A5046835470- Pancreatic and Hepatic Oncology Research
- Genetic and Kidney Cyst Diseases
- Gastrointestinal disorders and treatments
- Pancreatic function and diabetes
- Renal and related cancers
- Growth Hormone and Insulin-like Growth Factors
- Congenital heart defects research
- Cancer, Hypoxia, and Metabolism
- Retinal Development and Disorders
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Phagocytosis and Immune Regulation
- Developmental Biology and Gene Regulation
- Caveolin-1 and cellular processes
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- TGF-β signaling in diseases
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Pituitary Gland Disorders and Treatments
- Complement system in diseases
- Inflammasome and immune disorders
- Inflammation biomarkers and pathways
ImmuNext (United States)
2020-2024
Dartmouth–Hitchcock Medical Center
2007-2023
Institute of Pharmacology
2023
Centre Hospitalier Universitaire de Toulouse
2020
Dartmouth College
2008-2016
Cotton (United States)
2012
Immunovaccine (Canada)
2009
Institut Cochin
2005
Inserm
2005
Centre National de la Recherche Scientifique
1993-2005
Abstract Primary cilia have been proposed to participate in the modulation of growth factor signaling pathways. In this study, we determined that ciliogenesis is suppressed both pancreatic cancer cells and intraepithelial neoplasia (PanIN) lesions human ductal adenocarcinoma (PDAC). were absent these even when not actively proliferating. Cilia also from mouse PanIN three different models PDAC driven by an endogenous oncogenic Kras allele. Inhibition effector pathways restored a cell line,...
Negative checkpoint regulators (NCRs) temper the T cell immune response to self-antigens and limit development of autoimmunity. Unlike all other NCRs that are expressed on activated lymphocytes, V-type immunoglobulin domain-containing suppressor activation (VISTA) is naïve cells. We report an unexpected heterogeneity within compartment in mice, where loss VISTA disrupted major quiescent subset enhanced self-reactivity. Agonistic engagement increased tolerance by promoting antigen-induced...
The essential roles of LIM homeodomain proteins in cell fate determination during development have been demonstrated organisms as divergent Drosophila and higher mammals. We isolated murine cDNAs encoding two highly homologous that specifically interact with the domains P-Lim/Lhx3 several other factors. Transcripts these factors can be detected early mouse E8.5, maximal expression observed regions embryo which P-Lim/Lhx3, Isl-1, LH-2 are selectively expressed. These potentiate...
Reciprocal gene activation and restriction during cell type differentiation from a common lineage is hallmark of mammalian organogenesis. A key question, then, whether critical transcriptional activator type–specific targets can also restrict expression the same genes in other types. Here, we show that whereas pituitary-specific POU domain factor Pit-1 activates growth hormone one type, somatotrope, it restricts its second lactotrope. This distinction depends on two–base pair spacing...
To determine the cell compartment in which initial oncogenic mutations occur pancreatic ductal adenocarcinoma (PDAC), we generated a mouse model endogenous expression of mutated Kras (Kras G12D ) was initially directed to population exocrine progenitors characterized by Nestin. Targeting such restricted sufficient for formation intraepithelial neoplasias (PanINs), putative precursors PDAC. PanINs appeared with same grade and frequency as observed when targeted whole pancreas Pdx1-driven Cre...
After differential screening of a cDNA library constructed from quail neuroretina cells infected with the v-myc containing avian retrovirus MC29, we have isolated clone Pax-QNR, homologous to murine Pax6 which is mutated in autosomal dominant mutation small eye (Sey) mouse and aniridia man. Here report characterization Pax-QNR/Pax-6 expression chicken, quail, pancreas. In situ hybridization performed E3 chick embryos demonstrated that, addition documented neural tube, this gene also...
After differential screening of a cDNA library constructed from quail neuroretina cells (QNR) infected with the v-myc-containing avian retrovirus MC29, we have isolated clone, Pax-QNR, homologous to murine Pax-6, which is mutated in autosomal dominant mutation small eye mice and disorder aniridia humans. Here report characterization Pax-QNR proteins expressed neuroretina. From bacterially peptides, obtained rabbit antisera directed against different domains protein: paired domain (serum 11),...
Targeting of oncogenic Kras to the pancreatic Nestin-expressing embryonic progenitor cells and subsequently adult acinar compartment is sufficient for development low grade intraepithelial neoplasia (PanIN) between 2 4 months. The mice die around 6 month-old unrelated causes, it therefore not possible assess whether lesions will progress carcinoma. We now report that two brief episodes caerulein-induced acute pancreatitis in causes rapid PanIN progression ductal adenocarcinoma (PDAC) by...
Enhancers have been functionally described for >35 years, but the molecular principles underlying integration of regulatory inputs to alternate gene enhancers used during mammalian organogenesis remain incompletely understood. Using a combination in vivo enhancer mapping and proteomics approaches, we established that two distant distinct early enhancers, each requiring different transcription complexes, are required full activation encoding pituitary lineage determining factor, Pit1. A...
Abstract Plasmalemma vesicle-associated protein (Plvap) is an endothelial with roles in diaphragm formation and maintenance of basal vascular permeability. At the same time, Plvap has immunity by facilitating leukocyte diapedesis at inflammatory sites controlling peripheral lymph node morphogenesis entry soluble Ags into conduits. Based on its postulated role diapedesis, we have investigated hematopoiesis show that deletion results a dramatic decrease IgM+IgDlo B cells both spleen peritoneal...
ACTH is frequently produced in non-pituitary tumours, leading to the ectopic-ACTH syndrome, but molecular mechanisms of its expression remain obscure. This study was aimed at understanding transcription ACTH-precursor gene carcinoid tumours lung or thymus.Transcripts coding for a series corticotroph-associated factor genes were detected, together with markers corticotroph phenotype. We studied 41 including 15 proven syndrome.Specific RT-PCR reactions designed each alternatively spliced...
PV1 is an endothelial-specific protein with structural roles in the formation of diaphragms endothelial cells normal vessels. also highly expressed on many solid tumours. On basis vitro data, thought to actively participate angiogenesis. To test whether or not has a function tumour angiogenesis and growth vivo, we have treated pancreatic tumour-bearing mice by single-dose intratumoural delivery lentiviruses encoding for two different shRNAs targeting murine PV1. We find that down-regulation...
Objectives: The present study was conducted to evaluate the expression and function of AP-2α isoforms in pancreatic ductal adenocarcinoma. Methods: evaluated at RNA level by reverse transcription-polymerase chain reaction protein Western blotting immunofluorescence. Its as a transcription factor transient transfection experiments: DNA binding properties electromobility shift assay transactivation capabilities luciferase assay. Results: Multiple alternative splicing events messenger occurred...