Abstract Long noncoding RNAs (lncRNAs) are essential regulators of gene expression and biological behaviors. However, the contribution lncRNA LUCAT1 to choroidal melanoma (CM) remains unexplored. Here, we examined in CM cells by qRT‐PCR investigated its effects cell counting kit‐8, EdU, TUNEL, transwell assays, Western blot. Bioinformatics tools were applied find RNA candidates for further study. Moreover, mechanistic experiments including immunoprecipitation assay, pull‐down luciferase...

Melanoma is a malignant tumor derived from melanocytes, which the most fatal skin cancer. The present study aimed to explore and elucidate candidate genes in melanoma its underlying molecular mechanism. A total of 1,156 differentially expressed were obtained GSE46517 dataset Gene Expression Omnibus database using package "limma" R. Based on two algorithms (LASSO SVM-RFE), we three DEGs (LTBP4, CDHR1, MARCKSL1). Among them, LTBP4 was identified as diagnostic marker (AUC = 0.985)....

The aims of this study were to investigate 21 cases transcranial orbitotomy for cranio-orbital tumors and evaluate the clinical value surgical approach.A retrospective was done on patients with tumors. According tumors' location, shape, boundary, invasion images, coronal scalp flap approach, subfrontal or pterion approach performed.The route in 7 (including 3 combined lateral orbitotomy), 6 cases, 8 1 case evisceration orbit). Tumors en bloc resection 4 block curettage 13 incomplete cases....

To evaluate the ophthalmic manifestations and radiographic features of ectopic orbital meningioma to improve diagnostic accuracy. Patient data from patients admitted our institution during a 217-month period August 1999 September 2017 were included. manifestations, (CT MRI), diagnosis, pathology, therapeutic regimens, prognosis retrospectively reviewed. Six with identified. The mean age at first visit was 33.2 years (range, 7–56 years). All six displayed exophthalmos, upper eyelid oedema,...

Objective. To explore the clinical manifestations, imaging characteristics, and pathological characteristics of Castleman’s disease lacrimal gland, enhance knowledge disease, improve level its diagnosis treatment. Methods. In retrospective study, data 5 patients diagnosed with gland in Tianjin Medical University Eye Hospital from 2014 to 2018 were analyzed, relevant literature was reviewed. Results. All confirmed by examination. Clinical manifestations characterized mass occupying lesions...

Purpose Chemoresistance remains the primary obstacle threatening prognosis of retinoblastoma (RB). microRNAs (miRNAs) are acknowledged as critical regulators drug resistance. This study explored molecular mechanism miR-130a-3p affecting chemosensitivity RB to vincristine (VCR).Methods expression human retinal astrocytes and cell lines (Y79, WERI-Rb-1, SO-Rb50, SO-Rb70) was detected using RT-qPCR. VCR-resistant line Y79/VCR induced. its corresponding parental detected. cells were subjected...

We aimed to evaluate the genetic variation of poly (ADP-ribose) polymerase-1 (PARP-1) as risk factor in development Graves' disease (GD) and ophthalmopathy (GO) among Chinese individuals. Patients with confirmed diagnosis GD or healthy individuals no clinical symptoms hyperthyroiditis were enrolled at Department Ophthalmology, Tianjin First Center Hospital, China. Genetic polymorphism was studied plasma DNA samples subjects by polymerase chain reaction restriction fragment length confirm our...

Orbital venous malformation results from the aberrant angiogenesis in orbit; however, detailed molecular mechanism is still not clear. In this study, tissue samples 27 patients with orbital were collected and subjected to whole exome sequencing. Melanocortin 4 receptor was gene highest incidence (7/27) of mutation identified series. A total four types mutations found coding region 5ʹ-untranslated melanocortin receptor. All these resulted upregulation expression. vitro assays using human...

With development of the modern medical imaging technology,CT,MRI,ultrasound and OCT,etc,have been widely used in clinical ophthalmology improve level diagnosis treatment eye diseases.Ultrasound has a large diagnostic application for intraocular disease.CT is preferred method orbital disease,with advantage clearly showing normal structure orbit,especially bone changes.MRI images specificity ocular tumor,such as choroidal melanoma,it also effective searching range intracranial lesions by optic...

Abstract Purpose Chemoresistance remains the primary obstacle threatening prognosis of retinoblastoma (RB). microRNAs (miRNAs) are acknowledged as critical regulator drug resistance. This study explored molecular mechanism miR-130a-3p affecting chemosensitivity RB to vincristine (VCR). Methods expression VCR-sensitive and VCR-resistant tissues was detected using RT-qPCR. cell line Y79/VCR induced. its corresponding parental detected. cells were subjected overexpression treatment. The...

Objective To explore the recurrence and poor prognosis mechanism from perspective multi-drug resistance (MDR) by detecting expression of P-gp,GST-π LRP in orbital lymphoma.Methods Fifty lymphoma tissue which were diagnosed pathology after surgery collected Tianjin eye hospital first centre hospital.Among them,46 cases observation group.Meanwhile,17 reactive lymphoid hyperplasia tissues as contrast group.Orbital microarray immunohistochemical staining was used to detect hyperplasia.Results...

Image segmentation was adopted to process and analyze diabetic retinopathy vasculars so as improve the efficiency precision of clinical diagnosis. By means selecting fundus images research object appling computer simulation software, retinal vessels were divided extracted. After normal pathological image processed analysised, more fully revealed, precise than fluorescein angiography(FFA). Processing analyzing based on are detail clear, has been significantly improved.

Selection of clinical image the fundus and fluoresce in angiography (fundus angiography, FFA) as research object, using segmentation method on diabetic retinopathy (Diabetic Retinopathy, DR) vascular processing analysis, order to improve diagnostic accuracy efficiency. The experimental results show that analysis methods for normal retinal images are relatively complete lesions (Fundus Fluoresce Angiography compared more detailed clear, precision is improved obviously. As eye blood vessel...

ObJective To study the hemodynamic characteristics of ophthalmic artery and central retinal in patients with thyroid-associated ophthalmopathy (TAO),and to analyze relevance between blood flow parameters clinical characteristics.At last,discuss different influence factors on supply TAO.Methods Use U.S.company GE's Logic7 Color Doppler Ultrasound diagnostic apparatus,to detect 49 TAO cases (90 eyes) whose thyroid function was normal or stable over six months 24 controls (48...

Objective To analyze the change of tear film after phacoemulsification in patient with renal transplantation and discuss problem dry eye.Methods A non-randomized cases-controlled study was designed.26 cataract patients (the experimental group) (34 eyes) matched 33 (40 age-related cataract(control were included this study.Before different time IOL implantation respectively performed on all patients,dry eye-related symptom surveyed scored by questionnaire,and break-up (BUT),Schirmer I test...

Objective To present some reference for accurate diagnosis and treatment of orbital space-occupying lesions in clinic,and to analyze the pathological classification evaluate frequencies various types lesions.Methods In this retrospective case series,the medical records 165 patients referred Tianjin first center hospital between November 2009 2012 were reviewed.Results Benign tumors found 98 eyes (59.39％),the leading 3 cavernous hemangioma,21 (21.43％); inflammatory pseudotumor,19 (19.39％);...

Objective To study CT imaging features of orbital blow-out fractures.Methods Retrospective and analysis was made on the clinical records 73 fracture patients from September 2009 to October 2011.Sixty-two cases with treated by surgical treatments 14 conservative were reviewed summarized.The condition in diplopia,enophthalmos degree,eyeball position detected.The quality,position,and scope fractures change cavity must be noticed,and understand relation between tissues or extraorcular muscles...

Objective To investigate the value of MRI on lacrmal gland lesions.Methods The findings lacrimal lesions in 36 cases were analyzed retrospectively and main outcome measures anatomic extent,configuration,margins,internal features T1 T2 weighted images,contrast enhancement adjacent bone change MRI.Results Of patients,4 had dacryoadenitis,9 inflammatory pseudotumor,8 lymphoid tumors,7 pleomorphic adenoma,and 8 adenoid cystic carcinoma.The results patients with chronic dacryoadenitis...

Objective To investigate the imaging findings of paranasal sinus communication eye lesions.Methods Forty cases lesions were retrospectively analyzed.CT performed in all cases; X-ray was 6 MRI 7cases.All confirmed by operation and pathologic examination.Results The showed to originated from maxillary 10 cases,from ethmoidal 21cases,from frontal 7cases,from sphenoidal 2cases.31 with benign 9 malignant disease.Orbital CT scan smooth clear borders,homogeneous density,and dilatability bone...