A5100777213- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Animal Genetics and Reproduction
- Nuclear Structure and Function
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Toxin Mechanisms and Immunotoxins
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Neurogenesis and neuroplasticity mechanisms
- Enzyme Structure and Function
- Cancer-related Molecular Pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Multilevel Inverters and Converters
- Liver physiology and pathology
- Protein purification and stability
- Wnt/β-catenin signaling in development and cancer
- Steroid Chemistry and Biochemistry
- Tissue Engineering and Regenerative Medicine
- Transgenic Plants and Applications
Beijing Jiaotong University
2012-2024
Institute of Animal Sciences
2011
Chinese Academy of Agricultural Sciences
2011
Cellular respiration is a worthwhile criterion to evaluate mitochondrial dysfunction by measuring the dissolved oxygen. However, most of existing sensing strategies merely report extracellular (ec-) or intracellular (ic-) O2 rather than intramitochondrial (im-) . Herein we present method assess tumor with three phosphorescent nanosensors, which respond ec-, ic-, and im-O2 Time-resolved luminescence applied determine respective oxygen consumption rates (OCRs) under varying respiratory...
Mesenchymal stem cells (MSCs), with their capacity for self-renewal and differentiation into various cell types, are important seed therapy. MSCs exhibit potent pathotropic migratory properties that make them attractive use in tumor prevention However, little is known about the underlying molecular mechanisms link to targeted cells. This study investigated inhibitory effect mechanism of on human hepatoma HepG2 using co-culture conditioned medium system animal transplantation model. The were...
Abstract Background Endothelial progenitor cells (EPC) are a type of stem cell used in the treatment atherosclerosis, vascular injury and regeneration. At present, most EPCs studied from human mouse, whereas study poultry-derived has rarely been reported. In present study, chicken bone marrow-derived were isolated at cellular level using immunofluorescence RT-PCR. Results We found that majority spindle shaped. The growth-curves passages (P) 1, -5 -9 typically “S”-shaped. viability EPCs,...
Sma mothers against decapentaplegic homologue 4 (Smad4) is a key mediator of the transforming growth factor β (TGF-β) pathway and plays complex contradictory roles in hepatocellular carcinoma (HCC). However, specific role Smad4 hepatocytes regulating hepatocarcinogenesis remains poorly elucidated.
Skeletal muscle satellite cells, a postulated multipotential stem cell population, play an essential role in the postnatal replenishment of skeletal muscles. In present research, cells were isolated from pectorals 15-day-old Beijing Fatty Chicken embryos using combined enzymatic digestion 0.1% collagenase 1 and 0.25% trypsin. Myogenic markers such as MyoD, Pax7 demin detected, indicating their identity. Karyotype analysis showed that these vitro cultured genetically stable. Being exposed to...
Embryonic germ (EG) cells derived from primordial are undifferentiated and pluripotent stem cells. In our study, isolated the genital ridges of Beijing duck (Anas domestica) embryo at stage 28 were co-cultured with mice embryonic fibroblasts. Duck EG grew a high nuclear:cytoplasm ratio positive for periodic acid-Schiff alkaline phosphatase staining. The could proliferate more than month in repeated subculture maintain diploid karyotype. They expressed markers such as stage-specific...
The aim of this study was to explore the isolation and culture process Beijing fatty chicken primordial germ cells (PGCs) investigate their biological characteristics. PGCs isolated from genital ridges (Gallus domesticus) embryos after 5.5 days incubation were co-cultured with mice embryonic fibroblasts (MEF). results showed that positive for periodic acid Schiff (PAS) alkaline phosphatase (AKP) staining. These could proliferate a prolonged time in vitro maintain diploid karyotype....
Interleukin-24 (IL-24) displays tumor cell-specific proliferation inhibition in vitro and vivo. Recombinant human IL-24 (rhIL-24) has significantly higher activity, yet lower expression level mammalian cells than bacteria. To further realize therapeutic potential of IL-24, we enhanced rhIL-24 cell systems by adapting engineered Flp-InTMCHO/IL-24 (FCHO/IL-24) (adherent cultured Ham's F12 medium with 10% serum) to serum-free suspension culture. First, MTT assay showed that among four different...
DEK is a protein ubiquitously expressed in multicellular organisms as well certain unicellular organisms. It associated with the regulation of cell proliferation, differentiation, migration, apoptosis, senescence, self-renewal and DNA repairing. In tumor cells it carcinogenesis process, however there have been few previous studies into expression lung cancer. present study level mRNA was detected cancer tissues non-cancerous counterparts by performing reverse transcription-quantitative...
Interleukin-24 (IL-24) has specific inhibitory effects on the proliferation of various tumor cells with almost no toxicity to normal cells. The antitumor activity recombinant human IL-24 protein produced in mammalian is much higher than that bacteria, but its expression level extremely low. Sodium butyrate (NaBu) was utilized as a media additive increase Chinese hamster ovary site-specific integrated engineered FCHO/IL-24 were treated NaBu under different culture conditions (10% and 0.5%...
This study endeavors to analyze the effects of miR-1204 on expression DEK oncogene in non-small cell lung cancer (NSCLC) lines and molecular mechanisms these effects.The mimics inhibitors were transfected into (A549 SPC) NSCLC cells. Then mRNA levels, viability, apoptosis rate, morphology caspase activity determined. The apoptosis-related proteins Bcl-2 Bax was also analyzed.In SPC), levels down-regulated overex-pression group. In inhibition group, showed an opposite trend. overexpression...
Both embryonic and adult neural stem cells (NSC) of rodents humans have been isolated cultured in vitro to date, they are thought tremendous clinical promise restoring the diseased or injured central nervous system. However, there few counterpart reports on from birds. This study explored isolation culture system duck progenitor (NSPC) investigated their major biological properties. Cells dorsal ventricular ridges 10- 13-d embryos were isolated, cultured, purified by using a neurosphere...
Abstract Objectives: Many kinds of cardiac progenitor cell populations have been identified, including c‐kit + , Nkx2.5 s and GATA 4 cells. However, these progenitors limited ability to differentiate into different types. Recently, a new kind named the multipotent Isl1 cardiovascular ( MICP s) has which also expresses Nkx2.5, 4, CD 34 Flk1. Materials methods: In this study, we isolated characterized from chicken embryonic heart tissues using immunofluorescence PCR. Results: Results shown...
In this current work, two new-style DOPO-containing benzoxazines based on polyether-urea were successfully synthesized by a modified version via three-step procedures, and the corresponding polybenzoxazines with high toughness flame retardancy obtained thermal activated polymerization. The single double named as BzD1 BzD2, respectively. polymers followed PBzD1 PBzD2, curing behaviors of new investigated differential scanning calorimetry. mechanical properties materials evaluated TGA dynamic...