A5039580600- Blood transfusion and management
- T-cell and B-cell Immunology
- Ovarian cancer diagnosis and treatment
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Immune Response and Inflammation
- Hemoglobin structure and function
- Renal cell carcinoma treatment
- Blood groups and transfusion
- Monoclonal and Polyclonal Antibodies Research
- Intraperitoneal and Appendiceal Malignancies
- Neonatal Health and Biochemistry
- Blood disorders and treatments
- Respiratory Support and Mechanisms
- Estrogen and related hormone effects
- Radiopharmaceutical Chemistry and Applications
- Hematopoietic Stem Cell Transplantation
- Vascular Malformations Diagnosis and Treatment
- Hepatocellular Carcinoma Treatment and Prognosis
- Endometrial and Cervical Cancer Treatments
- Immunotherapy and Immune Responses
- Medical Imaging Techniques and Applications
- Maternal and fetal healthcare
- Tumors and Oncological Cases
Japanese Red Cross Society, Japan
2007-2022
Tokyo Medical and Dental University
2020
Tokyo Dental College Ichikawa General Hospital
2018-2020
Keio University
2001-2017
Hiroshima University
2012
The University of Tokyo
2009
Kyorin University
2009
Aichi Medical University
2009
DKFZ-ZMBH Alliance
1998
University of South Alabama
1993
summary Recently, evidence implicating the roles of anti‐human leucocyte antigen (HLA) class II antibody in development transfusion‐related acute lung injury (TRALI), which is one most serious possible side effects transfusion, has been accumulating. The aim this study to clarify anti‐HLA DR alloantibody TRALI development. Cultured human microvascular endothelial (LME) cells were incubated with either HLA‐DR15‐positive or HLA‐DR15‐negative monocytes together serum from a single multiparous...
To reduce the risk of transfusion-related acute lung injury (TRALI), plasma products are mainly made from male donors in some countries because lower possibility alloimmunization; other considering this policy. The advantage male-only fresh-frozen (FFP) should be examined a prospective case-control study.This study compared pulmonary function after transfusion FFP derived either only (FFP-male) or mixed (FFP-mixed) informed surgical patients treated at tertiary university hospital Japan....
Antibodies against the human leukocyte antigen (HLA) in donors' blood are implicated development of transfusion-related acute lung injury (TRALI). Screening female donors for HLA antibodies has been introduced to prevent TRALI; however, relationship antibody strength transfused components TRALI not evaluated detail.Donors involved 1038 cases nonhemolytic transfusion reactions (NHTRs) including 283 were screened by fluorescence beads method. specificity and analyzed detail. The usefulness...
Transfusion-related acute lung injury (TRALI) is one of the most serious side-effects transfusion. We report here first two cases TRALI caused by anti-Nak(a) (anti-CD36) antibody from a single blood donor. The aim this study was to clarify role in development.Human microvascular endothelial cells were co-cultured with Nak(a)-positive monocytes and platelets together serum prepared products TRALI-caused antibody-carrying Expressions leukotriene B(4) (LTB(4)) tumour necrosis factor alpha...
SUMMARY Lymphocytes from normal individuals with the histocompatibility antigens HLA-B8 and DR3 have impaired proliferative responses when stimulated suboptimal concentrations of mitogens. We previously shown that an important factor in response is a failure to produce quantities interleukin-2 (IL-2). To examine mechanism decreased responsiveness further, we measured interleukin-1 (IL-1) production low responder subjects compared controls. The peripheral blood mononuelear cells five HLA-B8/...
Background and Objective Antibodies to human leucocyte antigens (HLA) polymorphonuclear neutrophil (PMN) are considered etiologic agents of transfusion‐related acute lung injury (TRALI). The aim this study was clarify the role anti‐HLA DR antibodies in pathophysiology TRALI ability purified soluble HLA (psHLA DR) inhibit release cytokines an vitro model. Materials Methods A coculture pulmonary microvascular endothelial cells (HMVEC) monocytes presence serum containing alloantibodies...
Background Transfusion‐related acute lung injury ( TRALI ) is a life‐threatening complication of blood transfusion. Antibodies against human leukocyte antigens in donors' plasma are the major causes . Several animal models have been developed, and mechanism underlying development has extensively investigated using rodent models. Although sheep nonimmune large‐animal antibody‐mediated not yet available. Study Design Methods To develop swine model , male C lawn strain miniature pigs were used....
SUMMARY Objectives To construct an alternative policy for the donor selection of platelet concentrate ( PC ), a clinical study exploring features lung injury following administration is needed. Background Although male‐donor‐only plasma products appears to have efficiently reduced transfusion‐related acute TRALI this may not be applied because supply shortages. Methods and Materials We prospectively examined pulmonary function after transfusion in informed surgical patients treated at...
Summary A 71‐year‐old Japanese male with myelodysplastic syndrome progressing to overt leukaemia and hepatocellular carcinoma developed dyspnea urticaria immediately after infusion of platelet concentrate (PC). He exhibited an identical reaction following blood transfusion. Serum haptoglobin was undetectable. The patient determined be homozygous for Hp del by polymerase chain (PCR). Antibody detected enzyme‐linked immunosorbent assay (ELISA) Western blot analysis. No antibodies against human...
Background and Objectives Whether transfusion‐associated circulatory overload arises as a simple result of over‐transfusion or requires another trigger remains unclear. Here, we examined whether respiratory distress could be reproduced by massive transfusion alone in an animal model. Materials Methods A total 20 anaesthetized swine were equipped with monitors. Allogeneic blood was obtained from 10 donor swine. 4‐stage loading protocol each stage equivalent to 25% the volume ( BV ) recipient...
Japan is an island country, and the Japanese people have had minimal genetic exchange with other ethnolinguistic groups. Consequently, population highly uniform has limited HLA diversity relative to from countries. However, three groups, distributions differ depending on geographic region. To collect HLA‐rich variety of bone marrow bank donor registrants, it essential know precise distribution in Japan. We analyzed alleles haplotypes based information 177 041 registrants. Registrants were...
Interval debulking surgery (IDS) after neoadjuvant chemotherapy (NAC) is currently one of the preferred treatment options for advanced ovarian, fallopian tube or peritoneal cancer. This study was conducted to evaluate clinical efficacy and safety dose-dense paclitaxel plus carboplatin therapy (ddTC therapy) as NAC these cancers.A retrospective in 25 patients with Stage III/IV cancer who received ddTC NAC. For therapy, (80 mg/m2) administered intravenously on Days 1, 8 15 (AUC 6.0 mg/ml ×...
Abstract Aim To examine the usefulness of neutrophil : lymphocyte (N/L) ratio as a cost‐effective and simple diagnostic marker mature cystic teratoma (MCT) with malignant transformation (MT). Methods A retrospective chart review was performed between 1998 2013 12 MCT patients MT 2009 130 benign MCT. Data were collected on age, tumor size, white blood cell count differential counts, levels, presenting features. Results Older greater higher CA19‐9 or CA125, count, N/L associated univariate...
背景: ハプトグロビン (Hp) 欠損は, 国内における輸血アナフィラキシー発生の一因と考えられている.目的: サンドイッチELISAを用いた簡便なHp欠損の検出法を検討する.方法: 被検患者血清コントロール血清, スタンダードHpを, ウサギ抗ヒトHpポリクローナル抗体を固相化したマイクロプレートに添加して, 10分間反応させ, プレートを洗浄後, 結合したHpをHRP標識抗ヒトHpヤギ血清およびTMB基質で検出した. 被検血清とスタンダードHpの示す吸光度を比較して患者がHp欠損か否かを判定した.結果: 上記の方法で, 血清中に存在する3μg/dL以上の濃度のHpを検出できた. また, ピークレートネフェロメトリー法で血清Hp含量が5mg/dL以下と測定された患者の中から, 先天性のHp欠損者を検出することができた. 判定は肉眼でも可能であった.結論: 本法は, 操作が簡便で, 検出感度が高い. 本法を用いれば先天性Hp欠損者と二次的に血中Hp含量が減少した患者の鑑別が可能である. 頻回輸血を必要とする患者の輸血時のアナフィラキシー発生の予防や,...
To address the role of cancer‐stroma interactions, we performed gene expression profiling both cancer and stroma, using matching samples endometrial (EC), analyzed relationship between pattern prognosis in EC. Sixty EC cases were included this study (38 nonrecurrent 22 recurrent). Cancer stroma separated by performing laser capture microdissection, microarray analysis was separately on stromal cells. Genes related with progression‐free survival (PFS) Cox regression model, established a...
Abstract Background and Objectives In Japan, cord blood is used for more than half of all unrelated stem cell transplantations. The public banks (CBBs) have been collecting information on transplantation‐related adverse events from physicians a voluntary basis, without common definitions the reactions. aims this study were to compare two classification systems improve reporting system clarify actual risk infusion, which can then provide impetus take appropriate measures reduce events....
Abstract Objective this prospective cohort study aimed to assess the safety and efficacy of bevacizumab combined with chemotherapy in Japanese patients relapsed ovarian, fallopian tube or primary peritoneal cancer. Methods study, 40 cancer selected receive were enrolled. Patients poor general condition excluded. Each patient was monitored prospectively for adverse events, administration status, disease status survival. Treatment continued until intolerable events progression. The endpoint...
Antibodies to β 2 ‐microglobulin (β m), the light‐chain component of class I histocompatibility antigens, provide a strong co‐stimulatory signal human lymphocytes in presence phorbol ester. This activation requires high concentration it antibody, whereas effect on responses mitogenic lectin is exclusively inhibitory over broad dose range. A rabbit polyclonal and mouse monoclonal antibody m were similarly co‐stimulatory. Antibody was with ester, but not calcium ionophore, suggesting that...