A5068181497- Diabetes and associated disorders
- Pancreatic function and diabetes
- Blood Coagulation and Thrombosis Mechanisms
- Glycosylation and Glycoproteins Research
- Hematopoietic Stem Cell Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Retinal Diseases and Treatments
- Chronic Myeloid Leukemia Treatments
- Systemic Lupus Erythematosus Research
- Blood disorders and treatments
- Atherosclerosis and Cardiovascular Diseases
- Adipose Tissue and Metabolism
- Erythrocyte Function and Pathophysiology
- Growth Hormone and Insulin-like Growth Factors
- Cellular Mechanics and Interactions
- Hemophilia Treatment and Research
- Platelet Disorders and Treatments
- Immune Response and Inflammation
- Hippo pathway signaling and YAP/TAZ
- Cell Adhesion Molecules Research
- Cardiomyopathy and Myosin Studies
- Kruppel-like factors research
- Diabetes Management and Research
- Angiogenesis and VEGF in Cancer
Cincinnati Children's Hospital Medical Center
2014-2025
University of Cincinnati
2015-2025
University of Cincinnati Medical Center
2013-2024
Manmohan Memorial Institute of Health Sciences
2024
Janaki Medical College
2017
Laboratoire d’immunologie intégrative du cancer
2015
The University of Texas Health Science Center at Tyler
2008-2013
Central Drug Research Institute
2005-2009
University of Arizona
2004-2007
Tufts University
1997-2007
Abstract We determined whether human NK cells could contribute to immune defenses against Mycobacterium tuberculosis through production of IL-22. CD3−CD56+ produced IL-22 when exposed autologous monocytes and γ-irradiated M. tuberculosis, this depended on the presence IL-15 IL-23, but not IL-12 or IL-18. IL-15-stimulated expressed 10.6 times more DAP10 mRNA compared with control cells, siRNA inhibited IL-15-mediated by cells. Soluble factors IL-15-activated growth in macrophages, effect was...
The identification of microvascular pericytes in vitro relies principally on morphological characteristics and growth dynamics, as there is a paucity immunochemical markers for these cells. Consequently, an attempt was made to identify mAb reagents that would aid both the rapid enrichment retinal capillary vascular cell cultures. A panel mAbs raised by xenogeneic immunization mice with various tissues screened immunoreactivity dissociated cultures bovine pericytes. Two antibodies from (3G5...
We have biochemically treated (periodate, borohydride, neuraminidase, organic solvents) frozen sections of human pancreas and studied the reactivity islet-cellantibody-positive sera monoclonal antibodies. The autoantigen pancreatic has properties ofsialic acid containing glycolipid.
Perivascular wall tumors (PWTs) are defined as neoplasms deriving from mural cells of blood vessels, excluding the endothelial lining. The spectrum human cutaneous PWT includes glomus tumor, hemangiopericytoma (HEP), myopericytoma, angioleiomyoma/sarcoma, angiomyofibroblastoma, and angiofibroma. purpose this study was to revise clinical presentation, cytology, histopathology, immunohistology canine with cytology typical HEP. Diagnosis established on basis vascular growth patterns (staghorn,...
Multiple sclerosis (MS) is assumed to be an autoimmune disease initiated by autoreactive T cells that recognize central nervous system antigens. Although adaptive immunity clearly involved in MS pathogenesis, innate increasingly appears implicated the disease. We and others have presented evidence natural killer (NK) may immunoregulation MS, leading question of whether a particular NK cell subtype will account for this effect. Changes functionality were associated with activity, depletion...
Abstract Human bone marrow stromal cells (BMSCs, also known as marrow-derived mesenchymal stem cells) are manufactured using many different methods, but little is about the spectrum of manufacturing methods used and their effects on BMSC characteristics function. Seven centers using, one developing, Good Manufacturing Practices (GMP) processes were surveyed to production methods. Among seven centers, all aspirates starting material, no two same Two four lots from each center compared global...
Severe congenital neutropenia (SCN) is often associated with inherited heterozygous point mutations in ELANE, which encodes neutrophil elastase (NE). However, a lack of appropriate models to recapitulate SCN has substantially hampered the understanding genetic etiology and pathobiology this disease. To end, we generated both normal patient-derived induced pluripotent stem cells (iPSCs), performed genome editing differentiation protocols that major features granulopoiesis. Pathogenesis ELANE...
Adult rat ventricular myocytes undergo a well-documented sequence of phenotypic changes during adaptation to primary culture. However, we observed that coculture with specific subset nonmyocyte cardiac cells could slow and even reverse the process adaptation. These were isolated identified by immunohistochemical ultrastructural criteria as being epicardial mesothelial origin. When added long-term cultures adult myocytes, appeared induce myofibrillar arrays more organized than those seen in...
With biochemical and enzymatic treatment of frozen sections pancreas, we have previously shown that cytoplasmic islet cell antibodies (ICAs) react with carbohydrate determinants glycoconjugates. As a first step toward purifying these glycoconjugates, human pancreas tissue was extracted in mixture chloroform methanol, the glycolipids were obtained by effecting Folch partition. The protein pellet, lipid fraction, glycolipid fraction so assessed for their ability to block binding ICAs effect...
In the bone marrow (BM), hematopoietic progenitors (HPs) reside in specific anatomical niches near osteoblasts (Obs), macrophages (MΦs), and other cells forming BM microenvironment. A connection between immunosurveillance traffic of HP has been demonstrated, but regulatory signals that instruct immune regulation circulation are unknown. We discovered microenvironment deficiency p62, an autophagy regulator signal organizer, results loss autophagic repression macrophage contact-dependent...
Abstract Bacterial and fungal infections are a major cause of morbidity mortality in neutropenic patients. Donor-derived neutrophil transfusions have been used for prophylaxis or treatment infection However, the short half-life limited availability large numbers donor-derived neutrophils transfusion remain significant hurdle implementation therapy. Here, we investigate vitro vivo activity generated from human induced pluripotent stem cells (iPSC), potentially unlimited resource to produce...
Abstract Background Chromatin remodeler chromodomain helicase DNA-binding protein 8 (CHD8) defines a subtype of autism that is associated with immune disorders. It remains unknown whether CHD8 plays cell-intrinsic role in cells such as regulatory T (Tregs) maintain tolerance through suppressing CD4 + and CD8 effector cells. Methods Treg-specific conditional CHD8-deficient mice were generated by crossing Chd8 Flox/Flox Foxp3 YFP−cre transgenic mice. Effects deficiency investigated using...
Abstract Background : The immunogold labeling technique and transmission electron microscopy were used to demonstrate the expression position of intermediate filament vimentin in rat osteoblast osteocyte cell bodies processes. Conventional light microscopic studies bone cells demonstrated adjacent linkage be mediated by processes present within canalicular system traversing matrix. filled with densely packed filaments, many which have been shown previously actin microfilaments. appearance,...
gamma delta T-cell receptor-bearing T cells (gamma cells) are readily activated by intracellular bacterial pathogens such as Mycobacterium tuberculosis. The antigens responsible for activation remain poorly characterized. We have found that heat treatment of live M. tuberculosis bacilli released into the supernatant an antigen which stimulated human cells. was measured determining increase in percentage flow cytometry peripheral blood mononuclear with and proliferation lines monocytes...
Recent biochemical studies have shown that the cytoplasmic islet cell–antibody autoantigen has properties of a monosialoganglioside (GM). To characterize glycolipids and ascertain whether islets express unique gangliosides, we determined pattern ganglioside expression in whole human pancreas isolated using high-performance liquid chromatography (HPLC) thin-layer (HPTLC). The major gangliosides detected glycolipid extracts were GM3, GD3 (disialoganglioside), lesser amount, GD1a-comigrating...
The extrapancreatic actions of sulfonylureas on the glucose transport system were studied in L6 line cultured rat skeletal muscle cells. Insulin (10(-7) M) increased 2-deoxyglucose uptake differentiated myotubes by 30-40% after 8 h incubation. sulfonylurea tolazamide (0.6 mg/ml, 22 h) had no effect absence insulin, but insulin-stimulated twofold. total cellular content transporters was assessed with a monoclonal anti-transporter antibody solid-phase ELISA method. (8 quantity transporters,...
Whereas actin-binding proteins (ABPs) regulate network formation during the cell cycle, it is not known whether ABPs also function to sequester or target isoactins specific subcellular compartments. Recently, we have shown that ezrin indirectly associates with β, but α actin filaments in a calcium- and cytochalasin-sensitive manner [Shuster Herman, 1995: J. Cell Biol. 128:837–848]. To identify β actin-specific binding protein fosters ezrin-β interactions, developed an isoactin affinity...