A5001007137- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Animal Nutrition and Physiology
- High Altitude and Hypoxia
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Polymer composites and self-healing
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Growth Hormone and Insulin-like Growth Factors
- Cancer, Hypoxia, and Metabolism
- Adsorption and biosorption for pollutant removal
- Bone and Joint Diseases
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Esophageal Cancer Research and Treatment
- Advanced Sensor and Energy Harvesting Materials
- Bioinformatics and Genomic Networks
- Food Waste Reduction and Sustainability
- Control Systems in Engineering
- Drug Transport and Resistance Mechanisms
- Genomic variations and chromosomal abnormalities
- Hedgehog Signaling Pathway Studies
- Municipal Solid Waste Management
Northwest A&F University
2014-2025
Third Affiliated Hospital of Sun Yat-sen University
2024-2025
Sun Yat-sen University
2024-2025
Zhejiang Cancer Hospital
2025
Shaoxing University
2025
Northwestern Polytechnical University
2022-2024
Dalian University of Technology
2024
Zhejiang Ocean University
2023
Zhejiang University
2023
Changzhi Medical College Peace Hospital
2018-2019
The hypoxic environment imposes severe selective pressure on species living at high altitude. To understand the genetic bases of adaptation to altitude in dogs, we performed whole-genome sequencing 60 dogs including five breeds continuous altitudes along Tibetan Plateau from 800 5100 m as well one European breed. More than 150× coverage for each breed provides us with a comprehensive assessment polymorphisms Mastiffs. Comparison different reveals strong signals population differentiation...
Abstract Artificial materials with biomimic self‐healing ability are fascinating, however, the balance between mechanical properties and performance is always a challenge. Here, robust, highly stretchable elastomer dynamic reversible multi‐networks based on polyurethane matrix cyclodextrin‐assembled nanosheets proposed. The introduction of cyclodextrin nano‐assemblies abundant surface hydroxyl groups not only forms multiple interfacial hydrogen bonding but also enables strain‐induced...
No appropriate studies have been conducted that compare the biomechanical properties of different fenestration positions in deep circumflex iliac artery (DCIA)-vascularized bone grafts for femoral head necrosis (ONFH) treatment. In this study, we aimed to explore locations DCIA-vascularized grafting ONFH treatment using FEA and clinical retrospective analysis. We simulated an flap transplantation with a vascularized tip by finite element analysis (FEA). Patients were divided into four groups...
BACKGROUND Esophageal squamous cell carcinoma (ESCC) is a common malignancy in China, often diagnosed at an advanced stage, with poor prognosis. Standard treatments such as definitive chemoradiotherapy offer limited survival benefits. Recent advances immune checkpoint inhibitors combined chemotherapy have shown promise, but their effectiveness and safety conjunction radiotherapy for unresectable ESCC require further exploration. AIM To assess the of induction chemoimmunotherapy followed by...
Tibetan chicken (TBC) is one of the native poultry species that well adapted to high-altitude environment Qinghai-Tibet Plateau. To elucidate genetic mechanisms underlying adaptation, transcriptomes five tissues (heart (HE), lung (LU), liver (LI), ovary (OV), and abdominal fat (AB)) were compared between TBCs Roman chickens (RMCs) inhabiting plateau for year. Moreover, weighted gene co-expression network analysis (WGCNA) was applied detect tissue-associated modules hub genes. A total 1105,...
Abstract The clustered regularly interspaced short palindromic repeats (CRISPR) system has recently been developed into a powerful genome-editing technology, as it requires only two key components (Cas9 protein and sgRNA) to function further enables multiplex genome targeting homology-directed repair (HDR) based precise editing in wide variety of organisms. Here, we report novel interesting strategy by using the Drosha-mediated sgRNA-shRNA structure direct Cas9 for editing. For assay,...
In recent years, genome engineering technology has provided unprecedented opportunities for site-specific modification of biological genomes. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 is one such means that can target a specific locus. It been applied in human cells and many other organisms. Meanwhile, to efficiently enrich targeted cells, several surrogate systems have also developed. However, very limited information exists on the...
Tibetan pigs have survived at high altitude for millennia and they a suite of adaptive features to tolerate the hypoxic environment. However, molecular mechanisms underlying regulation hypoxia-adaptive phenotypes not been completely elucidated. In this study, we analyzed differentially expressed genes (DEGs), biological pathways constructed co-expression networks using whole-transcriptome microarrays from lung tissues Duroc both low altitude. A total 3,066 DEGs were identified list was...
Egg production of hens is related to ovarian follicles development. The hierarchical follicle development accompanies the deposition a large amount yolk precursor. aim this study was illustrate effects strain and age on egg production. experiment compared synthesis, transport, in 3 groups hens: one high-yield commercial hybrid laying breed (Jinghong No.1) 2 stages (35 wk 75 wk; JH35, JH75) Chinese native (Lueyang Black-Boned chicken) at 35 (LY35). results showed that number JH35 JH75...
Purpose: To investigate the effect and mechanism of nebivolol on aortic remodeling in N-nitro-l-arginine methyl ester (l-NAME)-induced hypertension. Methods: Male Sprague–Dawley rats were treated with equal volumes drinking water or l-NAME (60 mg/kg/day), alone combination (8 mg/kg/day) atenolol (80 by gavage for 8 weeks. Systolic blood pressure (SBP), morphometry, plasma nitric oxide (NO) levels, synthase (NOS) activity, relaxation aorta to acetylcholine determined. Protein expression...
Overfeeding reduces laying performance in broiler breeder hens, which is associated with obesity, hepatic steatosis and systemic inflammation. To unravel the underlying mechanisms governing effect of feeding regimes on energy metabolism egg production, a transcriptomics approach was carried out for screening differentially expressed genes (DEGs) ovary, liver adipose tissues chickens under ad libitum restricted feeding.It showed that 289, 388 204 DEGs were identified adipose, respectively....
One-dimensional nanomaterials have become one of the most available nanoreinforcing agents for developing next-generation high-performance functional self-healing composites owing to their unique structural characteristics and surface electron structure. However, nanoscale control, regulation, crystal growth are still enormous challenges in synthesis specific one-dimensional nanomaterials. Here, oxygen-defective MoO
The clustered regularly interspaced short palindromic repeat ( CRISPR )/CRISPR‐associated protein 9 (Cas9) system has recently emerged as a simple, yet powerful genome engineering tool, which been widely used for modification in various organisms and cell types. However, screening biallelic genome‐modified cells is often time‐consuming technically challenging. In this study, we incorporated two different surrogate reporter cassettes into paired donor plasmids, were both the reporters...
Yeast two-hybrid (Y2H) methods are powerful tools for detecting protein–protein interactions. The traditional Y2H method has been widely applied to screen novel protein interactions since it was established two decades ago. high false-positive rate of the drove development modified systems. Here, we describe a system using zinc-finger nucleases (ZFNs). ZFNs contain functional domains, DNA-binding domain (ZFP) and non-specific nuclease (FokI). In this system, bait is expressed as fusion with...