A5011081349- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Peroxisome Proliferator-Activated Receptors
- Animal Nutrition and Physiology
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Aquatic Invertebrate Ecology and Behavior
- Molecular Biology Techniques and Applications
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Underwater Acoustics Research
- Maritime and Coastal Archaeology
- Marine Biology and Ecology Research
- Metabolomics and Mass Spectrometry Studies
- Aquaculture disease management and microbiota
- Blood Coagulation and Thrombosis Mechanisms
- Cytokine Signaling Pathways and Interactions
- Meat and Animal Product Quality
- Marine Bivalve and Aquaculture Studies
- Chronic Disease Management Strategies
- Invertebrate Immune Response Mechanisms
- Monoclonal and Polyclonal Antibodies Research
Chinese PLA General Hospital
2025
Henan Agricultural University
2019-2024
Ministry of Natural Resources
2022-2023
Qingdao Institute of Marine Geology
2023
Qingdao National Laboratory for Marine Science and Technology
2022-2023
Center for Disease Control
2023
China Geological Survey
2022
Hainan University
2021
Our previous studies showed that microRNA-15a (miR-15a) was closely related to intramuscular fat (IMF) deposition in chickens; however, its regulatory mechanism remains unclear. Here, we evaluated the expression characteristics of miR-15a and relationship with acetyl-CoA acyltransferase 1 (ACAA1), acyl-CoA oxidase (ACOX1) sterol carrier protein 2 (SCP2) by qPCR analysis Gushi chicken breast muscle at 6, 14, 22, 30 weeks old, where performed transfection tests mimics preadipocytes verified...
Fatty acids composition in poultry muscle is directly related to its tenderness, flavour, and juiciness, whereas genetic mechanisms have not been elucidated. In this study, the structure key regulatory genes of breast fatty acid local Chinese chicken, Gushi-Anka F2 resource population by integrating genome-wide association study (GWAS) weighted gene co-expression network analysis (WGCNA) strategies. GWAS was performed based on 323,306 single nucleotide polymorphisms (SNPs) obtained...
The development of abdominal fat and meat quality are closely related can impact economic efficiency. In this study, we conducted transcriptome sequencing the tissue Gushi chickens at 6, 14, 22, 30 weeks, selected key miRNA-mRNA regulatory networks to through correlation analysis.A total 1893 differentially expressed genes were identified. Time series analysis indicated that around 6 chicken was extensively regulated by TGF-β signaling pathway, Wnt PPAR pathway. However, weeks age, apoptosis...
Abstract Background Serum biochemical indicators are often regarded as direct reflections of animal metabolism and health. The molecular mechanisms underlying serum chicken (Gallus Gallus) have not been elucidated. Herein, we performed a genome-wide association study (GWAS) to identify the variation associated with indicators. aim this research was broaden understanding in chickens. Results A GWAS carried out on 734 samples from an F2 Gushi× Anka population. All chickens were genotyped by...
The characteristics of muscle fibers determine the growth and meat quality poultry. In this study, we performed a weighted gene co-expression network analysis (WGCNA) on fiber transcriptome profile breast tissue Gushi chicken at 6, 14, 22, 30 weeks.
Circular RNAs (circRNAs) play important roles in adipogenesis. However, studies on circRNA expression profiles associated with the development of abdominal adipose tissue are lacking chickens. In this study, 12 cDNA libraries were constructed from Chinese domestic Gushi chickens at 6, 14, 22, and 30 weeks. A total 1,766 circRNAs identified by Illumina HiSeq 2500 sequencing. These primarily distributed chr1 through chr10 sex chromosomes, 84.95% gene exons. Bioinformatic analysis showed that...
Abstract Background Chicken skeletal muscle is an important economic product. The late stages of chicken development constitute the main period that affects meat production. LncRNAs play roles in controlling epigenetic process growth and development. However, studies on role lncRNAs breast are still lacking. In this study, to investigate expression characteristics during development, 12 cDNA libraries were constructed from Gushi samples 6-, 14-, 22-, 30-week-old chickens. Results A total...
Several recent studies investigated the role of miR-29 family in muscle development. However, only a few focused on chicken skeletal muscle. In present study, cell cycle, 5-ethynyl-2'-deoxyuridine (EdU), counting kit-8 (CCK-8), and other assays indicated that miR-29b-1-5p can inhibit proliferation primary myoblasts (CPMs); western blot assay immunofluorescence detection MYHC demonstrated promote differentiation myoblasts. The functional enrichment analysis revealed target genes may be...
Circular RNAs (circRNAs) play a significant regulatory role during skeletal muscle development. To identify circRNAs postnatal development in chickens, we constructed 12 cDNA libraries from breast tissues of Chinese Gushi chickens at 6, 14, 22, and 30 weeks performed RNA sequencing. In total, 2112 were identified, among them 79.92% derived exons. CircRNAs are distributed on all chromosomes especially 1–9 Z. Bioinformatics analysis showed that each circRNA had an average 38 miRNA binding...
1. Chicken muscle is an important factor in meat quality and its development controlled by a complex regulatory network.2. The following study examined the expression of miR-30a-3p Gushi chicken breast tissue found that it was differentially expressed at different embryonic stages, reaching peak 14-day-old embryo (E14).3. effect on primary myoblasts (CPMs) explored. Results from both cell counting kit-8 (CCK-8) 5-ethynyl-2'-deoxyuridine (EdU) showed this can inhibit proliferation myoblasts,...
Abstract Background The composition and content of fatty acids in the breast muscle are important factors influencing meat quality. In this study, we investigated acid Gushi chickens at different developmental stages (14 weeks, 22 30 weeks). Additionally, utilized transcriptomic data from same tissue employed WGCNA module identification methods to identify key genes associated with chicken elucidate their regulatory networks. Results Among them, six modules (blue, brown, green, light yellow,...
The development and egg-laying performance of hens are precisely regulated by hormones secreted the pituitary. In this study, we performed comprehensive transcriptome sequencing pituitary from Hy-Line Brown at 15, 20, 30 68 W age. Through association analysis, identified key genes ceRNA regulatory networks related to egg production. Based on data, 470 differentially expressed lncRNAs (DE-lncRNAs), 38 miRNAs (DE-miRNAs), 2,449 mRNAs (DE-mRNAs). Time-series analysis pinpointed signaling...
Chickens are one of the most important sources meat worldwide, and growth status abdominal fat is closely related to production efficiency. Long noncoding RNAs (lncRNAs) play an role in lipid metabolism deposition regulation. However, research on expression profile lncRNAs development chickens after hatching their interaction regulatory networks still lacking. To characterize lncRNA during chicken fat, adipose tissues from 6-, 14-, 22-, 30-week-old Chinese Gushi were herein used construct 12...
Deep-sea mussels living in the cold seeps with enormous biomass act as primary consumers. They are well adapted to extreme environment where light is absent, and hydrogen sulfide, methane, other hydrocarbon-rich fluid seepage occur. Despite previous studies on diversity, role, evolution, symbiosis, changing adaptation patterns during different developmental stages of deep-sea remain largely unknown.The (Bathymodiolus platifrons) two were collected from seep ocean voyage. The gills, mantles,...
1. Myoblast proliferation and differentiation is one of the most important biological processes in development skeletal muscle. MicroRNAs (miRNAs) play a crucial role this process.2. In study, expression level miR-181a-5p was detected, which found that expressed differently different tissues, embryonic ages, stages primary myoblasts Gushi chickens.3. The effect further investigated on chicken (CPMs). results cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU) cycle showed could...
Tissue factor pathway inhibitor 2 (TFPI2) plays a key role in female reproduction. However, its expression and function chickens are still unclear. In the present study, ovarian tissues from 30 w 15 laying were analyzed using RNA-seq to identify differentially expressed gene TFPI2. The full-length cDNA of TFPI2 was obtained adult chicken ovaries by rapid-amplification ends (RACE), putative protein found share highly conserved amino acid sequence with known bird homologs. addition, widely...
Abstract Air gun arrays are designed to generate strong energy pulses of less than 100 Hz for offshore oil and gas exploration. In shallow seas, due the directivity air arrays, complex propagation conditions limitations acquisition methods, it is difficult accurately evaluate spatial time frequency transmission characteristics airguns. To alleviate existing lack knowledge about in waters Bohai Sea, we analysed three‐dimensional ocean bottom cable seismic data collected during a survey China,...
Abstract Background Metabolic syndrome(MetS) and depression are independently associated with type 2 diabetes (T2DM) risk. However, little is known about the combined effect of MetS on risk T2DM. The present study aims to prospectively explore together T2DM susceptibility among Chinese general population. Methods 6489 population without adults in Southwest China were recruited from 2010 2012. Depression assessed by using 9-item Patient Health Questionnaire guidelines for prevention treatment...