A5012487605- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- Viral gastroenteritis research and epidemiology
- CRISPR and Genetic Engineering
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Parvovirus B19 Infection Studies
- Adolescent Sexual and Reproductive Health
- HIV/AIDS Research and Interventions
- Liver physiology and pathology
- Mosquito-borne diseases and control
- Developmental Biology and Gene Regulation
- Sphingolipid Metabolism and Signaling
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Mesenchymal stem cell research
- HIV Research and Treatment
- Hedgehog Signaling Pathway Studies
- Tuberculosis Research and Epidemiology
- Intestinal and Peritoneal Adhesions
- Intestinal Malrotation and Obstruction Disorders
- Sex work and related issues
National Institute for Viral Disease Control and Prevention
2016-2025
Chinese Center For Disease Control and Prevention
2000-2025
National Health and Family Planning Commission
2025
Chinese Academy of Sciences
2019-2022
Wuhan Institute of Virology
2019-2022
Center for Disease Control
2000-2022
State Key Laboratory of Infectious Disease Prevention and Control
2017
University of Washington Medical Center
2013-2017
Guangxi Center for Disease Prevention and Control
2017
Qinghai Institute for Endemic Diease Prevention and Control
2016
An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV) should confer sustained, protective immunity at both systemic and levels. Here, we evaluated the in vivo antigen-specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) 41 (Ad41) -based vaccines expressing MERS-CoV spike (S) protein. Intragastric either Ad5-S Ad41-S IgG neutralizing antibody serum; however, T-cell...
The development of an effective vaccine is critical for prevention a Middle East respiratory syndrome coronavirus (MERS-CoV) pandemic. Some studies have indicated the receptor-binding domain (RBD) protein MERS-CoV spike (S) good candidate antigen subunit vaccine. However, highly purified proteins are typically not inherently immunogenic. We hypothesised that humoral and cell-mediated immunity would be improved with modification vaccination regimen. Therefore, immunogenicity novel RBD-based...
The development of broad-spectrum universal influenza vaccines and optimisation vaccination strategies to address the threats posed by pandemics emerging viruses are critical for public health. In this study, an adenovirus type 5 vector-based vaccine carrying hemagglutinin (HA) stem H1, HA H3, neuraminidase N1 from virus was constructed. Immune responses were evaluated in mice using various strategies: prime-only (intramuscular [IM] or intranasal [IN]) prime-boost (IM+IN). Compared strategy,...
ABSTRACT The development of broad‐spectrum universal influenza vaccines and optimization vaccination strategies to address the threats posed by pandemics emerging viruses are critical for public health. In this study, an adenovirus type 5 vector‐based vaccine carrying hemagglutinin (HA) stem H1, HA H3, neuraminidase (NA) N1 from virus was constructed. Immune responses were evaluated in mice using various strategies: prime‐only (intramuscular [IM] or intranasal [IN]) prime‐boost (IM + IN)....
A central resistance mechanism in solid tumors is the maintenance of epithelial junctions between malignant cells that prevent drug penetration into tumor. Human adenoviruses (Ads) have evolved mechanisms to breach barriers. For example, during Ad serotype 3 (Ad3) infection tumor cells, massive amounts subviral penton-dodecahedral particles (PtDd) are produced and released from infected trigger transient opening junctions, thus facilitating lateral virus spread. We show here an Ad3 mutant...
Human adenovirus serotypes Ad3, Ad7, Ad11, and Ad14 use the epithelial junction protein desmoglein 2 (DSG2) as a receptor for infection. During Ad infection, fiber penton base capsid proteins are produced in vast excess form hetero-oligomers, called pentons. It has been shown Ad3 that pentons self-assemble into penton-dodecahedra (PtDd). Our previous studies with recombinant purified PtDd (produced insect cells) showed bind to DSG2 trigger intracellular signaling resulting transient opening...
Gene therapy and vaccine development need more novel adenovirus vectors. Here, we attempt to provide strategies construct vectors based on restriction-assembly for researchers with little experience in this field. Restriction-assembly is a combined method of restriction digestion Gibson assembly, by which the major part obtained plasmid comes from digested DNA fragments instead PCR products. We demonstrated capability manipulating genome simian 1 (SAdV-1) study. A product backbone was SAdV-1...
Severe fever with thrombocytopenia syndrome virus (SFTSV) is a newly identified phlebovirus associated severe hemorrhagic in humans. Studies have shown that SFTSV nucleoprotein (N) induces BECN1-dependent autophagy to promote viral assembly and release. However, the function of other proteins regulating has not been reported. In this study, we identify NSs, nonstructural protein forms viroplasm-like structures cytoplasm infected cells as component mediating SFTSV-induced autophagy. We found...
To evaluate the patterns, related factors and prognostic value of abnormal magnetic resonance angiography (MRA) in human immunodeficiency virus negative tuberculous meningitis.We performed a prospective study patients aged >14 years. Abnormality on MRA was correlated with clinical, laboratory imaging findings. Modified Barthel index used to assess outcome at 6 months after inclusion.Of 101 included, 45 (44.6%). The distribution abnormality classified as disseminated irregular calibres...
Summary Interleukin 6 (IL‐6) influences the growth and survival of multiple myeloma (MM) cells via activation signalling cascades. Although sphingosine kinase (SPHK) is known to play important roles in regulation cell proliferation apoptosis, role SPHK IL‐6 pathology MM remains unclear. This study found that activated cells, which mediates suppressive effects on apoptosis. Both lines primary constitutively expressed SPHK, treatment with resulted a concentration‐dependent manner. Specific...
Fibre is the viral protein that mediates attachment and infection of adenovirus to host cell. Fowl 4 (FAdV-4) possesses two different fibre trimers on each penton capsomere, roles separate fibres remain elusive. Here, we attempted investigate function FAdV-4 by using reverse genetics approaches. Adenoviral plasmids carrying fiber1 or fiber2 mutant genes were constructed used transfect chicken LMH cells. Fiber1-mutated recombinant virus could not be rescued. Such defective phenotype was...
Defensins are small antimicrobial peptides capable of neutralizing human adenovirus (HAdV) in vitro by binding capsid proteins and blocking endosomal escape virus. In humans, the alpha defensin HD5 is produced specialized epithelial cells gastrointestinal genito-urinary tracts. Here, we demonstrate, using patient biopsy specimens, that also expressed as an active, secreted peptide ovarian lung cancer situ This finding prompted us to study role infection spread replication-competent,...
Abstract Background Human adenovirus serotype 41 (Ad41) is a natural pathogen of the digestive tract and can cause gastroenteritis. There has been interest in reconstructing Ad41 as gene delivery vector targeting gastrointestinal tract, which hampered by its fastidiousness. Methods An E1B55K‐transduced 293 cell line (293E12) was established packaging line. A backbone plasmid (pAdbone41) shuttle (pSh41‐CMV) were constructed based on genome. Replication‐defective (Ad41‐GFP) rescued 293E12...
Current passive case finding strategies are not effective at identifying tuberculosis (TB) patients in rural China.To evaluate a community-based, active (ACF) scheme symptomatic individuals with TB.We conducted door-to-door household visits of all residents aged 15 years two sites to screen for TB symptoms. Individuals symptoms were enrolled and asked provide three sputum samples. All participants underwent chest X-ray, microbiologic detection Mycobacterium from samples using microscopy,...
A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of whole right and partial left ends FAdV-4 genome, which worked through cell-free reactions restriction digestion Gibson assembly. Three recombinant viruses were constructed test assumption that species-specific viral genes ORF4 ORF19A might be responsible enhanced virulence: ORF1, ORF1b ORF2 replaced...
Mouse adenoviruses (MAdV) play important roles in studying host–adenovirus interaction. However, easy-to-use reverse genetics systems are still lacking for MAdV. An infectious plasmid pKRMAV1 was constructed by ligating genomic DNA of wild-type MAdV-1 with a PCR product containing backbone through Gibson assembly. A fragment excised from restriction digestion and used to generate intermediate pKMAV1-ER, which contained E3, fiber, E4, E1 regions MAdV-1. CMV promoter-controlled GFP expression...