A5074227084- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Bone Metabolism and Diseases
- Cancer, Hypoxia, and Metabolism
- Sarcoma Diagnosis and Treatment
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Heat shock proteins research
- Pancreatic function and diabetes
- Bone health and osteoporosis research
- MicroRNA in disease regulation
- Neurological Disease Mechanisms and Treatments
- Stress Responses and Cortisol
- TGF-β signaling in diseases
- Circular RNAs in diseases
- Lung Cancer Treatments and Mutations
- Psoriasis: Treatment and Pathogenesis
- Autophagy in Disease and Therapy
- Cardiac electrophysiology and arrhythmias
- Endoplasmic Reticulum Stress and Disease
- Traditional Chinese Medicine Analysis
- Apelin-related biomedical research
- Microfluidic and Capillary Electrophoresis Applications
- Ion channel regulation and function
- Growth Hormone and Insulin-like Growth Factors
Lanzhou University
2011-2024
First Affiliated Hospital of Kunming Medical University
2024
Kunming Medical University
2024
First Hospital of Lanzhou University
2011-2024
The First Hospital of Kunming
2024
Wenzhou Medical University
2014-2023
First Affiliated Hospital of Wenzhou Medical University
2014-2023
Fudan University
2021-2023
Huazhong University of Science and Technology
2023
Jiangxi Science and Technology Normal University
2023
Reduced bone mineral density (BMD) is associated with an altered microbiota in senile osteoporosis.However, the relationship among gut microbiota, BMD and metabolic indexes remains unknown postmenopausal osteoporosis.In this study, fecal profiles for 106 individuals osteopenia (n=33) or osteoporosis (n=42) normal (n=31) were determined.An integrated 16S rRNA gene sequencing LC-MS-based metabolomics approach was applied to explore association of estrogen-reduced phenotype.Adjustments made...
A digital electrode array, on which drops of solvent can be electronically manipulated, allows automated steroid extraction from tiny tissue samples for diagnosis or research.
Background Long noncoding RNAs (lncRNAs) have been shown to be involved in the development and progression of lung cancer. However, roles lncRNAs cancer are not well understood. Methodology/Principal Findings We used a high-throughput microarray compare lncRNA messenger RNA (mRNA) expression profiles adenocarcinoma normal tissue (NT) samples. Several candidate adenocarcinoma-associated were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis....
Abstract Recent advances in genome engineering have further widened the gap between our ability to implement essentially any genetic change and understanding impact of these changes on cellular function. We lack efficient methods diagnose limiting steps engineered pathways. Here, we develop a generally applicable approach reveal within synthetic pathway. It is based monitoring metabolite dynamics simplified kinetic modelling differentiate putative causes product synthesis during start-up...
To understand the effects of low-frequency pulsed electromagnetic fields (PEMFs) on chronic bony pain, bone mineral density (BMD), strength and biochemical markers metabolism in patients osteoporosis.Using key words "pulsed fields" "osteoporosis", we searched PubMed for related studies published English from January 1996 to December 2007. We also China National Knowledge Infrastructure (CNKI) Chinese 2007.(1) all articles which referred magnetic osteoporosis either primary or secondary...
Abstract Context.—c-kit, a proto-oncogene, encodes the transmembrane tyrosine kinase receptor CD117 and is detected by flow cytometry in majority of cases acute myeloid leukemia. The prognostic significance presence c-Kit leukemia debated. Recently, c-kit inhibitors have been studied as possible therapies against hematopoietic malignancies; therefore, detection may important implications for treatment. Objectives.—In this study, we investigated expression granulocytic sarcoma (GS) using...
Previous evidence has indicated that the microRNA-125b (miR-125b) family plays important roles in regulation of cancer cell growth, development, differentiation, and apoptosis. However, whether they contribute to process adipocyte differentiation remains unclear. In present study, we revealed expression level miR-125b-5p, a member miR-125b family, was dramatically up-regulated during 3T3-L1 preadipocyte into mature adipocyte. Supplement miR-125b-5p cells promoted adipogenic as evidenced by...
Abstract Single-cell electrical phenotyping (e.g., specific membrane capacitance (C m ) and cytoplasm conductivity (σ p )) has long been regarded as potential label-free biophysical markers in tumor status evaluation. However, previous studies only reported the differentiation of cell lines without classifying real samples using cellular properties. In this study, two types mouse models were constructed by injecting (A549 H1299), respectively. Then portions retrieved for immunohistochemistry...
Therapeutics used to treat central nervous system (CNS) injury are designed promote axonal regeneration and inhibit cell death. Previous studies have shown that liraglutide exerts potent neuroprotective effects after brain injury. However, little is known if treatment has spinal cord (SCI). This study explores the of associated underlying mechanisms. Our results showed could improve recovery by decreasing apoptosis as well increasing microtubulin acetylation, autophagy. Autophagy inhibition...
Zinc finger MIZ-type containing 1 (ZMIZ1) is a transcriptional coactivator related to the protein inhibitors of activated STATs (PIAS) family. Mounting evidence suggests that ZMIZ1 plays crucial role in occurrence and development cancers. The function tongue squamous cell carcinoma (TSCC) mechanisms underpinning its this disease have not been fully clarified. We performed qualitative expression analyses using immunohistochemistry 20 patient-derived, paraffin-embedded TSCC tissue sections....
ABSTRACT Previously, a significantly upregulated lncRNA, LINC01512, in lung adenocarcinoma (LAD) was obtained, while its biological function and molecular mechanisms were unclear. The expression level of LINC01512 estimated by qPCR from 100 pairs LAD NT samples. correlation to clinical data patients analyzed. knocked down overexpressed SPCA‐1 A549 cell lines lentivirus‐mediated technology, the oncological behavioral changes cells observed, as well as, tumorigenicity experimental nude mice....
This study was designed to determine the effects of pulsed electromagnetic fields (PEMF) on mRNA expression receptor activator NF-κ-B (RANK) and carbonic anhydrase II (CA II) in ovariectomized rat osteoclast-like cells. Marrow cells were harvested from femora tibiae rats, which ovaries had been totally excised, cultured 6-well chamber slides. After 1 day incubation, marrow exposed PEMF for 3 days with 3.8 mT, 8 Hz, 40 min per day. Osteoclast-like confirmed by both tartrate resistant acid...
Maternal subclinical hypothyroidism (SCH) during pregnancy can adversely affect the neurodevelopment of offspring. The balance nerve growth factor (NGF)-related tropomyosin receptor kinase A/p75 neurotrophin (TrkA/p75NTR) signaling in hippocampus is important brain development, and whether it affects cognitive function maternal SCH's offspring not clear. In this study, we found that compared with control (CON) group, expression proliferation-related proteins [NGF, p-TrkA,...
Stimulation of α1-adrenoreceptors (α1-AR) acutely alters ion channel behavior via several signaling pathways [calcium and protein kinase C (PKC)]. Little is known about sustained α1-adrenergic/PKC regulation as may occur during cardiovascular disease states. Here we describe the effects prolonged α1A-AR PKC activity on human ether-a-go-go-related gene (HERG) K<sup>+</sup> channels (Kv11.1) expressed in a heterologous expression system. with phenylephrine or direct activation phorbol ester...
Objective Maternal hypothyroidism during pregnancy can affect the neurodevelopment of their offspring. This study aimed to investigate effects maternal subclinical (SCH) on spatial learning and memory, its relationship with apoptotic factors in cerebral cortex Methods Female adult Wistar rats were randomly divided into three groups ( n = 15 per group): control (CON) group, SCH group overt (OH) group. Spatial memory offspring evaluated by long-term potentiation (LTP) Morris water-maze (MWM)...
Objective: The neuroprotective effect of hypothermia has been well established. use for the treatment stroke by systemic is limited cooling rate and severe complications. goal this study was to determine if local cerebral via infusion could reduce infarction volume improve neurological outcome in a rat model middle artery (MCA) occlusion. Methods: A hollow filament used block MCA 2 hours, then ischemic territory locally infused with autocirculating cold arterial blood (13–15°C). This (<0.6...
BRAF gene mutation causes melanoma patients to develop drug resistance after 8-9 months inhibitors treatment. Therefore, overcoming inhibitor has important implications for improving patient survival. Sorafenib directly inhibits tumor cell proliferation by blocking the RAF/MEK/ERK-mediated signaling pathway. It remains unknown that whether combination of sorafenib with vemurafenib could sensitize cells vemurafenib, and underlying mechanism needs be clarified.Vemurafenib resistant A375/Vem...
Obstructive sleep apnea (OSA), a common breathing and sleeping disorder, is associated with broad range of neurocognitive difficulties. Intermittent hypoxia (IH), one major characteristic OSA, has been shown to impair learning memory due increased levels reactive oxygen species (ROS). Under normal conditions, ROS are produced in low concentrations act as signaling molecules different processes. However, IH treatment leads elevated production via multiple pathways, including mitochondrial...
Patients with mucoepidermoid carcinoma exhibit poor long-term prognosis because of the lack therapeutic strategies that effectively block tumor progression. We have previously characterized Ms cells as a highly metastatic cell line expresses high levels transforming growth factor β1 (TGF-β1). Here, we studied effect suppressing TGF-β1 by RNA silencing on invasive and potential carcinoma.Cell motility, substratum adhesion, transmembrane invasion were estimated migration, matrigel assay....