A5010426134- Peroxisome Proliferator-Activated Receptors
- NF-κB Signaling Pathways
- Cancer, Lipids, and Metabolism
- Plant biochemistry and biosynthesis
- Immune Response and Inflammation
- Animal Genetics and Reproduction
- Metabolism and Genetic Disorders
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and biosynthesis
- T-cell and Retrovirus Studies
- Adipose Tissue and Metabolism
- Veterinary Medicine and Surgery
- Molecular Biology Techniques and Applications
- Liver Disease Diagnosis and Treatment
- Diet and metabolism studies
- Gut microbiota and health
- Human-Animal Interaction Studies
- Neuroscience and Neuropharmacology Research
- Cancer-related Molecular Pathways
- Probiotics and Fermented Foods
- Ubiquitin and proteasome pathways
- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- Drug Transport and Resistance Mechanisms
- Genetic and Kidney Cyst Diseases
Hill Top Research (United States)
2010-2024
Hill's Pet Nutrition (United States)
2007-2022
ID Genomics (United States)
2003-2008
RELX Group (United States)
2005
University of Maryland, Baltimore
2001
Huntsville Hospital
2001
Vanderbilt University Medical Center
1997-1998
Howard Hughes Medical Institute
1995-1997
Center for Rheumatology
1997
Vanderbilt University
1995-1996
The eukaryotic transcription factor NF-kappa B plays a central role in the induced expression of human immunodeficiency virus type 1 and many aspects genetic program mediating normal T-cell activation growth. nuclear activity is tightly regulated from cytoplasmic compartment by an inhibitory subunit called I kappa alpha. This inhibitor rapidly phosphorylated degraded response to diverse set B-inducing agents, including mitogens, proinflammatory cytokines, viral transactivators such as Tax...
The inhibitor protein I kappa B alpha controls the nuclear import of transcription factor NF-kappa B. inhibitory activity is regulated from cytoplasmic compartment by signal-induced proteolysis. Previous studies have shown that signal-dependent phosphorylation serine residues 32 and 36 targets to ubiquitin-proteasome pathway. Here we provide evidence lysine 21 22 serve as primary sites for ubiquitination alpha. Conservative Lys-->Arg substitutions at both Lys-21 Lys-22 produce...
Electroconvulsive therapy (ECT) remains the treatment of choice for drug-resistant patients with depressive disorders, yet mechanism its efficacy unknown. Gene transcription changes were measured in frontal cortex and hippocampus rats subjected to sham seizures or 1 10 electroconvulsive (ECS), a model ECT. Among 3500–4400 RNA sequences detected each sample, ECS increased by 1.5- 11-fold decreased at least 34% expression 120 unique genes. The produced more than three times number gene seen...
Members of the nuclear factor (NF)-κB/Rel family transcription factors are induced during thymic selection and in mature T lymphocytes after ligation cell antigen receptor (TCR). Despite these findings, disruption individual NF-κB/Rel genes has revealed no intrinsic defect development cells, perhaps reflecting functional redundancy. To circumvent this possibility, lineage was targeted to express a trans-dominant form IκBα that constitutively represses activity multiple proteins. Transgenic...
Activation of the nuclear hormone peroxisome proliferator-activated receptor gamma (PPARgamma) inhibits cell growth and promotes differentiation in a broad spectrum epithelial derived tumor lines. Here we utilized microarray technology to identify PPARgamma gene targets intestinal cells. For each gene, induction or repression was seen with two structurally distinct agonists, change expression could be blocked by co-treatment specific antagonist. A majority genes regulated independently...
In resting T lymphocytes, the transcription factor NF-kappaB is sequestered in cytoplasm via interactions with members of I kappa B family inhibitors, including IkappaBalpha and IkappaBbeta. During normal T-cell activation, rapidly phosphorylated, ubiquitinated, degraded by 26S proteasome, thus permitting release functional NF-kappaB. contrast to its transient pattern nuclear induction during an immune response, constitutively activated cells expressing Tax transforming protein human...
Alterations in intracellular oxidative status activate several signal transduction pathways resulting distinct patterns of gene expression. Treatment colorectal cancer cells with antioxidants can lead to apoptosis by induction p21 through a mechanism involving CCAAT/enhancer-binding protein beta (C/EBPbeta). Herein, we demonstrate that the antioxidant pyrrolidinedithiocarbamate activates cAMP-dependent kinase (PKA) cell line DKO-1. Activation PKA phosphorylates Ser299 within C/EBPbeta, which...
The domestic cat has offered enormous genomic potential in the veterinary description of over 250 hereditary disease models as well occurrence several deadly feline viruses (feline leukemia virus -- FeLV, coronavirus FECV, immunodeficiency - FIV) that are homologues to human scourges (cancer, SARS, and AIDS respectively). However, realize this bio-medical potential, a high density single nucleotide polymorphism (SNP) map is required order accomplish phenotype association discovery. To remedy...
Peroxisome proliferator-activated receptor γ (PPARγ) and transforming growth factor-β (TGF-β) are key regulators of epithelial cell biology. However, the molecular mechanisms by which either pathway induces inhibition differentiation incompletely understood. We have identified factor-simulated clone-22 (<i>TSC-22</i>) as a target gene both pathways in intestinal cells. TSC-22 is member family leucine zipper containing transcription factors with repressor activity. Although little known...
The cat (Felis silvestris catus) shows significant variation in pelage, morphological, and behavioral phenotypes amongst its over 40 domesticated breeds. majority of the breed specific phenotypic presentations originated through artificial selection, especially on desired novel characteristics that arose only a few hundred years ago. Variations coat texture color hair often delineate breeds domestic animals. Although genetic basis several feline colors lengths are characterized, less is...
Activation of the nuclear hormone receptor peroxisome proliferator-activated γ (PPARγ) inhibits cell growth and induces differentiation in both adipocyte epithelial lineages, although it is unclear whether this occurs through common or cell-type specific mechanisms. We have identified four human colon cancer lines that do no undergo inhibition induce markers after exposure to PPARγ agonists. Sequence analysis gene revealed all contain a previously unidentified point mutation ninth α-helix...
A nutrition-based approach was utilized to examine the effects of fish oil and a polyphenol blend (with or without tomato pomace) on fecal microbiota plasma/fecal metabolomes. Forty dogs, aged 5-14 years, were fed washout food, then randomized consume control (fish test with crossed over food; each for 30 days. Several metabolites differed when comparing consumption either foods, but few changed significantly between foods. Plasma levels 4-ethylphenyl sulfate (4-EPS), metabolite associated...
Selective COX-2 inhibitors reduce adenoma formation and cancer progression in rodent models of colorectal cancer. To assess the therapeutic activity selective inhibitors, we tested effect SC-58125 treatment on growth human colon carcinoma cells nude mice. Delaying by 2, 4, or 7 weeks following implantation resulted a significant inhibition tumor growth. Furthermore, short-term (48 hours) with was sufficient to attenuate for up 15 days. did not alter rate at which underwent apoptosis, but...
Extracts of fresh mammalian brain contained substances which inhibited the “slow” closer and opener systems crayfish claw. Glutamic acid was one substances. Aspartic γ-aminobutyric were also active. had an inhibitory effect aspartic a stimulatory on heart.
Abstract The first objective was to describe and evaluate the relationship between ability of oxytocin stimulate activity phospholipase (PL) C its release prostaglandin (PG) F 2α in ovine endometrial tissue. Caruncular tissue collected from ovariectomized ewes after completion an 11-day steroid replacement protocol. In experiment 1, explants were incubated either presence (10 −6 m ) or absence for 0, 3, 10, 30 100 min examine time-course activation PLC PGF response oxytocin. An increase...