A5018560293- RNA regulation and disease
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- RNA and protein synthesis mechanisms
- interferon and immune responses
- Protein Structure and Dynamics
- Chromatin Remodeling and Cancer
- Hereditary Neurological Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Winter Sports Injuries and Performance
- CAR-T cell therapy research
- Viral Infections and Immunology Research
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Phagocytosis and Immune Regulation
- Bacterial Genetics and Biotechnology
- Sunflower and Safflower Cultivation
- Peptidase Inhibition and Analysis
- Landslides and related hazards
- Collembola Taxonomy and Ecology Studies
- Genomics and Phylogenetic Studies
University of Michigan
2021-2024
Dana-Farber Cancer Institute
2019-2022
Harvard University
2019-2022
Complex neural circuitry requires stable connections formed by lengthy axons. To maintain these functional circuits, fast transport delivers RNAs to distal axons where they undergo local translation. However, the mechanism that enables long-distance of RNA granules is not yet understood. Here, we demonstrate a complex containing and RNA-binding protein (RBP) SFPQ interacts selectively with tetrameric kinesin adaptor KLC1 motor KIF5A. We show binding KIF5A/KLC1 required for axon survival...
Dysregulated Wnt/β-catenin signaling is implicated in the pathogenesis of many human cancers, including colorectal cancer (CRC), making it an attractive clinical target. With aim inhibiting oncogenic Wnt activity, we developed a high-throughput screening AlphaScreen assay to identify selective small-molecule inhibitors interaction between β-catenin and its coactivator BCL9. We identified compound that consistently bound specifically inhibited vivo native β-catenin/BCL9 complex formation CRC...
Detecting viral infection is a key role of the innate immune system. The genomes some RNA viruses have high CpG dinucleotide content relative to most vertebrate cell RNAs, making CpGs molecular marker infection. human zinc-finger antiviral protein (ZAP) recognizes CpG, mediates clearance foreign CpG-rich RNA, and causes attenuation viruses. While ZAP binds it lacks enzymatic activity that might be responsible for degradation thus requires interacting cofactors its function. One these...
ZAP is an antiviral protein that binds to and depletes viral RNA, which often distinguished from vertebrate host RNA by its elevated CpG content. Two cofactors, TRIM25 KHNYN, have activities are poorly understood. Here, we show functional interactions between ZAP, KHNYN involve multiple domains of each protein, the ability multimerize via RING domain augments activity specificity. We active nuclease acts in a partly redundant manner with homolog N4BP1. The N-terminal binding constitutes...
Abstract Complex neural circuitry requires stable connections formed by lengthy axons. To maintain these functional circuits, fast transport delivers RNAs to distal axons where they undergo local translation. However, the mechanism that enables long distance of non-membrane enclosed organelles such as RNA granules is not known. Here we demonstrate a complex containing and RNA-binding protein (RBP) SFPQ interacts directly with tetrameric kinesin adaptor KLC1 motor KIF5A. We show binding...
ABSTRACT Enzymatic pockets such as those of histone deacetylases (HDACs) are among the most favored targets for drug development. However, enzymatic inhibitors often exhibit low selectivity and high toxicity due to targeting multiple enzyme paralogs, which involved in distinct multisubunit complexes. Here, we report discovery characterization a non-enzymatic small molecule inhibitor HDAC transcriptional repression functions with comparable anti-tumor activity Vorinostat, anti-psychedelic an...
Abstract RNAs play myriad functional and regulatory roles in the cell. Despite their significance, three-dimensional structure elucidation of RNA molecules lags significantly behind that proteins. NMR-based studies are often rate-limited by assignment chemical shifts. Automation shift process can greatly facilitate structural studies, however, accurate predictions rely on a robust complete database for training. We searched Biological Magnetic Resonance Data Bank (BMRB) to identify sequences...