A5053417146- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Circular RNAs in diseases
- Adenosine and Purinergic Signaling
- Pulmonary Hypertension Research and Treatments
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cystic Fibrosis Research Advances
- Genetics and Neurodevelopmental Disorders
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Reproductive System and Pregnancy
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Macrophage Migration Inhibitory Factor
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Retinal Diseases and Treatments
- Cardiac Fibrosis and Remodeling
- Nuclear Receptors and Signaling
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Rare Diseases
- Ectopic Pregnancy Diagnosis and Management
Nanjing Maternity and Child Health Care Hospital
2018-2025
Nanjing Medical University
2016-2025
Wuxi People's Hospital
2016-2023
Guangdong University Of Finances and Economics
2023
Guangdong University of Finance
2023
Guizhou Provincial People's Hospital
2023
HBIS (China)
2022
Hebei Agricultural University
2012-2022
Nanjing University of Chinese Medicine
2020
Jiangsu Province Hospital
2020
Retinal pigment epithelium (RPE) cell damage is implicated in the pathogenesis of age-related macular degeneration (AMD). An increase interferon-γ (IFN-γ) levels was observed patients with AMD, but whether inflammatory factors are causally related to AMD progression unclear. Here, we demonstrate a direct causal relationship between IFN-γ and RPE death. induced human retinal epithelial (ARPE-19) death accompanied by increases Fe2+ , reactive oxygen species, lipid peroxidation, glutathione...
Epidemiological studies have shown that elevated concentrations of ambient particulate matter (aerodynamic diameter ≤2.5 μm; PM2.5) correlates with increased incidence asthma. The aim this study was to determine whether PM2.5 participates in the exacerbation asthma.Effects 1, 10 and 100 μg instilled intratracheally ovalbumin (OVA)-sensitized or asthmatic mice were compared.PM2.5 exposure OVA-sensitized especially groups Mch responsiveness a dose-dependent manner. In groups, 1 caused no...
POSTN (Periostin) is an ECM (extracellular matrix) protein involved in tissue remodeling response to injury and a contributing factor tumorigenesis, suggesting that plays role the pathogenesis of pulmonary hypertension (PH).We aimed gain insight into mechanistic contribution experimental mouse models PH correlate these findings with humans.We used genetic epistasis approaches human artery endothelial cells (hPAECs), smooth muscle cells, (Sugen 5416/hypoxia or chronic hypoxia) discern its...
Particulate matter 2.5 (PM2.5) is a significant risk factor for asthma. A recent study revealed that autophagy was associated with asthma pathogenesis. However, the specific mechanisms underlying PM2.5‑induced in have remained elusive. In present study, evaluated Beas‑2B human bronchial epithelial cells and potential molecular were investigated. Using electron microscopy, immunofluorescence staining immunoblot studies, it confirmed PM2.5 induced as result of PM2.5‑mediated inhibition...
Diabetic retinopathy (DR) is a common microvascular complication of diabetes mellitus. Abnormal energy metabolism in endothelium involved the progression diabetic retinopathy. Bile Acid G-Protein-Coupled Membrane Receptor (TGR5) has emerged as novel regulator metabolic disorders. However, role TGR5 mellitus-induced dysfunction retinas largely unknown. Herein, enzyme-linked immunosorbent assay was used for analyzing bile acid (BA) profiles rat and retinal endothelial cells (RMECs) cultured...
Abstract Spinal muscular atrophy (SMA) is an autosomal recessive and often fatal neurological disease. However, very little known about the attitudes toward SMA carrier screening among Chinese pregnant people. In this study, women in Eastern China who were undergoing routine chromosomal programs invited to view educational video complete a 26‐item survey regarding their by scanning specific quick response code. A total of 1673 questionnaires collected, 81.1% respondents willing undergo...
Chromosomal inversions are underappreciated causes of rare diseases given their detection, resolution, and clinical interpretation remain challenging. Heterozygous mutations in the MEIS2 gene cause an autosomal dominant syndrome characterized by intellectual disability, cleft palate, congenital heart defect, facial dysmorphism at variable severity penetrance. Herein, we report a Chinese girl with developmental delay, whom G-banded karyotype analysis identified de novo paracentric inversion...
Asthma is a common chronic respiratory disease. In previous study, we found several circulating microRNA signatures associated with childhood asthma and selected miR-3162-3p for subsequent studies. Since the target proteins underlying molecular mechanisms of in etiopathogenesis are not well characterized, designed this study to clarify its role. We employed bioinformatics quantitative PCR methods as first step determine miR-3162-3p, elucidated β-catenin. Luciferase assays western blot...
MicroRNAs (miRNAs) exist stably and reproducibly in plasma may be used as biomarkers for various diseases. Little is known about circulating miRNAs the peripheral blood of juvenile patients with asthma.In this study, we hybridization arrays to compare miRNA expression profiles among 6 or without asthma. Using quantitative PCR (qPCR), verified levels these from asthma (n = 40) healthy subjects 14).Our results showed that miR-Let7C, miR-486, miR-1260a childhood were significantly higher than...
Abstract Solute carrier family 12 member 5 (SLC12A5), an integral membrane KCl cotransporter, which maintains chloride homeostasis in neurons, is aberrantly expressed and involved the tumorigenesis of certain cancers. However, clinical significance biological role SLC12A5 human bladder urothelial carcinoma (BUC) remains unclear. In this study, expression was examined specimens primary BUC cell lines using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR),...
Abstract Background Next‐generation sequencing has been invaluable to delineate the genetic etiology of neurodevelopmental disorders (NDDs) in recent years. BCL11B , encoding Cys 2 His zinc finger transcription factor, is essential for development immune and neural systems. Methods Herein, we describe a Chinese girl presenting craniofacial abnormalities, developmental delay intellectual disability with speech impairment. Exomes genes were enriched Agilent SureSelect QXT ALL Human Exon V6 kit...
Hyperphosphorylation of tau is pivotally involved in the pathogenesis Alzheimer’s disease (AD) and related tauopathies. Glycogen synthase kinase-3β (GSK-3β) protein phosphate 2A (PP2A) are crucial enzymes to regulate phosphorylation. GSK-3β activity regulated by its inhibitory phosphorylation at Ser9. We previously reported cross-talk between PP2A signaling showed that could dephosphorylate Here, we investigated dephosphorylation brain extracts presence phosphatase inhibitors found a...
Microdeletions in Y-chromosomal azoospermia factor (AZF) regions have been regarded as the risk of spermatogenic failure (SF). However, AZF-linked duplications or complex copy number variants (CNVs) (deletion + duplication) were rarely studied. In this study, we performed multiplex ligation-dependent probe amplification (MLPA) analysis on 402 fertile healthy male controls and 423 idiopathic infertile SF patients (197 226 oligozoospermia) Han Chinese population. total, twenty-four types CNVs...
Age-related thymic involution is characterized by a loss of epithelial cells (TECs) and concomitant increase in adipocytes, but the mechanisms involved adipogenesis are still not clear. Transforming growth factor β1 (TGF-β1) pleiotropic cytokine that has been reported to be up-regulated with age stromal both human mouse. However, exact role TGF-β1 age-related remains further elucidated. On basis previous findings, we propose novel hypothesis functions dual involution. one hand, up-regulation...
The effects of the expression a small heat shock protein (shsp) gene from Streptococcus thermophilus on stress resistance in Lactococcus lactis under different environmental stresses were investigated this study. pMG36e-shsp, an vector, was first constructed by inserting shsp open reading frame (ORF) cloned S. strain St-QC into pMG36e. Then, food-grade pMG-shsp, generated deleting erythromycin pMG36e-shsp. transformation rate pMG-shsp comparable to that pMG36e-shsp when each these two...