A5090711377- Bacterial biofilms and quorum sensing
- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Plant Pathogenic Bacteria Studies
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Evolution and Genetic Dynamics
- Antibiotic Resistance in Bacteria
- Evolutionary Game Theory and Cooperation
- Antimicrobial Peptides and Activities
- Gut microbiota and health
- Probiotics and Fermented Foods
- Plant Pathogens and Fungal Diseases
- Protist diversity and phylogeny
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Natural Products and Biosynthesis
- Oral microbiology and periodontitis research
- CRISPR and Genetic Engineering
- biodegradable polymer synthesis and properties
- Biopolymer Synthesis and Applications
- Enzyme function and inhibition
- Antimicrobial agents and applications
- Biochemical and Structural Characterization
University of Massachusetts Dartmouth
2012-2024
University of Canterbury
2000-2017
Tufts University
2002-2014
Boston University
2002
Zero to Three
2001
Pseudomonas fluorescens are common soil bacteria that can improve plant health through nutrient cycling, pathogen antagonism and induction of defenses. The genome sequences strains SBW25 Pf0-1 were determined compared to each other with P. Pf-5. A functional genomic in vivo expression technology (IVET) screen provided insight into genes used by its natural environment an improved understanding the ecological significance diversity within this species.
Complications arising from antibiotic-resistant bacteria are becoming one of the key issues in modern medicine. Members drug-resistant Enterobacteriaceae spp. include opportunistic pathogens (e.g., Salmonella spp.) that among leading causes morbidity and mortality worldwide. Overgrowth these is considered a hallmark intestinal dysbiosis. Microcins (small antimicrobial peptides) produced by some gut commensals can potentially cure conditions inhibiting have been proposed as viable alternative...
The effectiveness of antibiotic molecules in treating Pseudomonas aeruginosa pneumonia is reduced as a result the dissemination bacterial resistance. existence communication systems, such quorum sensing, has provided new opportunities treatment. Lactonases efficiently quench acyl-homoserine lactone-based implicating these enzymes potential anti-Pseudomonas drugs that might be evaluated pneumonia.The aim present study was to evaluate ability lactonase called SsoPox-I reduce mortality rat P....
Losing and then regaining flagella The ability to adapt changes in the function of gene regulators, as opposed structural genes, is a crucial aspect evolutionary change. Taylor et al. mutated central regulator for formation bacterium Pseudomonas fluorescens . They put flagella-free bacteria under strong selection pressure regain mobility. regained lost flagella, motility, within 4 days. Two stereotypical mutations diverted an evolutionarily related that normally controls nitrogen uptake...
Herbicides are frequently released into both rural and urban environments. Commercial herbicide formulations induce adaptive changes in the way bacteria respond to antibiotics. Salmonella enterica sv. Typhimurium Escherichia coli were exposed common co-formulants of formulations, S. was active ingredients dicamba, 2,4-D glyphosate determine what commercial caused this effect. Co-formulants Tween80 carboxymethyl cellulose induced response, but pattern responses differed from ingredients,...
The gastrointestinal (GI) tract is the reservoir for multidrug resistant (MDR) pathogens, specifically carbapenem-resistant (CR) Klebsiella pneumoniae and other Enterobacteriaceae, which often lead to spread of antimicrobial resistance genes, severe extraintestinal infections, lethal outcomes. Selective GI decolonization has been proposed as a new strategy preventing transmission body sites minimizing spreading susceptible individuals. Here, we purify to-date uncharacterized class IIb...
We report the isolation of insertional mutations to pstC and pstA genes phosphate‐specific transport ( pst ) operon that results in loss biofilm formation by Pseudomonas aureofaciens PA147‐2. Consistent with known roles Pst system Escherichia coli aeruginosa , both P. mutants were demonstrated have defects inorganic phosphate (P i repression Pho regulon expression. Subsequently, wild type was shown require a threshold concentration extracellular P . The two‐component regulatory pair...
Studies were undertaken to determine the genetic needs for survival of Pseudomonas fluorescens Pf0-1, a gram-negative soil bacterium potentially important biocontrol and bioremediation, in soil. In vivo expression technology (IVET) identified 22 genes with elevated relative laboratory media. Soil-induced sequences included probable functions nutrient acquisition use, gene regulation. Ten sequences, lacking similarity known genes, overlapped divergent revealing novel organization at those...
Genome mining of the Gram-negative bacterium Pseudomonas fluorescens Pf0-1 showed that strain possesses a silent NRPS-based biosynthetic gene cluster encoding new lipopeptide; its activation required repair global regulator system. In this paper, we describe genomics-driven discovery and characterization associated secondary metabolite gacamide A, lipodepsipeptide forms family lipopeptides. The compound has moderate, narrow-spectrum antibiotic activity facilitates bacterial surface motility.
Background Many soil-inhabiting bacteria are known to produce secondary metabolites that can suppress microorganisms competing for the same resources. The production of antimicrobial compounds is expected incur fitness costs producing bacteria. Such form basis models on co-existence antibiotic-producing and non-antibiotic strains. However, so far studies quantifying antibiotic by scarce. current study reports possible costs, Pseudomonas fluorescens Pf0-1, a soil bacterium induced...
The wealth of studies on microbial communities has revealed the complexity and dynamics composition in many ecological settings. Fewer probe functional interactions community members. Function as a whole may not be fully by characterizing individuals. In our two-species model community, we find an emergent trait resulting from interaction soil bacteria Pseudomonas fluorescens Pf0-1 Pedobacter sp. V48. Observation traits suggests there functions that are predicted based priori knowledge These...
ABSTRACT AdnA is a transcription factor in Pseudomonas fluorescens that affects flagellar synthesis, biofilm formation, and sand adhesion. To identify the regulon, we used promoterless Tn 5 - lacZ element to study phenotypes of insertion mutants presence absence AdnA. Of 12,000 insertions, identified seven different putative open reading frames (ORFs) activated by (named aba for AdnA). aba120 aba177 showed homology flgC flgI , components basal body flagella aeruginosa . Two other aba18 aba51...
Pseudomonas species can exhibit phenotypic variation resulting from gacS or gacA mutation. P. fluorescens Pf0-1 is a mutant and exhibits pleiotropic changes following the introduction of functional allele. GacA enhances biofilm development while reducing dissemination in soil, suggesting that alternative Gac phenotypes enable sp. to exploit varied environments.
The annotated genome sequences of prokaryotes seldom include overlapping genes encoded opposite each other by the same stretch DNA. However, antisense transcription is becoming recognized as a widespread phenomenon in eukaryotes, and examples have been linked to important biological processes. Pseudomonas fluorescens inhabits aquatic terrestrial environments, can be regarded an environmental generalist. genetic basis for this ecological success not well understood. In previous search...
Genome sequences are annotated by computational prediction of coding sequences, followed similarity searches such as BLAST, which provide a layer possible functional information. While the existence processes alternative splicing complicates matters for eukaryote genomes, view bacterial genomes linear series closely spaced genes leads to assumption that annotations predict arrangements completely describe capacity genomes. We undertook proteomic study identify proteins expressed Pseudomonas...
Knowledge of the genetic basis for bacterial survival and persistence in soil is a critical component development successful biological control strategies understanding ecological success bacteria. We found locus specifying polyphosphate kinase (ppk) nonpredicted antisense RNA (iiv8) Pseudomonas fluorescens Pf0-1 to be necessary optimal competitive fitness LB broth culture sterile loam soil. lacking ppk iiv8 was more than 10-fold less against wild-type low inorganic phosphate. Studies...
AdnA in Pseudomonas fluorescens, an ortholog of FleQ P. aeruginosa, regulates both motility and flagellum-mediated attachment to various surfaces. A whole-genome microarray determined the transcriptome by comparing gene expression pattern wild-type Pf0-1 that Pf0-2x (adnA deletion mutant) broth culture. In absence AdnA, 92 genes was decreased, while 11 showed increased expression. Analysis 16 these fused lacZ confirmed results. Several were further evaluated for their role biofilm formation....
Abstract Background Pseudomonas fluorescens is a common inhabitant of soil and the rhizosphere environment. In addition to potential applications in biocontrol bioremediation, P. interest as model for studying bacterial survival fitness soil. A previous study using vivo expression technology (IVET) identified 22 genes Pf0-1 which are up-regulated during growth Massachusetts loam soil, subset important Despite this other information on adaptation downstream such or bioremediation diverse...
Pseudomonas fluorescens strains are important candidates for use as biological control agents to reduce fungal diseases on crop plants. To understand the ecological success of these bacteria and successful stable control, determination how colonize persist in soil environments is critical. Here we show that P. Pf0-1 negatively impacted by reduced water availability soil, but adapts persists. A pilot transcriptomic study colonizing moist dehydrated was used identify candidate genetic loci,...
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