A5101932665- Proteoglycans and glycosaminoglycans research
- Pancreatic and Hepatic Oncology Research
- Fibroblast Growth Factor Research
- Glycosylation and Glycoproteins Research
- Gallbladder and Bile Duct Disorders
- MicroRNA in disease regulation
- Pancreatitis Pathology and Treatment
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Minimally Invasive Surgical Techniques
- Circular RNAs in diseases
- Gastric Cancer Management and Outcomes
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- DNA Repair Mechanisms
- Appendicitis Diagnosis and Management
- Microtubule and mitosis dynamics
- Colorectal Cancer Surgical Treatments
- Cancer-related molecular mechanisms research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Peptidase Inhibition and Analysis
- Cell death mechanisms and regulation
University of Occupational and Environmental Health Japan
2015-2024
Kyushu University
1999-2023
Takaoka University of Law
2021
Kyushu Rosai Hospital
2021
Kyushu University Hospital
2009
Cancer Research Center
2005
Johns Hopkins University
2004
Yamagata University
1991
Quantitative proteomics can be used as a screening tool for identification of differentially expressed proteins potential biomarkers cancers. Candidate from such studies subsequently tested using other techniques use in early detection Here we demonstrate the stable isotope labeling with amino acids cell culture (SILAC) method to compare secreted (secretome) pancreatic cancer-derived cells that non-neoplastic ductal cells. We identified 145 (>1.5-fold change), several which were previously...
Due to the poor prognosis of pancreatic cancer, novel diagnostic modalities for early diagnosis and new therapeutic strategy are urgently needed. Recently, microRNA-21 (miR-21) was reported be strongly overexpressed in cancer as well other solid cancers. We investigated functional roles miR-21, which have not been fully elucidated cancer. miR-21 expression assessed cell lines (14 lines, primary cultures normal epithelial cells fibroblasts, a human ductal line) tissue samples (25 tissues 25...
Abstract Background Recently, the microRNA-200 family was reported to affect cancer biology by regulating epithelial mesenchymal transition (EMT). Especially, expression of miR-200c has been shown be associated with upregulating E-cadherin , a gene known involved in pancreatic behavior. However, significance is unknown. Methods In present study, we investigated relationship between and panel 14 cell lines macro-dissected formalin-fixed paraffin-embedded (FFPE) tissue samples obtained from 99...
Abstract Background Inflammation plays a multifaceted role in cancer progression, and NF‐κB is one of the key factors connecting inflammation with progression. We have shown that lipopolysaccharide (LPS) promotes activation colon cells pancreatic cells. However, it unclear why inflammatory stimuli can induce Methods used two human cells, Panc‐1 AsPC‐1, as target LPS was an stimulus. To confirm participation TLR4/NF‐κB signaling pathway, we three different inhibitors (PDTC, IκBα mutant, decoy...
Although pancreatic ductal adenocarcinoma is characterized by an abundant stroma enriched with hyaluronan (HA), the prognostic impact of HA and its regulators remains unknown.Using immunohistochemistry, expression patterns regulators, including a synthesizing enzyme (HAS2), degrading (HYAL1) were investigated in patients who received surgical resection. The significance these markers other clinicopathological variables was determined using univariate multivariate analyses. levels...
The interactions between cancer cells and surrounding stroma play a critical role in tumor progression, but their molecular basis is largely unknown. Global gene expression profiling was performed using oligonucleotide microarrays to determine changes the of pancreatic (CFPAC1) stromal fibroblasts induced by coculture. This analysis identified multiple genes as differentially expressed consequence mutual interactions, including those that encode for proteins associated with invasion,...
To evaluate the risk factors for pancreatic fistula after head resection.Retrospective review.University hospital, in 71-month period from January 1992 through November 1997.Sixty-two patients who underwent resection with pancreatojejunostomy. We performed an extensive analysis of preoperative and perioperative fistula.Pancreatic was defined as high amylase level (> 1000 U/L) drainage fluid collected peripancreatic drains and/or anastomotic disruption demonstrated radiographically.Nine (15%)...
Abstract BACKGROUND: Recently, cancer stem cells have been reported as a new therapeutic target in pancreatic well other cancers, but the specific role of these is unknown. METHODS: The authors investigated functional roles CD133 + cells, 1 putative cell candidates cancer. expression was assessed human and lines by quantitative real‐time reverse transcriptase polymerase chain reaction flow cytometry. Next, they compared ability − to proliferate, migrate, invade using 2 lines. In particular,...
// Xiao-Bo Cheng 1, 2 , Shiro Kohi 1 Atsuhiro Koga Keiji Hirata Norihiro Sato Department of Surgery School Medicine, University Occupational and Environmental Health, Kitakyushu, Fukuoka 807-8555, Japan Breast Surgery, The Fourth Affiliated Hospital China Medical University, Shenyang, Liaoning 110032, Correspondence to: Cheng, e-mail: c13840564883@163.com & chengxiaobo@med.uoeh-u.ac.jp Sato, norisato@med.uoeh-u.ac.jp Keywords: hyaluronan, pancreatic cancer, migration, hyaluronan synthase...
Receptor for hyaluronic acid (HA)-mediated motility (RHAMM) is a nonintegral cell surface receptor involved in the aggressive phenotype wide spectrum of human malignancies, but significance RHAMM pancreatic ductal adenocarcinoma (PDAC) remains unknown. In this study, we investigated expression and its clinical relevance PDAC. mRNA was examined 8 PDAC lines primary cancer adjacent non-tumor tissues from 14 patients using real-time RT-PCR. Western blotting carried out to analyze protein lines....
Recent evidence suggests a critical role of hyaluronan (HA), especially low-molecular-weight HA (LMW-HA), in the aggressive tumor phenotype. Increased expression KIAA1199, newly identified protein involved degradation, has been reported various cancers, including pancreatic ductal adenocarcinoma (PDAC). However, little is known about functional significance KIAA1199 PDAC.Using siRNA knockdown and forced models, we investigated effects on malignant behaviors (proliferation, migration,...
Pancreatic ductal adenocarcinoma (PDAC) is characterized by accelerated production and degradation of hyaluronan (HA), a major component extracellular matrix involved in the malignant phenotype cancer. In particular, increased hyaluronidase (HYAL) activity plays critical role cancer progression, at least part, producing low-molecular-weight- (LMW-) HA or small fragments HA, suggesting HYAL as target for treatment. Hyaluromycin, new member rubromycin family antibiotics, was isolated from...
BACKGROUND Despite the recent clinical finding that high telomerase activity is an unfavorable prognostic marker for various human malignant tumors, there has been no experimental evidence supporting link between and tumor aggressiveness. In current investigation, authors examined relation potential biologic aggressiveness in pancreatic carcinoma cells. METHODS Telomerase was measured a poorly metastatic cell line HPC-3 its highly variant HPC-3H4, as well many lines. Aggressive behavior of...