A5077573090- Peroxisome Proliferator-Activated Receptors
- Renin-Angiotensin System Studies
- Eicosanoids and Hypertension Pharmacology
- Nitric Oxide and Endothelin Effects
- Adipose Tissue and Metabolism
- Hormonal Regulation and Hypertension
- Cardiovascular Function and Risk Factors
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Heavy Metal Exposure and Toxicity
- Genetic Neurodegenerative Diseases
- Microfluidic and Capillary Electrophoresis Applications
- Cardiac Ischemia and Reperfusion
- Neurological Disease Mechanisms and Treatments
- Barrier Structure and Function Studies
- Air Quality and Health Impacts
- Cholesterol and Lipid Metabolism
- Mercury impact and mitigation studies
- Neuroscience and Neuropharmacology Research
- Neuropeptides and Animal Physiology
- Electrochemical sensors and biosensors
- Fatty Acid Research and Health
- Sulfur Compounds in Biology
- Advanced Glycation End Products research
- Signaling Pathways in Disease
Instituto Nacional de Cardiología
2015-2024
Instituto Nacional de Cardiologia
2012
Instituto Politécnico Nacional
1998-2000
Center for Research and Advanced Studies of the National Polytechnic Institute
1998
We studied hydroxyeicosatetraenoic acid (HETE) release in response to ANG II from preglomerular microvessels (PGMVs), the vascular segment governing changes renal resistance. PGMVs were isolated Sprague-Dawley rats and incubated with NADPH hormones at 37°C. Eicosanoids extracted, cytochrome P-450 (CYP)-derived HETEs purified quantitated by negative chemical ionization gas chromatography-mass spectroscopy. produced primarily 20- 19-HETEs, namely, 7.9 ± 1.7 2.2 0.5 ng/mg protein, respectively....
Peroxisome proliferator-activated receptors (PPAR) play a critical physiological role in energy homeostasis, inflammation, and protective cardiovascular function. We assessed the antioxidant effect of clofibrate-induced receptor alpha (PPARα) stimulation on ischemic myocardium myocardial morphology hemodynamics. Male Wistar rats (300 g) were distributed into following groups: (1) Sham, (2) ischemia vehicle treated (MI-V), (3) clofibrate [100 mg/kg/ intraperitoneally) (MI-C). Reactive oxygen...
N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) is a potent natural inhibitor of hematopoietic stem cell proliferation which degraded mainly by angiotensin-converting enzyme (ACE). In vitro, Ac-SDKP inhibits collagen production cardiac fibroblasts; while in vivo it blocks deposition the left ventricle (LV) rats with hypertension or myocardial infarction (MI). addition, reportedly prevents and reverses macrophage infiltration LV MI. We tested hypothesis that when infused at doses cause plasma...
We investigated whether fenofibrate, metformin, and their combination generate cardioprotection in a rat model of type 2 diabetes (T2D) acute myocardial infarction (AMI). Streptozotocin-induced diabetic- (DB-) rats received 14 days either vehicle, or immediately after underwent ischemia/reperfusion (I/R). Fenofibrate plus metformin generated DBI/R model, reported as decreased coronary vascular resistance, compared to DBI/R-Vehicle, smaller infarct size, increased cardiac work. The subchronic...
Renin-angiotensin system (RAS) activation promotes oxidative stress which increases the risk of cardiac dysfunction in metabolic syndrome (MetS) and favors local insulin resistance. Fibrates regulate RAS improving MetS, type-2 diabetes cardiovascular diseases. We studied effect fenofibrate treatment on myocardic signaling pathway Angiotensin II (Ang II)/Angiotensin type 1 receptor (AT1) its relationship with myocardial resistance MetS rats under heart ischemia. Control were assigned to...
Numerous studies have supported a role for oxidative stress in the development of ischemic damage and endothelial dysfunction. Crataegus oxyacantha (Co) Rosmarinus officinalis (Ro) extracts are polyphenolic-rich compounds that proven to be efficient treatment cardiovascular diseases. We studied effect from Co Ro on myocardial associated with status production different vasoactive agents. Rats were assigned following groups: (a) sham; (b) vehicle-treated infarction (MI) (MI-V); (c)...
Rosiglitazone (RGZ), a peroxisome proliferator-activated receptor gamma (PPARγ) ligand, has been reported to act as insulin sensitizer and exert cardiovascular actions. In this work, we hypothesized that RGZ exerts PPARγ-dependent regulation of blood pressure through modulation angiotensin-converting enzyme (ACE)-type 2 (ACE2)/angiotensin-(1-7)/angiotensin II type-2 (AT2R) axis in an experimental model high pressure. We carried on experiments normotensive (Sham) aortic coarctation...
This study attempts to elicit whether the level of hyperglycemia in an early stage diabetic nephropathy changes renal expression claudins-2 and -5 determine involvement glucose-induced oxidative stress. Streptozotocin-induced type-1 type-2 (DM1, DM2)-rat models were used. At 14-week old, rats placed metabolic cages evaluate proteinuria, creatinine clearance, electrolyte excretion. Proximal tubules glomeruli isolated analyzed by Western blot immunofluorescence. Renal stress metalloproteinase...
Essential fatty acids have an important effect on oxidative stress-related diseases. The Huntington's disease (HD) is a hereditary neurologic disorder in which stress caused by free radicals damage mechanism. HD experimental model induced quinolinic acid (QUIN) has been widely used to evaluate therapeutic effects of antioxidant compounds. aim this study was test whether the content olive- or fish-oil-rich diet prevents against QUIN-related rats. Rats were fed during 20 days with (15% w/w)....
Objective To evaluate the contribution of nitric oxide to regulation angiotensin II-induced renal vasoconstriction in normotensive rats and with aortic coarctation-induced hypertension. Methods We evaluated vascular reactivity nonischemic kidney II without synthesis inhibitor (NG-nitro-L-arginine methyl ester) isolated perfused kidney. The nitrite concentration perfusate was measured as an index released activity synthase tissue determined by production [3H]-L-citrulline. Results perfusion...
Low levels of chronic lead exposure can produce hypertension and endothelial dysfunction, which could be associated with oxidative stress, changes in vascular tone an imbalance endothelial-derived vasoconstriction vasodilator factors. The aim was to investigate the effect lead-exposure on angiotensin II-induced isolated perfused kidney microvessels. Male Wistar rats (230—250 g) were treated for 12 weeks acetate (100 ppm, Pbgroup) or pure water (control group). We evaluated reactivity kidneys...
Myocardial infarction (MI) has been associated with an inflammatory response and a rise in TNF-α, interleukin (IL)-1β, IL-6. Peroxisome proliferator-activated receptors (PPARs) promote decreased expression of molecules. We aimed to study whether PPAR stimulation by clofibrate decreases inflammation reduces infarct size rats MI. Male Wistar were randomized into 3 groups: control, MI + vehicle, (100 mg/kg). Treatment was administered for consecutive days, previous 2 h induced increase protein...
We investigated the involvement of cyclooxygenase-2 (COX-2) and renin-angiotensin system in N(G)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension. Male Wistar rats were treated with L-NAME (75.0 mg·(kg body mass)(-1)·day(-1), their drinking water) for different durations (1-33 days). COX-2 renin mRNA measured using real-time PCR renal cortex, prostanoids assessed perfusate, whereas angiotensin II (Ang II) Ang (1-7) quantified plasma. In some rats, nitric oxide synthase inhibition...
Myocardial infarction (MI) initiates an inflammatory response that promotes both beneficial and deleterious effects. The early helps the myocardium to remove damaged tissue; however, a prolonged later brings cardiac remodeling characterized by functional, metabolic, structural pathological changes. Current pharmacological treatments have failed reverse ischemic-induced damage. Therefore, our aim was study if clofibrate treatment capable of decreasing inflammation apoptosis, ventricular...
Lesions caused by high glucose (HG), hypoxia/reperfusion (H/R), and the coexistence of both conditions in cardiomyocytes are linked to an overproduction reactive oxygen species (ROS), causing irreversible damage macromolecules cardiomyocyte as well its ultrastructure. Fenofibrate, a peroxisome proliferator-activated receptor alpha (PPARα) agonist, promotes beneficial activities counteracting cardiac injury. Therefore, objective this work was determine potential protective effect fenofibrate...
Chronic hyperglycemia results in morphological and functional alterations of the kidney microvascular damage, leading to diabetic nephropathy (DN). Since DN progresses irreversible renal it is important elucidate a pharmacological strategy aimed for treating early stage. Here, we used type 2 rat model induce show nephroprotective effect following stimulation PPAR-α, which stabilized tight junction components claudin-2, claudin-5, claudin-16. At 14 weeks old, streptozotocin-induced DN,...