A5026893905- Neuroendocrine regulation and behavior
- Viral Infections and Outbreaks Research
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Olfactory and Sensory Function Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- Streptococcal Infections and Treatments
- Biochemical Analysis and Sensing Techniques
- Spinal Cord Injury Research
- Blood Coagulation and Thrombosis Mechanisms
- Stress Responses and Cortisol
- Lung Cancer Research Studies
- Plant Virus Research Studies
- Rabies epidemiology and control
- Venomous Animal Envenomation and Studies
- Nerve injury and regeneration
- Functional Brain Connectivity Studies
- Transcranial Magnetic Stimulation Studies
- Mosquito-borne diseases and control
- Adipose Tissue and Metabolism
- Virology and Viral Diseases
Ludwig-Maximilians-Universität München
2016-2024
The insular cortex (IC) plays key roles in emotional and regulatory brain functions is affected across psychiatric diseases. However, the brain-wide connections of mouse IC have not been comprehensively mapped. Here, we traced whole-brain inputs outputs its rostro-caudal extent. We employed cell-type-specific monosynaptic rabies virus tracings to characterize afferent onto either excitatory or inhibitory neurons, adeno-associated viral label efferent axons. While connectivity between other...
Vaccines of outstanding efficiency, safety, and public acceptance are needed to halt the current SARS-CoV-2 pandemic. Concerns include potential side effects caused by antigen itself safety viral DNA RNA delivery vectors. The large spike (S) protein is main target COVID-19 vaccine candidates but can induce non-neutralizing antibodies, which might cause vaccination-induced complications or enhancement disease. Besides, encoding a functional S in replication-competent virus vector vaccines may...
Abstract CD4 + T cells are central mediators of adaptive and innate immune responses constitute a major reservoir for human immunodeficiency virus (HIV) in vivo. Detailed investigations resting have been precluded by the absence efficient approaches genetic manipulation limiting our understanding HIV replication restricting efforts to find cure. Here we report method rapid, efficient, activation-neutral gene editing resting, polyclonal using optimized cell cultivation nucleofection...
Abstract Small cell lung cancer (SCLC) is a highly aggressive type of cancer, characterized by rapid proliferation, early metastatic spread, clinical recurrence and high rate mortality. Using in vivo insertional mutagenesis screening conjunction with cross-species genomic transcriptomic validation, we identified strong consistent signal for neuronal, synaptic, glutamatergic signaling gene sets murine human SCLC. We show that SCLC cells have the ability to develop intimate contacts neuronal...
An adaptive stress response involves various mediators and circuits orchestrating a complex interplay of physiological, emotional, behavioral adjustments. We identified population corticotropin-releasing hormone (CRH) neurons in the lateral part interstitial nucleus anterior commissure (IPACL), subdivision extended amygdala, which exclusively innervate substantia nigra (SN). Specific stimulation this circuit elicits hyperactivation hypothalamic-pituitary-adrenal axis, locomotor activation,...
Negative bias is an essential characteristic of depressive episodes leading patients to attribute more negative valence environmental cues. This affects all levels information processing including emotional response, attention and memory, the development maintenance symptoms. In this context, pleasant stimuli become less attractive unpleasant ones aversive, yet related neural circuits underlying remain largely unknown. By studying a mice model for depression chronically receiving...
Abstract The insular cortex (IC) plays key roles in emotional and regulatory brain functions is affected across psychiatric diseases. However, the brain-wide connections of mouse IC have not been comprehensively mapped. Here we traced whole-brain inputs outputs its rostro-caudal extent. We employed cell-type specific monosynaptic rabies virus tracings to characterize afferent onto either excitatory or inhibitory neurons, adeno-associated viral label efferent axons. While connectivity between...
Abstract Anatomically incomplete spinal cord injuries can be followed by functional recovery mediated, in part, the formation of intraspinal detour circuits. Here, we show that adult mice recover tactile and proprioceptive function following a unilateral dorsal column lesion. We therefore investigated basis this focused on plasticity column-medial lemniscus pathway. ascending root ganglion (DRG) axons branch grey matter substantially increase number these collaterals injury. These sensory...
Abstract The lateral hypothalamus (LH) is critically involved in the regulation of homeostatic energy balance. Some neurons LH express receptors for leptin (LepRb), a hormone known to increase expenditure and decrease intake. However, neuroanatomical inputs LepRb-expressing remain unknown. We used rabies virus tracing technology map these inputs, but encountered non-specific tracing. To optimize this minor cell population (LepRb not ubiquitously expressed LH), we LepRb-Cre mice assessed how...
Summary The large SARS-CoV-2 spike (S) protein is the main target of current COVID-19 vaccine candidates but can induce non-neutralizing antibodies, which may cause vaccination-induced complications or enhancement disease. Besides, encoding a functional S in replication-competent virus vector vaccines result emergence viruses with altered expanded tropism. Here, we have developed safe single round rhabdovirus replicon platform for enhanced presentation receptor-binding domain (RBD)....
Lyssaviruses are neurotropic rhabdoviruses thought to be restricted mammalian hosts, and originate from bats. The identification of lyssavirus sequences amphibians reptiles by metatranscriptomics thus comes as a surprise challenges the origin lyssaviruses. novel proposed American tree frog (ATFLV) anole lizard (ALLV) reveal substantial phylogenetic distances each other bat lyssaviruses, with ATFLV being most distant. As virus isolation has not been successful yet, we have here studied...