A5078375338- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Phylogenetic Studies
- Pesticide Residue Analysis and Safety
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Analytical chemistry methods development
- Adsorption and biosorption for pollutant removal
- Extraction and Separation Processes
- Rangeland Management and Livestock Ecology
- Plant Ecology and Soil Science
- Chemical Synthesis and Characterization
- RNA and protein synthesis mechanisms
- Analytical Chemistry and Chromatography
- Genetic Mapping and Diversity in Plants and Animals
- Saffron Plant Research Studies
- Environmental Quality and Pollution
- Botany and Plant Ecology Studies
- Pharmacological Effects and Assays
- Phosphorus and nutrient management
- Bacillus and Francisella bacterial research
- Plant Ecology and Taxonomy Studies
- Photosynthetic Processes and Mechanisms
- Pesticide and Herbicide Environmental Studies
- Environmental Changes in China
Jilin Agricultural University
2022-2024
Harbin Normal University
2023-2024
SLAC National Accelerator Laboratory
2023-2024
Lanzhou University
2024
Stanford University
2023-2024
Sichuan Academy of Agricultural Sciences
2009-2023
Pennsylvania State University
2017-2022
Lanzhou Institute of Husbandry and Pharmaceutical Sciences
2021-2022
Chinese Academy of Agricultural Sciences
2021-2022
Yale University
2017-2021
Abstract The model organism Encylopedia of Regulatory Elements (modERN) project was designed to generate genome-wide binding profiles for the majority transcription... To develop a catalog regulatory sites in two major organisms, Drosophila melanogaster and Caenorhabditis elegans, modERN (model Encyclopedia Networks) consortium has systematically assayed transcription factors (TFs). Combined with data produced by our predecessor, modENCODE (Model Organism ENCyclopedia Of DNA Elements), we...
A novel, “all-in-one”, multifunctional microsphere with a fluorescent mesoporous silica shell (Rhodamine B coordinate receptor inside) and magnetic core (Fe3O4) has been successfully fabricated using sol–gel method small molecular (CTAB) surfactants as structure-directing agents. At the same time, they were examined for environmental protection applications to detect, adsorb remove Hg2+ in aqueous solution. The prepared nanocomposite microspheres fluorescent, mesoporous, magnetizable,...
Bacillus thuringiensis is a well-known entomopathogenic bacterium used worldwide as an environmentally compatible biopesticide. During sporulation, B. accumulates large number of parasporal crystals consisting insecticidal crystal proteins (ICPs) that can account for nearly 20-30% the cell's dry weight. However, metabolic regulation mechanisms ICP synthesis remain to be elucidated. In this study, combined efforts in transcriptomics and proteomics mainly uncovered following 6 mechanisms: (1)...
Abstract Chiglitazar is a novel peroxisome proliferator-activated receptor (PPAR) pan-agonist, which passed phase III clinical trials and was newly approved in China for use as an adjunct to diet exercise glycemic control adult patients with Type 2 Diabetes (T2D). To explore the circulating protein signatures associated administration of chiglitazar T2D patients, we conducted comparative longitudinal study using plasma proteome profiling. Of 157 included study, administered specific group,...
The nonpathogenic bacterium Mycobacterium smegmatis mc2155 has been widely used as a model organism in mycobacterial research, yet detailed study about its transcription landscape remains to be established. Here we report the transcriptome, expression profiles and transcriptional structures through growth-phase-dependent RNA sequencing (RNA-seq) well other related experiments. We found: 1) 2139 start sites (TSSs) genome-wide scale, of which eight samples were randomly selected further...
The wide variety of specialized permissive and repressive mechanisms by which germ cells regulate developmental gene expression are not well understood genome-wide. Isolation with high integrity purity from living animals is necessary to address these open questions, but no straightforward methods currently available. Here we present an experimental paradigm that permits the isolation nuclei C. elegans at quantities sufficient for genomic analyses. We demonstrate represent a very pure...
Abstract Sequence verification of plasmid DNA is critical for many cloning and molecular biology workflows. To leverage high-throughput sequencing, several methods have been developed that add a unique barcode to individual samples prior pooling sequencing. However, these require an extraction and/or in vitro barcoding reaction each sample processed, limiting throughput adding cost. Here, we develop arrayed vivo platform enables pooled library preparation Oxford Nanopore This method has high...
In bacteria, both promoters and 5'-untranslated regions (5'-UTRs) of mRNAs play vital regulatory roles in gene expression. this study, we identified 1203 active promoter candidates Bacillus thuringiensis through analysis the genome-wide TSSs based on transcriptome data. There were 11 types σ-factor 34 transcription factor binding sites found 723 1097 candidates, respectively. Moreover, within transcriptional units (TUs), most (52%) 5'-UTRs 10-50 nucleotides length, 12.8% TUs had a long...
Bacillus thuringiensis, a Gram-positive endospore-forming bacterium, is characterized by the formation of parasporal crystals consisting insecticidal crystal proteins (ICPs) during sporulation. We reveal gene expression profiles and regulatory mechanisms associated with spore based on transcriptomics proteomics data B. thuringiensis strain CT-43. During sporulation, five ICP genes encoded CT-43 were specifically transcribed; moreover, most structure-, assembly-, maturation-associated...
Carbon nanotubes fabricated by the dc arc discharge method (ADCNTs) and chemical vapor deposition (CVDCNTs) were oxidized with concentrated HNO 3 to modify their surface chemistry. The materials characterized using SEM, TEM, FTIR, XPS, potentiometric titration, nitrogen adsorption. initial used as adsorbents of cadmium from aqueous solutions different pH. Langmuir Freundlich adsorption models applied fit isotherm data, both experimental data very well. acid oxidation resulted in an increase...
Abstract To investigate single nucleotide polymorphism (SNP) loci associated with yearling wool traits of fine-wool sheep for optimizing marker-assisted selection and dissection the genetic architecture traits, we conducted a genome-wide association study (GWAS) based on fixed random model circulating probability unification (FarmCPU) staple length (YSL), mean fiber diameter (YFD), greasy fleece weight (YGFW), clean rate (YCFR) by using whole-genome re-sequenced data (totaling 577 sheep)...