The incorporation of novel, functional, and sustainable foods in human diets is increasing because their beneficial effects environmental-friendly nature. Chia (Salvia hispanica L.) has proved to be a suitable source bioactive peptides via enzymatic hydrolysis. These could responsible for modulating several physiological processes if able reach the target organ. bioavailable contained hydrolysate obtained with Alcalase, as functional foods, were identified using transwell system Caco-2 cell...

The KDEL receptor is a Golgi/intermediate compartment-located integral membrane protein that carries out the retrieval of escaped ER proteins bearing C-terminal sequence. This occurs throughout retrograde traffic mediated by COPI-coated transport carriers. role cytoplasmic domain in this process has been investigated. Deletion did not affect subcellular localization although cells expressing truncated form failed to retain ligands intracellularly. Permeabilized incubated with ATP and GTP...

Hemp is the term commonly used to refer variety of Cannabis sativa L. cultivated for industrial purposes. The seeds have gained interest in recent years as functional foods due their nutritional composition and high content protein bioactive compounds. In this study, ten hemp hydrolysates (HPHs) were obtained by enzymatic hydrolysis with Alcalase Flavourzyme from isolate (HPI) antioxidant properties (DPPH radical scavenging activity, beta-carotene activity ferric ion reducing power (FRAP))...

We have examined the role played by protein kinase A (PKA) in vesicle-mediated transport from trans-Golgi network (TGN) to cell surface. In vivo this step was inhibited inhibitors of PKA catalytic subunits (C-PKA) such as compound known H89 and a myristoylated form inhibitory peptide sequence contained thermostable inhibitor. Inhibition occurred at an early stage during transfer vesicular stomatitis virus G glycoprotein TGN Reversal inhibition correlated with transient increase number free...

An alternative approach for cell-culture end-point protocols is proposed herein. This new technique suitable real-time remote sensing. It based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test (OBT) method. Simple straightforward circuit blocks form basis of measurement system. Oscillation parameters - frequency amplitude constitute outcome, directly correlated with culture status. A user can remotely track evolution cell cultures in real time...

Agri-food industries generate several by-products, including protein-rich materials currently treated as waste. Lupine species could be a sustainable alternative source of protein compared to other crops such soybean or chickpea. Protein hydrolysates contain bioactive peptides that may act positively in disease prevention treatment. Inflammatory responses and oxidative stress underlie many chronic pathologies natural treatment approaches have gained attention an synthetic pharmaceuticals....

Seeds from non-drug varieties of hemp (Cannabis sativa L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh monocytes macrophages derived circulating were to evaluate both plasticity effects HSO at 10–100 mM using FACS analysis, ELISA, RT-qPCR methods. The quantitative the acyclic alcohol fraction isolated is most abundant component (167.59 ± 1.81...

Sacha inchi (Plukenetia volubilis L.) is an under-exploited crop with great potential due to its nutritional and medicinal characteristics. A protein isolate (SII), obtained from defatted flour (SIF), was hydrolyzed by Bioprotease LA 660 under specific conditions. The hydrolysates were characterized chemically, their digestibility antioxidant capacity evaluated in vitro cell-free experiments select the hydrolysate major activity. at 20 min (SIH20B) selected, anti-inflammatory RT-qPCR ELISA...

The effect of unsaturated and saturated nonesterified fatty acids (NEFAs) on the electrophoretic, immunological, steroid-binding properties human sex hormone-binding protein (SBP) were investigated. Tests carried out whole serum from pregnant women purified SBP using polyacrylamide gel electrophoresis, crossed immunoelectrophoresis with autoradiography, equilibrium dialysis. All three methods showed that NEFAs influence binding steroids to both in protein. Saturated caused a 1.5-2-fold...

Grape seed oil has significant potential for the management of inflammatory and oxidative conditions.

The increase in the world population along with new policies aimed at a more sustainable has led to need of searching for food sources, which are environmentally friendly, implying healthy and nutritious diets. This study explored biological activity two kiwicha (Amaranthus caudatus L.) protein hydrolysates obtained aid Bioprotease LA-660 regarding their anti-inflammatory response intestinal level, employing CACO-2 cell line. results showed that vitro administration these decreased...

Auditory nerve and brain stem evoked responses were summated from multiple scalp earlobe electrodes five subjects with normal hearing to determine the spatial voltage relationships along coronal and/or sagital planes that make up electrode array. All recordings maintained their positive polarity, polarity inversions not observed at any cephalic sites; thus, a null potential line was identified for these short-latency potentials scalp. Less distinct derived earlobes when referenced chin....

The small GTP-binding protein ADP-ribosylation factor 1 (ARF1) is an essential component of the molecular machinery that catalyzes formation membrane-bound transport intermediates. By using in vitro assay reproduces recruitment cytosolic proteins onto purified, high salt-washed Golgi membranes, we have analyzed role cAMP-dependent kinase A (PKA) on ARF1 incorporation. Addition to this either pure catalytic subunits PKA (C-PKA) or cAMP increased binding. contrast, association was inhibited...

We have analyzed the effects of minor compounds found in unsaponifiable fraction (UF) and phenolic (PF) virgin olive oil (VOO) on LPS-induced inflammatory response via visfatin modulation human monocytes. For this purpose, monocytes were incubated with UF PF at different concentrations pro-inflammatory stimulus LPS for 24 hr; squalene (SQ) hydroxytyrosol (HTyr), main components PF, respectively, also used. The relative expression both anti-inflammatory genes, as well other genes related to...

Proteins binding to the PEA3 enhancer motif (AGGAAG) activate polyomavirus early promoter and help comprise viral late mRNA initiator element (W. Yoo, M. E. Martin, W. R. Folk, J. Virol. 65:5391-5400, 1991). Because many developmentally regulated cellular genes have motifs near their sequences, because Ets family gene products motif, we studied expression of PEA3-binding proteins Ets-related during differentiation F9 embryonal carcinoma cells. An approximately 91-kDa protein (PEA3-91) was...

Dietary fatty acids have been demonstrated to modulate systemic inflammation and induce the postprandial inflammatory response of circulating immune cells. We hypothesized that triglyceride-rich lipoproteins (TRLs) may acute effects on immunometabolic homeostasis by modulating dendritic cells (DCs), sentinels immunity link innate adaptive systems. In healthy volunteers, saturated acid (SFA)-enriched meal raised serum levels granulocyte/macrophage colony-stimulating factor GM-CSF (SFAs >...

ABSTRACT The Golgi complex represents a major subcellular location of protein kinase A (PKA) concentration in mammalian cells where it has been previously shown to be involved vesicle-mediated transport processes. We have studied the factors that influence interaction PKA typeII subunits with complex. In addition cytosol, both catalytic (Cα) and regulatory (RIIα) PKAII were detected at sides stack, particularly elements cis- trans-Golgi networks. subunits, contrast, practically absent from...

This paper proposes a new yet efficient method allowing significant improvement in the on-line analysis of biological cell growing and evolution. The procedure is based on an empirical-mathematical approach for calibration fitting any cell-electrode electrical model. It valid can be extrapolated type cellular line used cell-substrate impedance spectroscopy (ECIS) tests. Parameters bioimpedance model, acquired from ECIS experiments, vary each line, which makes obtaining results difficult...