A5003146712- Platelet Disorders and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Cardiovascular Conditions and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Immune Response and Inflammation
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Antifungal resistance and susceptibility
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Veterinary Pharmacology and Anesthesia
- Cardiovascular Effects of Exercise
- Atrial Fibrillation Management and Outcomes
- Veterinary Equine Medical Research
- Muscle and Compartmental Disorders
- Hemostasis and retained surgical items
- Blood groups and transfusion
- Genetic and Kidney Cyst Diseases
- Sepsis Diagnosis and Treatment
- Heterotopic Ossification and Related Conditions
- Neurological diseases and metabolism
- Hemophilia Treatment and Research
- Parasitic Diseases Research and Treatment
- Burn Injury Management and Outcomes
- Skin and Cellular Biology Research
North Carolina State University
2023-2024
University of California, Davis
2016-2024
The University of Melbourne
2023
Missouri College
2019-2020
University of Missouri
2019-2020
The University of Texas Health Science Center at Houston
2020
University of London
2016
Royal Veterinary College
2016
University of Guelph
2006-2013
Clopidogrel is commonly prescribed to cats with perceived increased risk of thromboembolic events, but little information exists regarding its antiplatelet effects.To determine effects clopidogrel on platelet responsiveness in or without the A31P mutation MYBPC3 gene. A secondary aim was characterize variability feline responses clopidogrel.Fourteen healthy from a Maine Coon/outbred mixed Domestic cat colony: 8 homozygous for MYPBC3 gene and 6 wild-type mutation.Ex vivo study. All received...
To expand the number of conditions and interventions explored for their associations with thrombosis in veterinary literature to provide basis prescribing recommendations.A population exposure comparison outcome format was used represent patient, exposure, comparison, outcome. Population Exposure Comparison Outcome questions were distributed worksheet authors who performed comprehensive searches, summarized evidence, created guideline recommendations that reviewed by domain chairs. The...
Introduction Cats with cardiomyopathy face an increased risk of arterial thromboembolism (ATE). Although clopidogrel is frequently utilized to mitigate this risk, feline responses therapy exhibit variability. This study evaluated 2 viscoelastic devices, thromboelastography (TEG) and Viscoelastic Coagulation Monitor (VCM), for monitoring in cats comparison light transmission aggregometry (LTA). Methods Twenty-eight healthy received 7 days. Blood was collected at baseline after treatment...
Abstract Recent increases in the prevalence and severity of wildfires some regions have resulted an increased frequency veterinary burn patients. Few studies exist regarding diagnostics management wounds patients current knowledge is extrapolated from human literature research models. Post-burn cardiac injury a common finding predictor mortality echocardiography important tool monitoring response to therapy predicting outcome. We describe notable findings cats naturally exposed California...
Canine neutrophils release neutrophil extracellular traps (NETs) in response to lipopolysaccharide but NETs from clinical septic dogs had not been identified. The primary aim is describe the methodology of identifying and quantifying cytology samples foci with sepsis using immunofluorescence microscopy. Cytology including endotracheal tracheal wash (ETW), bronchoalveolar lavage (BAL), abdominal pleural effusion collected 5 (3 septic, 2 non-septic) were fixed, permeabilized stained for...
Abstract Objective To assess platelet function via multiple electrode aggregometry (MEPA) in dogs with septic peritonitis and healthy dogs. The secondary aim was to determine if there is prognostic significance changes observed Design Prospective, observational cohort study conducted from January 2012 March 2014. Setting University teaching hospital. Animals Twenty 23 Interventions None. Measurements Main Results MEPA using arachidonic acid, adenosine diphosphate, collagen (COL) as agonists...
Abstract Background Cats with hypertrophic cardiomyopathy (HCM) are at risk of cardiogenic arterial thromboembolism (CATE). Neutrophil extracellular traps (NETs) may be a potential biomarker and therapeutic target for in cats. Hypothesis/Objectives Characterize NETs cats HCM or CATE. We hypothesized that circulating assessed the form cell‐free DNA (cfDNA) citrullinated histone H3 (citH3) increased CATE associated reported predisposing factors thrombus formation. Animals Eighty‐five including...
Abstract Background Dual antithrombotic treatment (DAT) with clopidogrel and rivaroxaban sometimes is prescribed to cats hypertrophic cardiomyopathy at risk of thromboembolism. To date, no studies have evaluated their combined effects on platelet function. Objectives/Hypothesis Evaluate the safety DAT in healthy compare, ex vivo, platelet‐dependent thrombin generation agonist‐induced activation aggregation treated clopidogrel, rivaroxaban, or DAT. We hypothesized that would safely modulate...
Two non-pedigreed male castrated cats had persistent cyanosis over a 3-year observation period. Clinical cardiopulmonary evaluations did not reveal abnormalities, but the blood remained dark after exposure to air. Erythrocytic methemoglobin concentrations were high (~40% of hemoglobin) and cytochrome b5 reductase (CYB5R) activities in erythrocytes low (≤15% control). One cat intolerant exertion, other developed anemia died due an unidentified comorbidity. Whole-genome sequencing revealed...
Abstract Clopidogrel is converted to its active metabolite by cytochrome P450 isoenzymes and irreversibly inhibits platelet activation antagonizing the adenosine-diphosphate (ADP) receptor. It frequently used in cats with hypertrophic cardiomyopathy (HCM) prevent thromboembolic complications. However, significant interpatient variability of response clopidogrel therapy has been suspected. In this study, we assessed impact single nucleotide polymorphisms (SNPs) within ADP receptor ( P2RY1,...
Objectives Feline arterial thromboembolism (ATE), an often devastating outcome, was recently shown to affect 11.3% of cats with hypertrophic cardiomyopathy over 10 years. Current American College Veterinary Internal Medicine guidelines recommend the use clopidogrel in at risk for ATE, addition a factor Xa inhibitor very high or post-ATE cases. To date, no studies have examined safety efficacy this combined antithrombotic therapy. This retrospective case series aimed assess frequency and type...
Abstract Objective —To determine the effects of indwelling nasogastric intubation on gastric emptying rate liquid in horses. Animals —6 healthy Procedures —Horses were assigned to treatment and control groups a prospective randomized crossover study with washout period at least 4 weeks between trials. Acetaminophen (20 mg/kg) diluted 1 L distilled water was administered via tube time points 0, 12, 30, 48, 72 hours evaluate liquid-phase rate. In horses, tubes removed after administration...
Acute kidney injury (AKI), an abrupt loss of renal function, is a commonly encountered emergency in small animal practice. This article, the first two on AKI, reviews pathophysiology and diagnosis condition dogs cats. The second to be published June issue In Practice , will cover treatment, recovery prognosis associated with AKI.
Functional Toll-like receptor 4 (TLR4) has been characterized in human and murine platelets indicating that play a role inflammation hemostasis during sepsis. It is unclear whether canine could express functional TLR4 by responding to its ligand, lipopolysaccharide (LPS). We sought determine if dogs LPS-induced platelet activation requires co-stimulation with ADP or thromboxane A2 (TxA2). Canine were unstimulated (resting) activated thrombin prior flow cytometric microscopic analyses for...
Abstract Background Glanzmann's thrombasthenia (GT) is a congenital platelet disorder affecting approximately 1:1 000 people globally and characterized by impaired aggregation clot retraction. Autosomal recessive, loss‐of‐function, variants in ITGA2B or ITGB3 of the αIIbβ3 receptor cause disease humans. A cat affected macrothrombocytopenia was presented to UC Davis VMTH. Hypothesis/Objectives Severe thrombopathia this has an underlying genetic etiology. Animals single patient, 2 age‐matched...
Abstract Background Pulmonary hypertension (PH) in dogs with myxomatous mitral valve disease (MMVD) is caused by increased pulmonary venous pressure. Thrombosis, vascular remodeling, and vasoconstriction mediated platelets could exacerbate PH. Hypothesis Dogs PH will exhibit a hypercoagulable state, characterized platelet activation, platelet‐leukocyte, platelet‐neutrophil aggregate formation. Animals Eleven (≥3.5 kg) diagnosed MMVD 10 lacking Methods Prospective cohort ex vivo study. All...
Cardiogenic arterial thromboembolism (CATE) is a life-threatening complication of hypertrophic cardiomyopathy (HCM) with high mortality rate. As the primary responders in hemostasis, platelets play crucial role progression CATE. Procoagulant are subpopulation activated that facilitate thrombin generation to strengthen thrombus structure. Since their discovery, procoagulant have gained considerable amount attention due potential thromboembolic complications. Uncovering key phenotypic markers...
An inherited neurologic syndrome in a family of mixed-breed Oriental cats has been characterized as forebrain commissural malformation, concurrent with ventriculomegaly and interhemispheric cysts. However, the genetic basis for this autosomal recessive is unknown. Forty-three were genotyped on Illumina Infinium Feline 63K iSelect DNA Array used analyses. Genome-wide association studies, including sib-transmission disequilibrium test case-control analysis, homozygosity mapping, identified...
The primary objective of this study was to evaluate a novel flow cytometry-based assay quantifying platelet phosphorylation vasodilator-stimulated phosphoprotein (P-VASP) in cats that received clopidogrel treatment. Eight healthy 18.75 mg PO q24h for 7 days. Prior and after treatment, blood collected ADP-induced light transmission aggregometry (LTA) P-VASP measurement by cytometry. Flow cytometry levels used derive reactivity index (PRI) before Based on LTA findings, response ADP...