A5060450800- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Corrosion Behavior and Inhibition
- RNA and protein synthesis mechanisms
- Peroxisome Proliferator-Activated Receptors
- Genetic and phenotypic traits in livestock
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- Bacterial Genetics and Biotechnology
- Concrete Corrosion and Durability
- MicroRNA in disease regulation
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Optical Systems and Laser Technology
- Animal Genetics and Reproduction
- interferon and immune responses
- Hydrogen embrittlement and corrosion behaviors in metals
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Adipose Tissue and Metabolism
- Antimicrobial Peptides and Activities
Southwest Minzu University
2023-2025
Ministry of Agriculture and Rural Affairs
2023-2024
Zhejiang University
2023-2024
Zhengzhou University
2024
Southeast University
2021-2023
Northwest A&F University
2016-2022
Southeast University
2021
North China Electric Power University
2015
Long non-coding RNAs (lncRNAs) are a class of RNA molecules that longer than 200 nucleotides and were initially believed to lack encoding capability. However, recent research has found open reading frames (ORFs) within lncRNAs, suggesting they may have coding capacity. Despite this discovery, the mechanisms by which lncRNA-encoded products involved in cancer not well understood. The current study aims investigate whether lncRNA HCP5-encoded promote triple-negative breast (TNBC) regulating...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR) system has recently been developed into a powerful genome-editing technology, as it requires only two key components (Cas9 protein and sgRNA) to function further enables multiplex genome targeting homology-directed repair (HDR) based precise editing in wide variety of organisms. Here, we report novel interesting strategy by using the Drosha-mediated sgRNA-shRNA structure direct Cas9 for editing. For assay,...
RNA editing is the process of modifying molecules by inserting, deleting, or substituting nucleotides. In flowering plants, occurs predominantly in RNAs encoded organellar genomes mitochondria and chloroplasts, main type involves substitution cytidine with uridine at specific sites. Abnormal plants can affect gene expression, organelle function, plant growth, reproduction. this study, we report that ATPC1, gamma subunit ATP synthase Arabidopsis has an unexpected role regulation multiple...
As a transcription factor, Nuclear Receptor Subfamily 4 Group A Member 1 (NR4A1) binds to downstream target genes participate in cell proliferation and differentiation. We found that the NR4A1 reached highest expression at 60 h after differentiation of goat intramuscular preadipocytes. Overexpression increased number intracellular lipid droplets up-regulated adipocyte-differentiation-related marker including AP2, SREBP1, ACC, GPAM, DGAT2, while relative levels Pref-1 HSL were significantly...
Ferroptosis is a form of regulated cell death driven by the accumulation lipid hydroperoxides. Regulation ferroptosis might be beneficial to cancer treatment. Non-coding RNAs (ncRNAs) are class RNA transcripts that generally cannot encode proteins and have been demonstrated play critical roles in regulating ferroptosis. Herein, we developed ncFO, ncRNA-ferroptosis association database, document manually curated predicted ncRNAs associated with Collectively, ncFO contains 90 experimentally...
Base editing has emerged as a revolutionary technology for single nucleotide modifications. The cytosine and adenine base editors (CBEs ABEs) have demonstrated great potential in clinical fundamental research. However, screening isolating target-edited cells remains challenging. In the current study, we developed universal Adenine Cytosine Base-Editing Antibiotic Resistance Screening Reporter (ACBE-ARSR) improving efficiency. To develop reporter, CBE-ARSR was first constructed shown to be...
The Argonaute proteins are present in all three domains of life, which archaea, bacteria, and eukarya. Unlike the eukaryotic proteins, use small RNA guides to target mRNAs, some prokaryotic (pAgos) a DNA guide interfere with and/or targets. However, mechanisms pAgo natural function remain unknown. Here, we investigate mechanism by from Natronobacterium gregoryi (NgAgo) targets plasmid bacteriophage T7 using heterologous Escherichia coli-based model system. We show that NgAgo expressed...
The prokaryotic Argonaute proteins (pAgos) have been reported to cleave or interfere with DNA targets in a guide-dependent independent manner. It is often difficult characterize pAgos vivo due the extreme environments favored by their hosts. In present study, we expressed functional Thermus thermophilus pAgo (TtAgo) E. coli BL21 (DE3) cells at 37 °C. Initial attempts express TtAgo BL21(DE3) °C failed. This was not because of mediated general toxicity host cells, but instead TtAgo-induced...
RNA editing in chloroplast and mitochondrion transcripts of plants is an important type post-transcriptional modification which members the multiple organellar factor gene family (MORF) play a crucial role. However, systematic identification characterization MORF Brassica napus still lacking.
The aim of this study was to elucidate the effect
Abstract RPOTp is the nuclear‐encoded plastid‐targeted RNA polymerase and plays a crucial role in chloroplast gene expression. Transcripts plant organelles are altered by conversion of cytidine (C) to uridine (U) at specific positions through editing. However, whether involved editing remains unclear. Here, C‐to‐U multiple sites Arabidopsis chloroplasts uncovered. Multiple organellar factor 2 (MORF2) required for most chloroplasts. identified from co‐immunoprecipitation targets MORF2. The...
Goat intramuscular fat (IMF) deposition is precisely regulated by many key genes as well transcription factors. Nevertheless, the potential of regulators goat IMF remains undefined. In this work, we reported that factor FOS expressed at a low level early differentiation stage and high in late differentiation. The overexpression inhibited adipocyte lipid accumulation significantly downregulated expressions PPARγ, C/EBPβ, C/EBPα, AP2, SREBP1, FASN, ACC, HSL, ATGL. Consistently, knockdown FOS,...
This paper the reduction of Chang'e no.3 soft landing and obstacle avoidance phase are studied.And based on that, there is an orbital control scheme with aim consumption least fuel.In this scheme, dynamic programming model that aims at consuming fuel set up for main stage.Combined genetic algorithm, we get optimal scheme.During phase, use texture analysis to quickly identify surface imaging automatically look a safe area in order avoid obstacle.
Abstract Background Long non-coding RNAs (lncRNAs) is widely described as a class of RNA longer than 200 nucleotides without encoding capability. But recent years, more and open reading frames (ORFs) have been found in lncRNAs which indicate they coding capacity. the mechanisms products cancer are mostly unknown. We previously shown lncRNA HCP5 an oncogene triple negative breast (TNBC), aim current study was to investigate if protein promotes TNBC by regulating ferroptosis. Methods use...