A5048993419- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cancer Mechanisms and Therapy
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Liver physiology and pathology
- Ferroptosis and cancer prognosis
- Gastric Cancer Management and Outcomes
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Ocular Oncology and Treatments
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Autophagy in Disease and Therapy
- Galectins and Cancer Biology
- Cancer Immunotherapy and Biomarkers
- Advanced Glycation End Products research
- Pediatric Hepatobiliary Diseases and Treatments
- TGF-β signaling in diseases
Wuhan University
2016-2024
Zhongnan Hospital of Wuhan University
2016-2024
Hubei Provincial Center for Disease Control and Prevention
2016-2024
Yanbian University Hospital
2024
Tianjin Central Hospital of Gynecology Obstetrics
2021-2024
Jilin University
2023
First People's Hospital of Chongqing
2022
Tianjin University
2021
Nankai University
2021
ORCID
2020
MiR-93 was observed in various types of cancers. This study is to investigate a role miR-93 the carcinogenesis HCC.The expression HepG2 cells and prima-ry human hepatocytes (PHHC) measured by RT-PCR. were transfected with inhibitor or negative control. The cell proliferation determined using CellTiter 96® Aqueous One Solution Cell Proliferation Assay kit. migration clonogenicity vitro assay, colony formation analysis anchorage-in-dependent growth assay. apoptosis cycle detected flow...
MicroRNAs (miRNAs) are believed to have fundamental roles in tumorigenesis and great potential for the diagnosis treatment of cancer. However, miRNAs hepatocellular carcinogenesis still not fully elucidated. We investigated aberrantly expressed involved hepatoma by comparison miRNA expression profiles cancerous hepatocytes with normal primary human hepatocytes, 37 dysregulated were screened out twofold change a significant difference (P<0.05). Clustering analysis based on 13 changes over...
Hypoxia is often found in solid tumors and associated with tumor progression poor clinical outcomes. The exact mechanisms related to hypoxia-induced invasion metastasis remain unclear. We elucidated the mechanism by which nuclear-damage–associated molecular pattern molecule, high-mobility group box 1 (HMGB1), released under hypoxic stress, can induce an inflammatory response promote hepatocellular carcinoma (HCC) cells. Caspase-1 activation was occur HCC cells a process that dependent on...
Abstract Posttranslational modifications increase the complexity and functional diversity of proteins in response to complex external stimuli internal changes. Among these, protein lipidations which refer lipid attachment are prominent, primarily encompassing five types including S-palmitoylation, N-myristoylation, S-prenylation, glycosylphosphatidylinositol (GPI) anchor cholesterylation. Lipid plays an essential role regulation trafficking, localisation, stability, conformation,...
// Jing-Jun Yan 1, 2 , Yu-Nan Zhang 1 Jia-Zhi Liao Kun-peng Ke 3 Ying Chang Pei-Yuan Li Min Wang Ju-Sheng Lin Xing-Xing He Institute of Liver Diseases, Tongji Hospital, Medical College, Huazhong University Science and Technology, Wuhan 430030, China Department Emergency Medicine, Cardiac Surgery, Asia Heart 430022, Correspondence to: He, e-mail: xxhe@tjh.tjmu.edu.cn Keywords: hepatocellular carcinoma, microRNA, tumor biology, cancer Received: April 01, 2015 Accepted: August 10, Published:...
Resistance to chemotherapy is a major challenge for the treatment of patients with colorectal cancer (CRC). Previous studies have found that microRNAs (miRNAs) play key roles in drug resistance; however, role miRNA-373-3p (miR-375-3p) CRC remains unclear. The current study aimed explore potential function miR-375-3p 5-fluorouracil (5-FU) resistance. MicroRNA-375-3p was be widely downregulated human cell lines and tissues promote sensitivity cells 5-FU by inducing colon apoptosis cycle arrest...
Abstract Background Hepatocellular carcinoma (HCC) is one of the most prevalent and inflammation-associated cancers. The tumor microenvironment (TME) plays an essential role in HCC development metastasis, leading to poor prognosis. The overall TME immune cells infiltration characterizations mediated by immune-related genes (IRGs) remain unclear. In this study, we aimed investigate whether could be indicators for prognosis patients cell characterization as well responses immunotherapy....
Abstract Liver inflammation plays a critical role in hepatocellular carcinoma (HCC) etiology. Damage-associated molecular patterns (DAMP), such as high-mobility group box 1 (HMGB1), and dysregulated miRNAs involved inflammatory disease states, miR-21, may participate the link between cancer. We sought to determine of HMGB1 signaling HCC tumor progression. first document concordant expression increase miR-21 cell lines primary samples subsequently show that stimulation results overexpression...
Emerging evidence indicates that microRNAs (miRNAs) are often dysregulated and play a fundamental role in hepatocellular carcinoma (HCC). However, the mechanism underlying miRNA dysregulation is still elusive. In present study, we adopted an integrated analysis strategy combining data from genome-wide methylated DNA immunoprecipitation chip expression microarray to study regulation of methylation on HCC. We first characterized 864 differentially regions (DMRs) located 236 between cancerous...
Growing tumor cells possess a distinct metabolic phenomenon that allows them to preferentially utilize glucose through aerobic glycolysis, which is referred as the "Warburg effect." Accumulating evidence suggests microRNAs (miRNAs) could regulate such reprogramming. Our microarray analysis and quantitative real-time PCR validation revealed miR-885-5p was strongly downregulated in hepatocellular carcinoma (HCC) tissues cell lines. To investigate miR-885-5p's biological functions HCC...
// Pengfei Chen 1, 2, 3, * , Fan Wang Juerong Feng 2 Rui Zhou Ying Chang Jing Liu and Qiu Zhao 1 Department of Gastroenterology, Zhongnan Hospital Wuhan University, Wuhan, China Hubei Clinical Center Key Laboratory Intestinal Colorectal Diseases, 3 The Central Enshi Autonomous Prefecture, Enshi, These authors contributed equally to this work Correspondence to: Zhao, email: zhaoqiuwhu@163.com Keywords: hepatocellular carcinoma, co-expression network analysis, hub genes, metastasis risk,...
Podocyte damage is strongly associated with the progression of diabetic nephropathy. Mitotic catastrophe plays an essential role in accelerating podocyte loss and detachment from glomerular basement membrane. In current study, we observed that long non-coding RNA (lncRNA) MIAT was noticeably upregulated plasma kidney tissues patients nephropathy, this upregulation accompanied by higher albumin/creatinine ratios serum creatinine levels. By generating CRISPR-Cas9 Miat-knockout (KO) mice vivo...
microRNAs (miRNAs) are negative regulators of gene expression and can function as tumor suppressors or oncogenes. Several miRNAs associated with the development hepatocellular carcinoma (HCC). miR-96 has been closely cell proliferation clonogenicity. Upregulation observed in various types cancer. However, biological hepatocarcinogenesis remains largely unknown. In this study, we demonstrated that was upregulated HCC inhibition significantly suppressed colony formation. The levels forkhead...
Hepatocellular carcinoma (HCC) is a worldwide malignance, and the underlying mechanisms of this disease are not fully elucidated. In study, existence function achaete-scute homolog-1 (ASH1)-miR-375-YWHAZ signaling axis in HCC were determined. Our experiments Cancer Genome Atlas (TCGA) sequencing data analyses showed that ASH1 miR-375 significantly downregulated, whereas YWHAZ was upregulated HCC. Furthermore, we found positively regulates miR-375, directly downregulates its target YWHAZ....
Hepatocellular carcinoma (HCC) remains a major global health burden due to its high prevalence and mortality. Emerging evidence reveals that microRNA (miRNA) plays vital role in cancer pathogenesis is widely involved the regulation of signaling pathways via their targeting downstream genes. MiR-21-3p, liver-enriched miRNA, SMAD7, negative regulator TGF-β pathway, likely exert influence on HCC progression.Here, we explore miR-21-3p-SMAD7/YAP1 axis pathogenesis.MiRNA microarray analysis was...
Background Human leukocyte antigen DP (HLA-DP) locus has been reported to be associated with hepatitis B virus (HBV) infection in populations of Japan and Thailand. We aimed examine whether the association can replicated Han Chinese populations. Methodology/Principal Findings Two HLA-DP variants rs2395309 rs9277535 (the most strongly SNPs from each locus) were genotyped three independent cohorts consisting 2 805 cases 1 796 controls. By using logistic regression analysis, these two HLA-DPA1...
Hepatocellular carcinoma (HCC) is a worldwide malignancy; however, there lack of effective targeted therapies. We and others have found that miR-221 one the most consistently overexpressed miRNAs in liver cancer. However, roles hepatocellular carcinogenesis are still not fully elucidated. In present study, we used bioinformatics tools, gain- loss-of-function methods to determine HCC. Bioinformatics analysis showed core miRNA which targets large number HCC-related genes has formed many...
This study aimed to investigate the role of JKAP in inflammatory bowel disease (IBD). expression was analyzed intestinal mucosa 81 IBD patients and 25 healthy controls (HCs) by qPCR immunoblotting. The correlations with clinical activity cytokines were performed. before after IFX treatment also measured. CD4+ T cells isolated from peripheral blood active patient HCs, transduced lentivirus-encoding (LV-JKAP), anti-JKAP (LV-anti-JKAP), or empty vector (LV-scramble), functions on subsequently...
Colon cancer stem cells (CSCs) have been shown to be responsible for the recurrence and metastasis of colorectal (CRC). As a crucial microenvironmental factor, extracellular matrix (ECM) stiffness is known affect stemness CSCs. Recently, fibrin deposition in stroma CRC was demonstrated tumor development. In this study, we used salmon gel provide 3D ECM colon investigated its effects on cell growth as well underlying mechanisms. Compared with stiff 420 Pascal (Pa) 1 050 Pa gels, 90 soft most...
Emerging evidence has indicated that microRNAs (miRNAs) are frequently dysregulated and fundamental in the pathogenesis of hepatocellular carcinoma (HCC). However, roles miR-195 HCC have not been well elucidated. In present study, expression miR‑195 was determined to be markedly downregulated tissues cell lines, as compared with normal liver cells. Restoration resulted significant inhibition proliferation tumorigenicity cells vitro vivo. Gene data luciferase reporter assays revealed is able...