A5002573985- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Trace Elements in Health
- Gut microbiota and health
- Smoking Behavior and Cessation
- Drug Transport and Resistance Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Genetic Syndromes and Imprinting
- Salivary Gland Disorders and Functions
- Cancer-related gene regulation
- Air Quality and Health Impacts
- Cancer-related molecular mechanisms research
- Pediatric health and respiratory diseases
- Peroxisome Proliferator-Activated Receptors
- Nutrition, Genetics, and Disease
- Pharmacological Effects and Toxicity Studies
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Birth, Development, and Health
- Nutritional Studies and Diet
- Health, Environment, Cognitive Aging
- Regulation of Appetite and Obesity
- Cardiovascular Health and Risk Factors
- Iron Metabolism and Disorders
- Inflammatory mediators and NSAID effects
The Ohio State University
2016-2025
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2014-2023
Ohio University
2018-2020
University of Hawaiʻi at Mānoa
2011-2016
University of Hawaii System
2011-2016
University of Hawaii Cancer Center
2011-2016
Cancer Center of Hawaii
2011-2016
Sylvester Comprehensive Cancer Center
2016
Comprehensive Blood & Cancer Center
2015
Korea University of Science and Technology
2015
The causative agents for the current national outbreak of electronic-cigarette, or vaping, product use-associated lung injury (EVALI) have not been established. Detection toxicants in bronchoalveolar-lavage (BAL) fluid from patients with EVALI can provide direct information on exposure within lung.BAL fluids were collected 51 16 states and 99 healthy participants who part an ongoing study smoking involving nonsmokers, exclusive users e-cigarettes vaping products, cigarette smokers that was...
Background Hepatocellular carcinoma (HCC) is one of the most common cancers and frequently presents with an advanced disease at diagnosis. There only limited knowledge genome-scale methylation changes in HCC. Methods Findings We performed genome-wide profiling a total 47 samples including 27 HCC 20 adjacent normal liver tissues using Illumina HumanMethylation450 BeadChip. focused on differential patterns promoter CpG islands as well various less studied genomic regions such those surrounding...
Abstract Electronic cigarette (e-cig) use is continuing to increase, particularly among youth never-smokers, and used by some smokers quit. The acute chronic toxicity of e-cig unclear generally in the context increasing reports inflammatory-type pneumonia users. To assess lung effects e-cigs without nicotine or flavors, we conducted a pilot study with serial bronchoscopies over 4 weeks 30 randomized either 4-week intervention containing only 50% propylene glycol (PG) vegetable glycerine...
Background Phosphorylase kinase (PhK), also known as adenosine triphosphate (ATP)-phosphorylase b phosphotransferase, integrates multiple calcium/calmodulin-dependent signalling pathways, including those involved in cell migration and proliferation, while coupling these pathways to glycogenolysis ATP-dependent phosphorylation, thus ensuring continuing energy supply for activities. Objectives Our laboratory recently reported correlation of elevated PhK activity with psoriatic activity. This...
Nicotine-containing electronic cigarette (e-cig) use has become widespread. However, understanding the biological impact of e-cigs compared with smoking on lung is needed. There are major gaps in knowledge for chronic effects and an etiology to recent acute toxicity leading death among vapers.We conducted bronchoscopies a cross-sectional study 73 subjects (42 never-smokers, 15 e-cig users, 16 smokers). Using bronchoalveolar lavage brushings, we examined inflammation by cell counts,...
One of the responses to administration thyroid hormone is an increase in malic enzyme (EC 1.1.1.40)mRNA rat liver.We have previously shown that 3,5,3*-triiodo-~-thyronine (Ts) causes a 3-4-fold rate transcription gene as determined by vitro run-off assays with cDNA probe following T3 treatment for 10 days (Dozin, ' V. M. Nikodem, manuscript preparation.
Pre-eclampsia is the leading cause of fetal and maternal morbidity mortality. Early onset pre-eclampsia (EOPE) a disorder that has severe outcomes, whilst its etiology poorly understood. We hypothesize epigenetics plays an important role to mediate development EOPE conducted case–control study compare genome-wide methylome difference between chorioamniotic membranes from 30 17 full-term pregnancies using Infinium Human Methylation 450 BeadChip arrays. Bioinformatics analysis tested...
Breast cancer is more common in European Americans (EAs) than African (AAs) but mortality from breast higher among AAs. While there are racial differences DNA methylation and gene expression tumors, little known whether such exist tissues of healthy women. Genome-wide profiling was performed histologically normal Linear regression models were used to identify differentially-methylated CpG sites (CpGs) between EAs (n = 61) AAs 22). Correlations for assessed. Biological functions the genes...
Preeclampsia is one of the leading causes fetal and maternal morbidity mortality worldwide. Preterm babies mothers with early onset preeclampsia (EOPE) are at higher risks for various diseases later on in life, including cardiovascular diseases. We hypothesized that genome-wide epigenetic alterations occur cord blood DNAs association EOPE conducted a case control study to compare genome-scale methylome differences between 12 EOPE-associated 8 normal births.Bioinformatics analysis methylation...
BackgroundAn outbreak of E-cigarette or Vaping Product Use-Associated Lung Injury (EVALI) with significant morbidity and mortality was reported in 2019. While most patients EVALI report vaping tetrahydrocannabinol (THC) oils contaminated vitamin E acetate, a subset only nicotine-containing electronic cigarettes (e-cigs). Whether not e-cigs cause EVALI, the highlights need for identifying long term health effects e-cigs. pathology includes alveolar damage, pneumonitis and/or organizing...
The supernatant of cytoplasmic extract rat small intestine was mixed with 65Zn and subjected to Sephadex G-75 column chromatography. activity detected in four peaks estimated 800, 10,000, 50,000-75,000 mol wt, larger than 100,000 daltons. zinc-binding ligand from the 800 wt peak prostaglandin or prostaglandin-like as indicated by ultra-violet absorption spectrum, spectrum shift alcoholic potassium hydroxide, its effects on pulmonary vascular resistance, radioimmunoassay. Addition E2...
Abstract Purpose: To examine the effects of chemotherapy on circulating cell‐free DNA (cfDNA) composition in relation to investigational whole‐body measurement tumor activity by fluorine‐18 fluorocholine (FCH) positron emission tomography/computed tomography (PET/CT) hormone‐refractory prostate cancer (HRPC). Methods: Serial FCH PET/CT scans were performed eight patients with HRPC receiving docetaxel‐based chemotherapy. Corresponding serial cfDNA samples characterized microfluidic...
Abstract Background The pathogenesis of asthma and airway obstruction is the result an abnormal response to different environmental exposures. scientific premise our study was based on finding that FoxO1 expression increased in lung macrophages mice after allergen exposure human asthmatic patients. Macrophages are capable switching from one functional phenotype another, it important understand mechanisms involved transformation how their cellular function affects peribronchial stromal...
Despite known age-related DNA methylation (aDNAm) changes in breast tumors, little is about aDNAm normal tissues. Breast tissues from a cross-sectional study of 121 cancer-free women, were assayed for genome-wide methylation. mRNA expression was by microarray technology. Analysis covariance used to identify aDNAm's. Altered correlated with the corresponding gene and methyltransferase protein DNMT3A, immunohistochemistry. Publically-available TCGA-BRCA data replication. 1,214 aDNAm's...
Acute kidney injury (AKI) is a common clinical condition associated with diverse etiologies and abrupt loss of renal function. In patients sepsis, rhabdomyolysis, cancer, cardiovascular disorders, the underlying disease or therapeutic interventions can cause hypoxia, cytotoxicity, inflammatory insults to tubular epithelial cells (RTECs), resulting in onset AKI. To uncover stress-responsive disease-modifying genes, here we have carried out transcriptome profiling three distinct murine models...
Mitochondrial DNA copy number (mtCN) maintains cellular function and homeostasis, is linked to nuclear methylation gene expression. Increased mtCN in the blood associated with smoking respiratory disease, but has received little attention for target organ effects or electronic cigarette (EC) use.Bronchoscopy biospecimens from healthy EC users, smokers (SM), never-smokers (NS) were assessed associations of mtDNA point mutations, immune responses, expression using linear regression. Ingenuity...
The microbiome has increasingly been linked to cancer. Little is known about the lung and oral cavity microbiomes in smokers, even less for electronic cigarette (EC) users, compared with never-smokers. In a cross-sectional study (n = 28) of EC never-smokers, bronchoalveolar lavage saliva samples underwent metatranscriptome profiling examine associations microbiomes. Pairwise comparisons assessed differentially abundant bacteria species. Total bacterial load was similar between groups, no...
Chronic hyperglycemia and hypercholesterolemia have been shown to alter ionic currents in vascular smooth muscle. We tested the hypothesis that combined effect of hyperlipidemia (diabetic dyslipidemia) would increase Ca 2 + -sensitive K (K ) current as a compensatory response an intracellular concentration. also hypothesized exercise training prevent this elevation current. Miniature Yucatan swine were randomly assigned five groups: control, standard pig chow (C, n = 6); hyperlipidemic,...