A5101942455- Ginseng Biological Effects and Applications
- Autophagy in Disease and Therapy
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- MicroRNA in disease regulation
- Lipid metabolism and disorders
- Alcohol Consumption and Health Effects
- Natural product bioactivities and synthesis
- Curcumin's Biomedical Applications
- Phagocytosis and Immune Regulation
- Peroxisome Proliferator-Activated Receptors
- Biochemical effects in animals
- Computational Drug Discovery Methods
- Axon Guidance and Neuronal Signaling
- PARP inhibition in cancer therapy
- Epigenetics and DNA Methylation
- Advanced Glycation End Products research
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Machine Learning in Bioinformatics
- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
- Cell death mechanisms and regulation
- Retinoids in leukemia and cellular processes
South China Normal University
2024
Hubei University of Education
2024
Xiangya Hospital Central South University
2014-2023
Central South University
2014-2023
Hunan Provincial Center for Disease Control and Prevention
2023
Hunan Mawangdui Hospital
2023
Affiliated Hospital of Youjiang Medical University for Nationalities
2020-2023
Youjiang Medical College for Nationalities
2018-2020
University of British Columbia
2019
British Columbia Children's Hospital
2019
Abstract Umbilical cord‐mesenchymal stem cells (UC‐MSCs)‐derived exosomes have been considered as an effective treatment for ischemic stroke. CircRNA BBS2 (circBBS2) was demonstrated to be down‐regulated in patients with However, the role of UC‐MSCs‐derived exosomal circBBS2 stroke and potential mechanisms remain unclear. Hypoxia/reperfusion (H/R)‐exposed SH‐SY5Y middle cerebral artery occlusion (MCAO)‐treated rats were served vitro vivo models Target gene expression detected by qRT–PCR....
Abstract Energy restriction (ER) protects against cerebral ischemic injury, but the underlying mechanism remains largely unclear. Here, rats were fed ad libitum (AL) or on an alternate-day food deprivation intermittent fasting (IF) diet for 3 months, followed by middle artery occlusion (MCAO) surgery. The body weight, infarct volume, and neurological deficit score accessed at designated time points. ELISA, qRT-PCR, Western blotting used to determine cytokine secretion expression of SIRT6,...
ABSTRACT The fluorescence resonance energy transfer (FRET) two‐hybrid assay enables live‐cell detection of biomolecular complexes but faces high‐throughput screening (HTS) limitations due to laborious image analysis. We developed an automated platform using the Luminance‐Uniformity‐based Region Interest Selection (LURS) algorithm, accelerating processing 12‐fold (6 h 30 min) for three‐channel FRET imaging. Validation with standards (C32V: , ; CVC: ) matched reference values. Applied...
The turmeric derivative curcumin protects against cerebral ischemic injury. We previously demonstrated that activates peroxisome proliferator‐activated receptor‐γ (PPARγ), a ligand‐activated transcription factor involved in both neuroprotective and anti‐inflammatory signaling pathways. This study tested whether the effects of oxygen–glucose deprivation/reoxygenation (OGD/R)‐induced injury rat cortical neurons are mediated (at least part) by PPARγ. Curcumin (10 μM) potently enhanced PPARγ...
Panax notoginseng saponins (PNS) have been widely used in China to treat stroke. Accumulating evidence has found that microRNA (miR)-155 plays critical roles the pathology of ischemic Here we investigated whether PNS a protective effect against oxygen-glucose deprivation/reoxygenation (OGD/R)-induced focal inflammation and injury SH-SY5Y cells by regulating miR-155 expression. Treatment with at concentration less than 160 µg/mL had no on proliferation cell. In OGD/R-induced cells, treatment...
Regulatory T (Treg) cell-specific deletion of a gene interest is procedure widely used to study mechanisms controlling Treg development, homeostasis and function. Accordingly, several transgenic mouse lines have been generated that bear the Cre recombinase under control Foxp3 promoter either as random transgene insertion or knocked into endogenous locus, with Foxp3YFP-Cre strain mice being one most used. In an attempt generate cells lacked expression insulin receptor (Insr), we crossed...
Ephrin-B2 has been shown to participate in angiogenesis, but the underlying mechanisms involved remain unclear. In this study, a rat model of focal cerebral ischemia was prepared by middle artery occlusion, followed 24-hour reperfusion. Then, ephrin-B2 protein administered intracerebroventricularly for 3 consecutive days via micro-osmotic pump. Western blot assay and quantitative real-time reverse transcription PCR demonstrated expression levels angiopoietin-1 (Ang-1) mRNA penumbra cortex...
Background: Lysine-specific histone demethylase 1 (LSD1) is a well-known anti-cancer target for drug discovery. Novel reversible inhibitors of LSD1 are desirable to be developed. Objective: This study aimed build reliable predictive models evaluate the antineoplastic efficacy compounds and reveal structural foundation underlying inhibitory activity LSD1. Methods: Multiple artificial intelligence algorithms were utilized in development quantitative structure-activity relationship (QSAR)...
Stroke is a cerebrovascular disease that results in decreased blood flow. Although Panax notoginseng (PN), Chinese herbal medicine, has been proven to promote stroke recovery, its molecular mechanism remains unclear. In this study, middle cerebral artery occlusion (MCAO) was induced rats with thrombi generated by thread and subsequently treated PN. After that, staining 2,3,5-triphenyltetrazolium chloride employed evaluate the infarcted area, electron microscopy used assess ultrastructural...
Treatment with Panax notoginseng saponin (PNS) can prevent neurological damage in middle cerebral artery occlusion model rats to promote recovery after a stroke. However, the exact molecular mechanisms are unknown and require further study. In present study, mRNA sequencing was employed investigate differential gene expression between sham groups, PNS‑treated groups. Enrichment of data performed using Gene Ontology analysis Kyoto Encyclopedia Genes Genomes database. Hub genes were identified...