The role of transforming growth factor-β1 (TGF-β1) in normal human fracture healing has been previously demonstrated. objective the present study was to examine biocompatibility TGF-β1-silk fibroin-chitosan (TGF-β1-SF-CS) three-dimensional (3D) scaffolds order construct an ideal scaffold for bone tissue engineering. We added TGF-β1 directly SF-CS a 3D first time, best our knowledge, and performed evaluations determine whether it may have potential applications as factor delivery device. Bone...

Abstract The current study aimed to investigate the mechanism by which miR‐454 influences progression of heart failure (HF) in relation neural precursor cell expressed, developmentally downregulated 4‐2 (NEDD4‐2)/tropomyosin receptor kinase A (TrkA)/cyclic adenosine 3',5'‐monophosphate (cAMP) axis. Sprague‐Dawley rats were used establish a HF animal model via ligation left anterior descending branch coronary artery. cardiomyocyte H9c2 cells treated with H 2 O stimulate oxidative stress...

Schwann cells (SCs) are the major constituting peripheral nerve structure and function, also secret a variety of neurotrophic factors. cell (SC) transplantation has recently emerged as promising therapeutic strategy for spinal cord injury (SCI). In present study, ability genetically modified SCs producing high levels glial line‑derived factor (GDNF) to promote repair was assessed. The GDNF gene transduced into SCs. engineered were characterized by their express secrete biologically active...

Scaffold fabrication and biocompatibility are crucial for successful bone tissue engineering. Nanometer hydroxyapatite (nHAP) combined with collagen (COL) is frequently utilized as a suitable osseous scaffold material. Furthermore, growth factors, including morphogenetic protein‑2 (BMP‑2), used to enhance the properties. The present study blending freeze‑drying methods develop BMP‑2‑nHAP‑COL scaffold. An ELISA was performed determine BMP‑2 release rate from Flow cytometry identify rat...

The combination treatment for mix infantile hemangiomas (IHs) using oral propranolol with topical timolol maleate was not well documented in the literature. aim of this study to evaluate therapeutic effects and safety along or alone treating mixed IHs maxillofacial regions.Between March 2013 June 2014, a total 31 patients regions were recruited randomly divided into experimental control groups. Fourteen group (A) treated proranolol maleate, 17 (B) underwent orally alone. maximal duration...

Objective. Infantile hemangiomas (IHs) are the most common benign tumors in infancy. The purpose of this study was to effects propranolol on function human umbilical vein endothelial cells (HUVECs), order preliminarily elucidate mechanism treatment IHs. Methods. HUVECs were treated with different concentrations (30 μM, 60 90 and 120 μM) or without VEGF. Their proliferation, migration, invasion, adhesion, tube formation ability tested by using CCK-8, wound healing assay, transwell, cell...

The objective of this study was to discuss the construction method, characterization, and biocompatibility three-dimensional silk fibroin-chitosan (SF-CS) scaffolds which met requirements bone tissue engineering scaffolds. Silk fibroin (SF) chitosan (CS) were mixed at different ratios —3 7, 5 5, 7 3— fabricate composite materials. To find out optimum mixing ratio SF CS, parameters such as pore size, porosity, water absorption, mechanical properties evaluated. Osteoblast cells hFOB1.19 seeded...

Background Many reconstructive methods for facial nerve defects have been described previously, such as the greater auricular graft, sural or hypoglossal-facial anastomosis. Herein, we want to instruct a new technique of repairing zygomatic marginal mandibular branches using upper buccal cervical when face segment with wide gaps between stumps. Methods The distal part peripheral tissue was removed repair branches. Clinical and electromyographic examinations were employed investigate clinical...

The objective of the study was to discuss biocompatibility vascular endothelial growth factor-silk fibroin-chitosan (VEGF-SF-CS) scaffolds. To offer an ideal scaffold for bone tissue engineering, author added factor (VEGF) into silk (SF-CS) directly reconstruct a three-dimensional first time, SF-CS loaded with VEGF and evaluated as factor-delivery device. Human fetal osteoblast cell seeded on VEGF-SF-CS scaffolds On scaffolds, adhesion rate increased time went on. Scanning electron...

Abstract Background Tumorigenesis is thought to be the consequence of gene mutation and disordered expression. However, detailed molecular mechanism underlying development progress colon cancer have not been elucidate completely. This study aimed find out genes associated with biological pathways involved in transformation tumorigenesis. Methods Normal intestinal cell line 6 (IEC-6) cells were transformed by treatment cancerogenic agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) Phorbol...

The aim of the present study was to examine biocompatibility transforming growth factor-β1-silk fibroin-chitosan (TGF-β1-SF-CS) scaffolds. In order provide an ideal scaffold for use in bone tissue engineering, TGF-β1 introduced into SF‑CS reconstruct a three dimensional scaffold, following which hFOB1.19 osteoblast cells were seeded onto TGF‑β1‑SF‑CS and On scaffolds, cell adhesion rate increased time‑dependent manner. Scanning electron microscopy revealed that grew actively exhibited normal...

The aim of the present study was to assess efficacy and safety topical timolol maleate combined with oral propranolol for parotid infantile hemangiomas. Between October 2012 April 2014, administered orally at a dose 1.0-1.5 mg/kg/day 22 infants proliferating hemangiomas in Department Oral Maxillofacial Surgery (Hospital Stomatology, China Medical University, Shenyang, Liaoning, China). A small amount 0.5% eye drop solution topically applied medical cotton swabs area lesion twice day, every...

The aim of our study was to assess the efficacy and safety oral propranolol for treatment parotid infantile hemangiomas. Between October 2009 January 2013, given orally 30 infants with proliferating hemangiomas at a dose 1.0 1.5 mg/kg per day in department. patients included 12 male 18 female infants, aged between 2 13 months, median 5.9 months. lesions were located region measured from cm × 0.5 6 8 3 volume. Oral administered once daily mean duration 22.7 weeks (range, 14-32 wk). Follow-up...