Dysbiosis in the intestinal microbiota of persons with inflammatory bowel disease (IBD) has been described, but there are still varied reports on changes abundance Bifidobacterium and Lactobacillus organisms patients IBD. The aim this investigation was to compare compositions mucosa-associated fecal bacteria IBD healthy controls (HCs). Fecal biopsy samples from 21 HCs, 15 Crohn's (CD) patients, 34 29 ulcerative colitis (UC) respectively, were analyzed by quantitative real-time PCR targeting...

Resuscitation-promoting factors (Rpfs), apparent peptidoglycan hydrolases, have been implicated in the reactivation of dormant bacteria. We previously demonstrated that deletion rpfB impaired Mycobacterium tuberculosis a mouse model. Because M. encodes five Rpf paralogues, redundant functions among family members might obscure rpf single-knockout phenotypes. A series double knockouts were therefore generated. One mutant, DeltarpfAB, exhibited several striking Consistent with proposed cell...

A major reason for the failure of advanced colorectal cancer (CRC) treatment is occurrence chemoresistance to oxaliplatin-based chemotherapy. Recently, studies have shown that long non-coding RNAs (lncRNAs) play an important role in drug resistance. Using HiSeq sequencing methods, we identified lncRNAs show differential expression levels oxaliplatin-resistant (OxR) and non-resistant CRC patients. RT-qPCR was then performed tissues serum samples, lncRNA MEG3 verified be downregulated...

Colorectal neuroendocrine tumors with liver metastases (CRNELM) are associated a poorer prognosis compared to their nonmetastatic counterparts. A comprehensive understanding of the tumor microenvironment (TME) heterogeneity between primary lesions (PL) and (LM) could provide crucial insights for enhancing clinical management strategies these patients. We utilized single-cell RNA sequencing analyze fresh tissue samples from CRNELM patients, aiming elucidate variations in TME PL LM....

This study aimed to investigate the role of JKAP in inflammatory bowel disease (IBD). expression was analyzed intestinal mucosa 81 IBD patients and 25 healthy controls (HCs) by qPCR immunoblotting. The correlations with clinical activity cytokines were performed. before after IFX treatment also measured. CD4+ T cells isolated from peripheral blood active patient HCs, transduced lentivirus-encoding (LV-JKAP), anti-JKAP (LV-anti-JKAP), or empty vector (LV-scramble), functions on subsequently...

Interleukin-23/T-helper 17 (IL-23/Th17) pathway plays a key role in the pathogenesis of inflammatory bowel disease (IBD), but little is known about its expression Chinese population. In this study, we investigated mRNA and protein levels IL-12p40, tumor necrosis factor-like cytokine 1A (TL1A), Janus kinase 2 (JAK2), IL-23R both locally systemically IBD patients. Our results indicated that IL-12p40 TL1A were increased ulcerative colitis (UC) Furthermore, serum higher active UC patients,...

Pancreatic cancer (PC), as the leading cause of death worldwide, is one deadliest tumors with a very low 5-year survival rate. Therefore, it urgent to seek new biomarkers PC for more accurate and reliable treatments. To identify differentially expressed miRNAs (DEM) in tissues, we performed systematic microarray qRT-PCR analyses. We found miR-196b was top dysregulated DEM tissues compared corresponding adjacent positively correlated poor differentiation, tumor size, lymphatic invasion TNM...

Background Natural killer T (NKT) cells share phenotypic and functional properties with both conventional natural cells. These might have an important role in the pathogenesis of ulcerative colitis (UC). The interaction chemokine ligand 25 (CCL25) receptor 9 (CCR9) is involved gut-specific migration leukocytes induces regulatory (Tregs) to migrate intestine chronic ileitis. Methodology/Findings In UC patients, NKT CD161, CCL25, CCR9 expression levels were evaluated by qRT-PCR. A murine model...

Background Genetic variants make some contributions to inflammatory bowel disease (IBD), including Crohn's (CD) and ulcerative colitis (UC). More than 100 susceptibility loci were identified in Western IBD studies, but gene has not been found Chinese patients till now. Sequencing of individuals with an family history is a powerful approach toward our understanding the genetics pathogenesis IBD. The aim this study, which focuses on Han CD family, identify high-risk potentially novel using...

Tumor necrosis factor (TNF) receptor-associated 5 (TRAF5) is a key mediator of TNF receptor superfamily members and important in both T helper (Th) cell immunity the regulation multiple signaling pathways. To clarify TRAF5’s influence on inflammatory bowel diseases (IBDs), we investigated TRAF5 deficiency’s effect dextran sulfate sodium- (DSS-) induced colitis. Colitis was knockout (KO) mice their wild-type (WT) littermates by administering 3% DSS orally for 7 days. The were then sacrificed,...

This study aimed to explore the role of CD4+ T cells in mechanisms COVID-19 related diarrhea.We analyzed lymphocyte subsets patients with and expression angiotensin-converting enzyme 2 (ACE2), transmembrane protease serine 2, cell-related indicators colon were compared between without diarrhea. Correlation analyses performed for ACE2 other identify relationship SARS-CoV-2 infection mediated inflammation. The distribution cell-associated chemokines their receptors detected determine...

Atherosclerosis is essentially the leading factor behind occurrences of cardiovascular diseases (CVDs)-associated incidents, while mitochondrial dysfunction also main cause atherosclerosis. The present study conducted a comparative analysis function-related indicators in cholesterol-induced vascular endothelial cells (VECs) from Mongolian gerbils, Sprague-Dawley (SD) rats and humans. It reported that inhibitory effect cholesterol treatment on viability gerbil VECs was markedly lower than...

The proinflammatory effects of IL12p40 had been documented in the literature, and anti-IL12p40 treatment proved to be effective therapy Crohn disease (CD) a phase 2b clinical trial. However, precise role pathogenesis inflammatory bowel (IBD) was still poorly understood. In this study, we investigated expressions its receptor interleukin-12 β 1 both locally systemically IBD cases healthy controls, contribution pathogenesis. We found that expression elevated at messenger RNA protein levels...

Infliximab is widely used in both inducing and maintaining remission of patients with Crohn’s disease (CD). The efficacy infliximab has been undoubtedly proven; however, various opportunistic infections have emerged. Herpes virus (being a type infection) CD treated alone no other concomitant medications are, rare not aroused enough attention. Gastroenterologists limited knowledge the immunization status CD, rarely do they take an adequate history before immunosuppressive therapy. Here we...

In this paper, the momentum and heat transfer between charged particles neutral in a needle-to-plane dielectric barrier discharge reactor with Ar as working gas are simulated. simulation, from ions to atom is described by interaction term atoms. Detailed reaction mechanisms considered simulation including metastable atom. The Navier-Stokes equation solved combination Poisson drift-diffusion equations. interrelation flow characteristics preliminary revealed. particular, when streamer strikes...

There is increasing evidence that several genes are associated with an increased risk of type 2 diabetes (T2D); genome-wide association investigations and whole-genome re‑sequencing offer a useful approach for the identification involved in common human diseases. To further investigate which polymorphisms confer susceptibility to T2D, present study screened high‑contribution gene variants Chinese patients T2D using whole‑genome DNA pooling. In total, 100 individuals healthy were analyzed...

Background: The strategy of specifically depleting antigen-specific T cells can potentially be used for the treatment allograft rejection and autoimmunity because it does not suppress overall immune systems.Methods: In this study, we generated killer polylactic-co-glycolic acid (PLGA) microspheres by covalently coupling major histocompatibility complex (MHC) class I antigens apoptosis-inducing anti-Fas monoclonal antibody (mAb) onto PLGA microspheres. A modified double-emulsion method was...

Growing evidence has shown that coagulation processes play an important role in disease pathogenesis and/or progression patients with inflammatory bowel disease. However, no study ever focused on the possible influence of infliximab (IFX) therapy status Crohn's (CD).To investigate difference biomarkers between CD and control participants, evaluate impact IFX usage patients.A retrospective included a case-control self-control was designed. The medical records participants were evaluated...