A5102815610- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Peroxisome Proliferator-Activated Receptors
- Hepatitis C virus research
- Alcohol Consumption and Health Effects
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- Computational Drug Discovery Methods
- Multiple Myeloma Research and Treatments
- Biotin and Related Studies
- Protein Degradation and Inhibitors
- Cancer-related molecular mechanisms research
- Blood Coagulation and Thrombosis Mechanisms
- Liver Diseases and Immunity
- Diet, Metabolism, and Disease
- Renin-Angiotensin System Studies
- Cytokine Signaling Pathways and Interactions
- Pulmonary Hypertension Research and Treatments
- Food composition and properties
- Hepatocellular Carcinoma Treatment and Prognosis
- Liver Disease and Transplantation
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Lipid metabolism and disorders
- Click Chemistry and Applications
Hebei Medical University
2015-2025
Third Hospital of Hebei Medical University
2014-2025
Nanjing Agricultural University
2021-2024
Affiliated Hospital of Youjiang Medical University for Nationalities
2023
Huzhou Academy of Agricultural Sciences
2023
Chinese Medical Association
2021-2022
Sichuan University
2022
AbbVie (United States)
2016-2018
Abbott Fund
2002-2013
Abbott (United States)
2007-2011
Angiotensin-converting enzyme is involved in apoptosis of alveolar epithelial cells (Wang R, Zagariya A, Ang E, Ibarra-Sunga O, and Uhal BD. Am J Physiol Lung Cell Mol 277: L1245–L1250, 1999). This study tested the ability angiotensin-converting inhibitor captopril or caspase Z-Val-Ala-Asp-fluoromethylketone (ZVAD-fmk) to block cell lung fibrosis vivo response bleomycin (Bleo). Male Wistar rats received 8 U/kg Bleo (bleomycin sulfate) vehicle intratracheally. Subgroups Bleo-treated...
Recent work from this laboratory demonstrated potent inhibition of apoptosis in human alveolar epithelial cells (AECs) by the angiotensin-converting enzyme inhibitor captopril [B. D. Uhal, C. Gidea, R. Bargout, A. Bifero, O. Ibarra-Sunga, M. Papp, K. Flynn, and G. Filippatos. Am. J. Physiol. 275 ( Lung Cell. Mol. 19): L1013–L1017, 1998]. On basis, we hypothesized that cell type might be induced angiotensin II (ANG II) through its interaction with ANG receptor. Purified dose-dependent both...
The development of bromodomain and extraterminal domain (BET) inhibitors their examination in clinical studies, particularly oncology settings, has garnered substantial recent interest. An effort to generate novel BET with excellent potency drug metabolism pharmacokinetics (DMPK) properties was initiated based upon elaboration a simple pyridone core. Efforts develop bidentate interaction critical asparagine residue resulted the incorporation pyrrolopyridone core, which improved by 9–19-fold....
Objective. Hepatic oxidative stress plays a key role in the development of non-alcoholic steatohepatitis (NASH). However, protective effects antioxidants on NASH are largely unknown. The aim this study was to elucidate effect and mechanism mice. Material methods. C57BL6/J mice were fed methionine-choline-deficient (MCD) diet for 10 days or 3 weeks induce steatohepatitis. Antioxidants (vitamin E, ABT, vitamin E plus ABT) supplemented MCD diet, respectively. apoptosis assessed, activation...
Abstract Nonalcoholic fibrosing steatohepatitis is a uniform process throughout nonalcoholic fatty liver disease (NAFLD). MicroRNAs (miRNAs) have been suggested to modulate cellular processes in diseases. However, the functional role of miRNAs largely unclear. In this study, we systematically analyzed hepatic by microarray analysis C57BL/6J mice induced methionine-choline deficient (MCD) diet. We identified 19 up-regulated and 18 down-regulated with fibrosis. Among these dysregulated miRNAs,...
Nonalcoholic fibrosing steatohepatitis is a uniform process that occurs throughout nonalcoholic fatty liver disease (NAFLD). MicroRNAs (miRNAs) have been shown to be involved in the biological processes, but role and molecular mechanism of miRNAs NAFLD are not entirely clear. In this study, we observed significant reduction expression miR-130a-3p livers mouse model with fibrosis induced by methionine-choline-deficient diet, patients, activated hepatic stellate cells (HSCs). A dual-luciferase...
Members of the BET family bromodomain containing proteins have been identified as potential targets for blocking proliferation in a variety cancer cell lines. A two-dimensional NMR fragment screen binders to bromodomains BRD4 phenylpyridazinone with weak binding affinity (1, Ki = 160 μM). SAR investigation 1, aided by X-ray structure-based design, enabled synthesis potent pyridone and macrocyclic inhibitors exhibiting single digit nanomolar potency both biochemical based assays. Advanced...
Recent works from this laboratory demonstrated potent inhibition of Fas-induced apoptosis in alveolar epithelial cells (AECs) by the angiotensin-converting enzyme (ACE) inhibitor captopril [B. D. Uhal, C. Gidea, R. Bargout, A. Bifero, O. Ibarra-Sunga, M. Papp, K. Flynn, and G. Filippatos. Am. J. Physiol. 275 ( Lung Cell. Mol. 19): L1013–L1017, 1998] induction dose-dependent AECs purified angiotensin (ANG) II [R. Wang, Zagariya, E. Ang, S. Deshmukh, Chaudhary, Baraboutis, Filippatos B. Uhal....
Recent work from this laboratory demonstrated that apoptosis of pulmonary alveolar epithelial cells (AEC) in response to Fas requires angiotensin II (ANGII) generation de novo and binding its receptor (Wang et al., 1999b, Am J Physiol Lung Cell Mol 277:L1245-L1250). These findings led us hypothesize a similar mechanism might be involved the induction AEC by TNF-alpha. Apoptosis was detected assessment nuclear chromatin morphology, increased activity caspase 3, annexin V, net cell loss...
Previous work from this laboratory demonstrated induction of apoptosis in lung alveolar epithelial cells (AEC) by purified angiotensin II (ANG II) and expression mRNAs for both ANG receptor subtypes AT 1 2 (Wang R, Zagariya A, Ibarra-Sunga O, Gidea C, Ang E, Deshmukh S, Chaudhary G, Baraboutis J, Filippatos Uhal BD. Am J Physiol Lung Cell Mol 276: L885–L889, 1999.). The present study was designed to determine the subtype mediating AEC response II. Apoptosis induced with applied human...
Yue-Min Nana*, Na Fua, Wen-Juan Wua, Bao-Li Lianga, Rong-Qi Wanga, Su-Xian Zhaoa, Jing-Min Zhaob & Jun Yucda Department of Traditional and Western Medical Hepatology, the Third Hospital Hebei University, Shijiazhuang, Chinab Pathology, Beijing 302 Hospital, Beijing, Chinac Institute Digestive Disease Li Ka Shing Health Sciences, Medicine Therapeutics, The Chinese University Hong Kong, Kongd Gastroenterology, First China
Abstract Background Peroxisome proliferator activated receptor alpha (PPARα) regulates lipids metabolism and inhibits inflammatory response. However, the role of PPARα in alcoholic liver disease is largely unknown. We aim to elucidate effect molecular basis ethanol induced hepatic injury mice. Results C57BL/6J mice fed with 4% ethanol-containing Lieber-DeCarli liquid diet for 12 weeks exhibited hepatocyte steatosis, necrosis infiltration, accompanied elevated serum alanine aminotransferase...
Peroxisome proliferator activated receptor alpha (PPARα) ameliorates ethanol induced hepatic steatohepatitis. However, its role in alcoholic liver fibrosis has not been fully clarified. The aim of this study was to elucidate the effect and molecular basis PPARα mice. C57BL/6J mice were fed with 4% ethanol-containing Lieber-DeCarli liquid diet for eight weeks, intraperitoneal injected 5% carbon tetrachloride (CCl4) last four weeks induce fibrosis. agonist WY14643 administered during couple...
Abstract: Earlier work in this laboratory showed that amiodarone induces apoptosis alveolar epithelial cells by a mechanism inhibitable angiotensin system antagonists. A variety of recent studies suggests critical role for cell the pathogenesis lung fibrosis. On basis we hypothesized amiodarone‐induced and fibrosis vivo might be converting enzyme inhibitor captopril or receptor antagonist losartan. Amiodarone‐induced was induced male Wistar rats oral adminstration over six months. Replicate...
The antiarrhythmic amiodarone (AM) and its metabolite desethylamiodarone (Des) are known to cause AM-induced pulmonary toxicity, but the mechanisms underlying this disorder remain unclear. We hypothesized that AM might toxicity in part through induction of apoptosis or necrosis alveolar epithelial cells (AECs). Two models type II pneumocytes, human AEC-derived A549 cell line primary AECs isolated from adult Wistar rats, were incubated with Des for 20 h. Apoptotic determined by morphological...
Objective. The pathogenesis of non-alcoholic steatohepatitis is still unclear. We have demonstrated previously that peroxisome proliferator activated receptor gamma (PPARγ) ligand protects against inflammation and fibrogenesis in experimental steatohepatitis. aim to elucidate the effect mechanism PPARγ itself on nutritional fibrotic mice. Methods. C57BL/6J mice were fed with methionine-choline deficient (MCD) diet for 8 weeks induce Mice MCD treated adenovirus carrying (Ad-PPARγ), Ad-PPARγ...
A structurally novel acetyl-CoA carboxylase (ACC) inhibitor is identified from high-throughput screening. preliminary structure-activity relationship study led to the discovery of potent dual ACC1/ACC2 and ACC2 selective inhibitors against human recombinant ACC1 ACC2. Selective exhibited IC50<20 nM >1000-fold selectivity ACC1. (S)-Enantiomer 9p high activity lowered muscle malonyl-CoA dose-dependently in acute rodent studies, whereas (R)-enantiomer 9o was weak had no effect on level.
Successful siRNA therapeutics requires the optimal integration of multiple components, including an efficient delivery system, a disease indication that is appropriate for siRNA-based therapy, and potent nontoxic against robust therapeutic target. Although all currently available systems have limitations, it important to recognize careful selection indication, target, molecule could partially compensate deficiencies associated with system makes possible advance regimen. In this study, we...
Interleukin-23 (IL-23) and its downstream factor IL-17 are the key cytokines involved in immune inflammatory response chronic liver diseases. This study aimed to investigate role molecular mechanisms of IL-23/Th17 axis hepatitis C virus (HCV) infection, efficacy modulation anti-HCV therapy. Sixty-six HCV-infected patients 20 healthy controls were enrolled. The received PegIFNa-2a ribavirin therapy for at least 48 weeks. plasma level IL-23 number IL-17A-, IFN-γ-, IL-21-producing peripheral...