A5012513454- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Renal and related cancers
- Contact Dermatitis and Allergies
- Climate Change and Health Impacts
- Immunotoxicology and immune responses
- Animal testing and alternatives
- Diet and metabolism studies
- Thermoregulation and physiological responses
- Cancer Immunotherapy and Biomarkers
- Adipose Tissue and Metabolism
- Bariatric Surgery and Outcomes
- Metabolism, Diabetes, and Cancer
- Allergic Rhinitis and Sensitization
- Adipokines, Inflammation, and Metabolic Diseases
- Biochemical Analysis and Sensing Techniques
- Cell Image Analysis Techniques
- Optimism, Hope, and Well-being
- Skin and Cellular Biology Research
- PARP inhibition in cancer therapy
- Acute Lymphoblastic Leukemia research
- Heat shock proteins research
- Advancements in Transdermal Drug Delivery
- vaccines and immunoinformatics approaches
- Atherosclerosis and Cardiovascular Diseases
King Saud bin Abdulaziz University for Health Sciences
2017-2025
National Guard Health Affairs
2022-2024
King Abdullah International Medical Research Center
2017-2024
King Abdulaziz Medical City
2018
Dasman Diabetes Institute
2011-2014
King Faisal Specialist Hospital & Research Centre
2005-2010
University of Amsterdam
1995-2006
Jacobs (United States)
2006
B7-H1 molecule increases the apoptosis of tumorreactive T lymphocytes and reduces their immunogenicity. Breast cancer is second most common cause mortality after lung cancer. Direct evidence linking with has been shown in several malignancies; however, its expression breast not investigated. We used immunohistochemistry to investigate 44 specimens study correlation patients' clinicopathological parameters. The was 22 patients restricted tumor epithelium (15 44, 34% cells), but also expressed...
Abstract Introduction B7-H1 (PD-L1, CD274) is a T cell inhibitory molecule expressed in many types of cancer, leading to immune escape tumor cells. Indeed, previous reports we have shown an association expression with high-risk breast cancer patients. Methods In the current study, used immunohistochemistry, immunofluorescence and Western blot techniques investigate effect neoadjuvant chemotherapy on Results Among tested chemotherapeutic agents, doxorubicin was most effective downregulating...
Heat-related mortality remains health challenges exacerbated by climate change, with sex-based differences in outcomes, yet underlying mechanisms remain poorly understood. This study examined transcriptomic responses to heat exposure peripheral blood mononuclear cells from 19 stroke patients (8 males, mean age 64.8 ± 6.6 years; 11 females, 49.7 years) and controls (11 48.9 9.6 8 44.9 11.8 years). At admission, gene expression revealed upregulation of shock protein genes pathway analysis,...
RANTES and its CCR5 receptor trigger inflammation progression to insulin resistance in obese. In the present study, we investigated for first time effect of physical exercise on expression obese humans. Fifty-seven adult nondiabetic subjects (17 lean 40 obese) were enrolled a 3-month supervised exercise. expressions measured PBMCs subcutaneous adipose tissue before after Circulating plasma levels also investigated. There was significant increase compared lean. PBMCs, however, while mRNA...
Abstract: Skin irritants and contact allergens reduce the number of Langerhans cells (LCs). It has been assumed that this reduction is due their migration to draining lymph node (LN) for initiating immune sensitization in a host. irritation, however, as opposed allergy not considered be an immunological disease. Nevertheless, skin are also known adjuvant‐like effects on allergy, resulting hypersensitivity reactions like toxic dermatitis. The human organotypic explant culture (hOSEC) model...
An evolutionary heat shock response (HSR) protects most living species, including humans, from heat-induced macromolecular damage. However, its role in the pathogenesis of stroke is unknown. We examined whole genome transcriptome peripheral blood mononuclear cells a cohort subjects exposed to same high environmental conditions, who developed (n = 19) versus those did not 19). Patients with had mean rectal temperature at admission 41.7 ± 0.8°C, and eight were deep coma (Glasgow Coma Score 3)....
Abstract Compelling evidences indicate a key role for regulatory T cells (Treg) on the host response to cancer. The Wilms' tumor antigen (WT1) is overexpressed in several human leukemias and thus considered as promising target development of leukemia vaccine. However, recent studies indicated that generation effective WT1-specific cytotoxic can be largely affected by presence Tregs. We have generated T-cell lines clones specifically recognized WT1-84 (RYFKLSHLQMHSRKH) peptide an...
The accelerated migration of Langerhans cells (LCs) out the epidermis and up-regulation maturation markers, upon treatment with subtoxic concentrations chemicals, were used as criteria to determine potential allergenic chemicals capable inducing a hapten-specific delayed-type hypersensitivity reaction. Here we report findings study in which seven coded tested blind fashion, classified contact allergens or non-allergens using human organotypic skin explant culture (hOSEC) model. All that...
The Wilms tumor antigen 1 (WT1) is over-expressed in human leukemias, making it an attractive target for immunotherapy. Most WT1-specific Cytotoxic T Lymphocytes (CTLs) described so far displayed low avidity, limiting its function. To improve the immunogenicity of CTL epitopes, we replaced first-amino-acid two known immunogenic WT1-peptides (126 and 187) with a tyrosine. This modification enhances 126Y analogue-binding ability, triggers significant number IFN-γ-producing cells (P = 0.0003),...
There is an urgent need for the development of leukemia-targeted immunotherapeutic approaches using defined leukemia-associated antigens that are preferentially expressed by most leukemia subtypes and absent or minimally in vital tissues. M-phase phosphoprotein 11 protein (MPP11) extensively overexpressed leukemic cells therefore considered attractive target T cell therapy. We sought to identify potential CD8+ cytotoxic lymphocytes specifically recognised peptides derived from MPP11...
We describe a new alternative method for screening skin irritants by using fresh intact porcine biopsies. Test chemicals were applied to the epidermis of biopsies, which then incubated different times in tissue culture medium at 37°C and with 5% carbon dioxide. A decrease epidermal keratinocyte RNA staining, visualised frozen sections modified methyl-green pyronine (MGP) staining procedure, was employed as marker irritancy. If observed after incubation 4 hours (strong irritant), chemical had...
Animal models are considered to be the “gold standard” for determining potential contact allergenicity of low molecular weight chemicals. However, governmental regulations and ethical considerations limit use animals such purposes. There is therefore a need in vitro alternative models. The human organotypic skin explant culture (HOSEC) model reported promising method predictive testing allergens. accelerated migration Langerhans cells from epidermis upon exposure allergens used identify...
This study investigates the molecular responses to heat stroke in young and old patients by comparing whole-genome transcriptomes between age groups. We analyzed transcriptomic profiles from categorized into two age-defined cohorts: (mean = 44.9 ± 6 years) 66.1 4 years). Control subjects, exposed similar environmental conditions but without developing stroke, were also included analysis provide a baseline for comparison. Despite uniform severity at admission, as indicated core body...
Abstract Context Bariatric surgery has been shown to be effective in inducing complete remission of type 2 diabetes adults with obesity. However, its efficacy achieving remains variable and difficult predict before surgery. Objective We aimed characterize bariatric surgery-induced transcriptome changes associated the predictive role baseline transcriptome. Methods performed a whole-genome microarray peripheral mononuclear cells at (before surgery) 12 months after prospective cohort 26...
Meeting abstracts The Wilms Tumour Antigen 1 (WT1) has been shown to be expressed at low levels in some normal cells. Therefore, many of the potential CTL epitopes against this antigen may absent or suboptimal. To end, different groups introduced modifications sequence anchor
<div>Abstract<p>Compelling evidences indicate a key role for regulatory T cells (T<sub>reg</sub>) on the host response to cancer. The Wilms' tumor antigen (WT1) is overexpressed in several human leukemias and thus considered as promising target development of leukemia vaccine. However, recent studies indicated that generation effective WT1-specific cytotoxic can be largely affected by presence T<sub>regs</sub>. We have generated T-cell lines clones...
Supplement 4 from The Wilms' Tumor Antigen Is a Novel Target for Human CD4<sup>+</sup> Regulatory T Cells: Implications Immunotherapy
Supplement 2 from The Wilms' Tumor Antigen Is a Novel Target for Human CD4<sup>+</sup> Regulatory T Cells: Implications Immunotherapy