A5034265370- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Animal Nutrition and Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hypothalamic control of reproductive hormones
- Reproductive biology and impacts on aquatic species
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Animal Virus Infections Studies
- Livestock and Poultry Management
- Reproductive Physiology in Livestock
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Electrospun Nanofibers in Biomedical Applications
- Plant Reproductive Biology
- Animal Behavior and Reproduction
- Plant Genetic and Mutation Studies
- Cardiac Valve Diseases and Treatments
- Pancreatitis Pathology and Treatment
- Transplantation: Methods and Outcomes
- Pancreatic function and diabetes
- Trauma Management and Diagnosis
- Gastrointestinal disorders and treatments
- Infectious Aortic and Vascular Conditions
Pennsylvania State University
2025
Beltsville Agricultural Research Center
2011-2024
Agricultural Research Service
2011-2024
United States Department of Agriculture
2008-2024
Eastern Michigan University
2022
North Carolina State University
2020
Joe DiMaggio Children's Hospital
2015-2019
Stevenson University
2016
Sir Charles Gairdner Hospital
2004
The University of Western Australia
2002-2004
A synergistic combination of two next-generation sequencing platforms with a detailed comparative BAC physical contig map provided cost-effective assembly the genome sequence domestic turkey (Meleagris gallopavo). Heterozygosity sequenced source allowed discovery more than 600,000 high quality single nucleotide variants. Despite this heterozygosity, current (∼1.1 Gb) includes 917 Mb assigned to specific chromosomes. Annotation identified nearly 16,000 genes, 15,093 recognized as protein...
The value of the ability to cryopreserve and store germplasm has long been recognized for indefinite preservation genetic material, especially at-risk populations. In contrast domestic livestock species, cryogenic storage poultry semen is not reliable enough preservation. relatively low fertilizing frozen/thawed sperm most likely results from physiological sensitivity process coupled with requirement prolonged functionality in hen reproductive tract; however, concept defining these...
The turkey (Meleagris gallopavo) is an important agricultural species and the second largest contributor to world's poultry meat production. Genetic improvement attributed largely selective breeding programs that rely on highly heritable phenotypic traits, such as body size breast muscle development. Commercial with small effective population sizes epistasis can result in loss of genetic diversity, which turn lead reduced individual fitness response selection. presence genomic diversity...
Pancreatitis in children is not common but can be associated with severe morbidity rates. We have treated 49 pancreatitis over the past 12 years ranging age from 1 month to 18 years. One third of patients had biliary tract disease as an etiology, nearly half these being related underlying hematologic disease, usually sickle cell anemia. Another was due trauma, and one were child abuse. Other etologies systemic (6 patients), congenital anomalies (8 idiopathic (3 cases). Eighty-two per cent...
ABSTRACT: Limited reproductive data are available for any species of howler monkey, including those listed as threatened ( Alouatta pigra ) and endangered A palliata by the Convention on International Trade in Endangered Species Status (CITES) report. The Southern black monkey caraya is being considered a model to develop assisted technology (ART) vulnerable species. Specific objectives this study were evaluate effect 1) time year ejaculate quality testosterone concentration, 2) age male...
The efficiency of sperm capacitation and the acrosome reaction was studied in teratospermic domestic cat to evaluate further etiology compromised zona pellucida penetration oocyte fertilization. Specific objectives were compare normospermic ejaculates for 1) kinetics timing vitro as determined by an ionophore-induced reaction; 2) incidence spontaneous acrosomal loss; 3) ability capacitated, swim-up processed acrosome-react response chemical (calcium ionophore) or physiological (solubilized...
Semen cryopreservation is necessary for banking germplasm from critical poultry stocks. To date, glycerol the most effective cryoprotectant sperm; however, contraceptive effects of require a significant reduction thawed semen before artificial insemination (AI). The effectiveness by dialysis, Percoll density gradient centrifugation, or washing through 12% (wt/vol) Accudenz was evaluated fertility trials with highly inbred chicken research lines and commercial turkey lines. extended 1:1 then...
The fertility rates of cryopreserved poultry semen are highly variable and not reliable for use in preservation commercial genetic stocks. Our objective was to evaluate the cryosurvival from 8 pedigreed layer lines at 2 different ages: onset end production. Semen 160 roosters (20/line) frozen individually with 11% glycerol 6 12 mo age. Glycerol removed thawed by Accudenz gradient centrifugation. viability sperm each male determined using fluorescent live-dead staining flow cytometry;...
A preovulatory surge (PS) of luteinizing hormone (LH) and progesterone triggers follicle ovulation, which is the first step egg production orchestrated by hypothalamo-pituitary-gonadal (HPG) axis. In HPG axis, hypothalamic peptides, gonadotropin releasing hormone, inhibitory control stimulating LH pituitary, subsequently regulate ovarian estradiol progesterone, respectively. The goal this study was to characterize axis function average producing hens assessing plasma profiles hypothalamic,...
Variation in egg production exists commercial turkey hens, with low producing hens (LEPH) costing more per produced than high (HEPH). Egg correlates ovulation frequency, which is governed by the hypothalamic-pituitary-gonadal (HPG) axis. Ovulation stimulated a preovulatory surge (PS) of progesterone and luteinizing hormone, triggered gonadotropin releasing hormone release inhibited inhibiting hormone. Differences between LEPH HEPH were characterized determining HPG axis plasma profiles mRNA...
Turkey sperm lose viability within 8-18 h when stored as liquid semen using current methods and extenders. In contrast, turkey hens maintain viable, fertile in their storage tubules (SST) for 45 or more days following a single insemination. Our long-term objectives are to identify characterize differentially expressed genes that may underlie this prolonged then use information develop improved storing semen. We employed serial analysis of gene expression (SAGE) compare patterns SST recovered...
Sperm storage tubules (SST) are specialized invaginations of the oviductal epithelium that permit avian species to store spermatozoa for extended periods time, without compromising sperm fertilization capacity. The molecular and physiological mechanisms behind tubule differentiation, protection, regression remain largely unknown, but most likely have potential implications substantially improving hen fertility, storage, semen cryopreservation in commercial poultry species. RNA sequencing was...
Sperm storage within the uterovaginal junction (UVJ) of avian species occurs in specialized structures termed sperm tubules (SSTs) and allows for prolonged semen, though molecular mechanisms involved semen preservation are not well understood. Little work has been done examining how function SSTs is impacted by insemination present SSTs.
The effects of gonadotropin treatment and laparoscopic artificial insemination (AI) on embryo quality, serum progesterone estradiol concentrations, luteal content were examined in the domestic cat. These data compared to similar historical reported for naturally estrual, mated queens. All queens this study (n = 32) treated with eCG followed by 1) natural breeding (eCG-NB), 2) NB hCG (eCG-NB-hCG), 3) a sham AI procedure (eCG-NB-sham AI), or 4) actual (eCG-hCG-AI). Queens ovulating response...
Protein tyrosine phosphorylation is associated with sperm capacitation and the acrosome reaction in several mammalian species. Changes of a 95-kDa protein human, mouse, domestic cat spermatozoa are known to be influenced by exposure zona pellucida (ZP) proteins. We previously reported diminished 95- 160-kDa proteins from teratospermic cats, compared normospermic cats. To determine if these mechanisms present other species phenotypically diverse Felidae family, we examined relationship...