology
- Spaceflight effects on biology
- Pancreatitis Pathology and Treatment
- Iron Metabolism and Disorders
- Protease and Inhibitor Mechanisms
- Metabolism, Diabetes, and Cancer
- Circadian rhythm and melatonin
- Alcohol Consumption and Health Effects
Dasman Diabetes Institute
2014-2023
University of Helsinki
2022
Sedentary lifestyle and excessive energy intake are prominent contributors to obesity; a major risk factors for the development of insulin resistance, type 2 diabetes cardiovascular diseases. Elucidating molecular mechanisms underlying these chronic conditions is relevant importance as it might lead identification novel anti-obesity targets. The purpose current study investigate differentially expressed proteins between lean obese subjects through shot-gun quantitative proteomics approach...
ANGPTL8 also called betatrophin is a regulator of lipid metabolism through its interaction with ANGPTL3. It has been suggested to play role in insulin resistance and beta-cell proliferation. Based on function, we hypothesized that will Metabolic Syndrome (MetS). To test this hypothesis designed study measure level subjects MetS as well association high sensitivity C-reactive protein (HsCRP) humans. was measured using ELISA their controls, total 1735 were enrolled. HsCRP examined. higher...
Obesity is a major risk factor for myriad of disorders such as insulin resistance and diabetes. The mechanisms underlying these chronic conditions are complex but low grade inflammation alteration the endogenous stress defense system well established. Previous studies indicated that impairment HSP-25 HSP-72 was linked to obesity, diabetes in humans animals while their induction associated with improved clinical outcomes. In an attempt identify additional components heat shock response may be...
Chronic low-grade inflammation and dysregulation of the stress defense system are cardinal features obesity, a major risk factor for development insulin resistance diabetes. Dual-specificity protein phosphatase 1 (DUSP1), known also as MAP kinase (MKP1), is implicated in metabolism energy expenditure. Mice lacking DUSP1 resistant to high-fat diet-induced obesity. However, expression has not been investigated human In current study, we compared pattern between lean obese nondiabetic subjects...
Abstract Spexin is a novel neuropeptide playing an emerging role in metabolic diseases such as obesity and diabetes via involvement energy homeostasis food intake. The present study investigated the effects of type 2 (T2D) on circulating levels spexin its modulation by physical exercise. Normal-weight (n = 50) obese adults with without T2D 69 n 66, respectively) were enrolled study. A subgroup participants 47) underwent supervised 3-month exercise programme. Plasma measured ELISA correlated...
Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes insulin resistance as reflected decrease proteins (HSPs) expression. However, the status HSR non-diabetic human obese not yet elucidated. The aim current study was investigate whether obesity triggers change pattern impact physical exercise on this at protein mRNA levels. Two groups adult subjects...
RANTES and its CCR5 receptor trigger inflammation progression to insulin resistance in obese. In the present study, we investigated for first time effect of physical exercise on expression obese humans. Fifty-seven adult nondiabetic subjects (17 lean 40 obese) were enrolled a 3-month supervised exercise. expressions measured PBMCs subcutaneous adipose tissue before after Circulating plasma levels also investigated. There was significant increase compared lean. PBMCs, however, while mRNA...
The hepatokine fetuin-A is linked to obesity and type 2 diabetes, but its presence expression in adipose tissue remain unclear. In this study, we aimed assess the circulating levels of subcutaneous (SAT) from diabetic non-diabetic obese subjects their modulation by exercise.SAT blood were obtained adults (diabetic, n=118 non-diabetic, n=166) before after a 3-month exercise program n=40 n=36, respectively). Plasma was assayed using ELISA. SAT, peripheral mononuclear cells (PBMCs) cell lines...
Heat shock protein 60 (HSP60) is a key in the crosstalk between cellular stress and inflammation. However, status of HSP60 diabetes obesity unclear. In present study, we investigated adipose tissue obese adults with without changes its expression response to supervised 3-month physical exercise program. Subcutaneous (SAT) blood samples were collected from (n=138 n=92, respectively, at baseline; n=43 for both groups after 3 months exercise). Conventional RT-PCR, immunohistochemistry,...
Abstract Heat shock response (HSR) is an essential host-defense mechanism that dysregulated in obesity-induced insulin resistance and type 2 diabetes (T2D). Our recent data demonstrated DNAJB3 was downregulated obese human subjects showed negative correlation with inflammatory markers. Nevertheless, expression pattern diabetic its mode of action are not yet known. In this study, we reduction transcript protein levels PBMC subcutaneous adipose tissue T2D compared to non-diabetic subjects....
Background . The impact of gender difference on the association between metabolic stress and cardiovascular disease (CVD) remains unclear. We have investigated, for first time, effect oxidative inflammatory responses assessed their correlation with classical cardiometabolites in Arab population. Methods A total 378 adult participants (193 females) were enrolled this cross-sectional study. Plasma levels CRP, IL-6, IL-8, TNF- α , ROS, TBARs, PON1 measured correlated anthropometric...
Epoxide hydrolase 2 (EPHX2) is an emerging therapeutic target in several immunometabolic disorders. EPHX2 metabolizes anti-inflammatory epoxyeicosatrienoic acids into pro-inflammatory diols. The contribution of activity to human obesity remains unexplored. We compared the expression between lean and obese humans (n = 20 each) subcutaneous adipose tissue (SAT) peripheral blood mononuclear cells (PBMCs) using RT-PCR, Western Blot analysis, immunohistochemistry, confocal microscopy before after...
Urocortin3 (UCN3) regulates metabolic functions and is involved in cellular stress response. Although UCN3 expressed human adipose tissue, the association of with obesity diabetes remains unclear. This study investigated effects Type 2 (T2D) increased body weight on circulatory subcutaneous tissue (SAT) levels assessed modulation by a regular physical exercise. Normal-weight (n = 37) overweight adults without T2D 98 n 107, respectively) were enrolled study. A subset subjects 39 for each...
Background: Angiopoietin-like protein 8 (ANGPTL8) is known to regulate lipid metabolism and inflammation. It interacts with ANGPTL3 ANGPTL4 lipoprotein lipase (LPL) activity IKK modulate NF-κB activity. Further, a single nucleotide polymorphism (SNP) leading the ANGPTL8 R59W variant associates reduced low-density lipoprotein/high-density (LDL/HDL) increased fasting blood glucose (FBG) in Hispanic Arab individuals, respectively. In this study, we investigate impact of on inflammatory ANGPTL8....
Abstract The neuropeptide urocortin 3 (UCN3) has a beneficial effect on metabolic disorders, such as obesity, diabetes, and cardiovascular disease. It been reported that UCN3 regulates insulin secretion is dysregulated with increasing severity of obesity diabetes. However, its function in the adipose tissue unclear. We investigated overexpression 3T3-L1 preadipocytes differentiated adipocytes effects heat shock response, ER stress, inflammatory markers, glucose uptake presence...
Background . Cardiovascular disease (CVD) risks persist in patients despite the use of conventional treatments. This might be due to chronic inflammation as reflected epidemiological studies associating circulating low-grade inflammatory markers with CVD recurrent events. Here, we explored this potential link by assessing plasma dual-specificity phosphatase 1 (DUSP1) levels and comparing them high-sensitivity CRP (hsCRP) oxidized low-density lipoprotein (oxLDL) their associations risk...
Purpose Glucagon‐like peptide‐1 (GLP‐1) analogues reduce ER stress and inflammation in key metabolic organs, including the liver. However, their effects on heat shock response (HSR) mitogen‐activated protein kinases (MAPKs) have not yet been elucidated. In present study, we investigate whether GLP‐1 analogue, exendin‐4, triggers expression of HSR increases MAPK activity under stress. Experimental design The exendin‐4 presence or absence palmitic acid (PA; 400 μ m ) glucose (30 HepG2 liver...
The corticotropin-releasing factor neuropeptides (corticotropin-releasing hormone [CRH] and urocortin [UCN]-1,2,3) spexin contribute to the regulation of energy balance inhibit food intake in mammals. However, status these children with overweight has yet be elucidated. This study investigated effect increased body weight on circulating levels neuropeptides.A total 120 a mean age 12 years were enrolled study. Blood samples collected assess correlated various anthropometric, clinical,...
Background Weight-loss surgery is one of the recommended methods for treating obstructive sleep apnea (OSA) in obese patients. While weight reduction critical to relieve symptoms OSA, biochemical factors involved post-surgery improvement are still unknown. We aimed explore link between ANGPTL7 and OSA patients with different severity. Furthermore, we examined effect bariatric on level. Methods quantified levels circulating fasting plasma adipose tissue samples 88 participants before after...
Abstract Background Angiopoietin-like proteins (ANGPTL), primarily 3, 4, and 8, play a major role in maintaining energy homeostasis by regulating triglyceride metabolism. This study evaluated the level of ANGPTL3, 8 liver, brown adipose tissue (BAT), subcutaneous white (SAT) mice maintained under acute chronic cold conditions. Methods C57BL/6J were exposed to temperature (4 °C) for 10 days with food provided ad libitum . Animal tissues harvested at Day 0 (Control group, n = 5) Days 1, 5,...
Cardiovascular disease (CVD) risks persist in patients despite treatment. CVD susceptibility also varies with sex and ethnicity is not entirely explained by conventional risk factors. The aim of the present study was to identify novel candidate markers circulating Peripheral blood mononuclear cells (PBMCs) plasma from Arab obese subjects without using proteomic approaches. Human adults confirmed (n = 208) matched non-CVD controls 152) living Kuwait were examined cross-sectional study....