A5088677086- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
Karolinska Institutet
2017-2024
Karolinska University Hospital
2017
Adoptive transfer of natural killer (NK) cells can induce remission in patients with relapsed/refractory leukemia and myeloma. However, to date, clinical efficacy NK cell immunotherapy has been limited a sub-fraction patients. Here we show that steps incorporated the ex vivo manipulation/production products used for adoptive infusion, such as over-night IL-2 activation or cryopreservation followed by expansion, drastically decreases surface expression bone marrow (BM) homing chemokine...
Regulatory T cells (Treg) suppress anti-tumor immune responses and their infiltration in the tumor microenvironment is associated with inferior prognosis cancer patients. Thus, order to enhance responses, selective depletion of Treg highly desired. We found that treatment zoledronic acid (ZA) resulted a decrease frequency was significant increase proliferation natural killer (NK) peripheral blood patients metastatic cancer. In vitro, genome-wide transcriptomic analysis revealed alterations...
Abstract NK cell responsiveness to target cells is tuned by interactions between inhibitory receptors and their cognate HLA class I ligands in a process termed “NK education.” Previous studies addressing the role for education Ab-dependent cellular cytotoxicity (ADCC) show ambiguous results do not encompass full educational resolution. In this study, we systematically characterized human CD16-triggered degranulation toward defined tumor lines presence of either mAb rituximab or recently...
Abstract CAR-NK cells can induce remission in lymphoma patients. We speculate that the full potential of adoptive NK cell immunotherapy against is restricted by their poor lymph node (LN) homing capacity. Here, we have utilized a clinically approved transfection method with aim redirecting to LNs. Electroporation ex vivo expanded mRNAs coding for CCR7, CXCR5, and CD62L resulted increased vitro migration towards chemokines mouse LN-derived supernatant. Following infusion into SCID/Beige mice,...
KIR and NKG2A receptors educate human NK cells to stay responsive with diminished HLA class I. Here, we addressed whether the I-binding receptor LIR-1 (LILRB1/ILT2/CD85j), which is widely expressed on cells, can mediate education contribute antitumor functions of cells.Healthy donor either unstimulated, overnight cytokine-activated or ex vivo-expanded were used target cell lines. Phenotype function analysed using flow cytometry 51Cr-release assays.We found that inhibitory under specific...