A5066748559- Cancer Immunotherapy and Biomarkers
- Lung Cancer Treatments and Mutations
- Cell death mechanisms and regulation
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- FOXO transcription factor regulation
- Ferroptosis and cancer prognosis
- RNA and protein synthesis mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related molecular mechanisms research
- Melanoma and MAPK Pathways
- Nuclear Structure and Function
- RNA Research and Splicing
- Gastroesophageal reflux and treatments
- Cytokine Signaling Pathways and Interactions
- Lung Cancer Research Studies
- Inflammatory mediators and NSAID effects
- RNA modifications and cancer
- Protein Kinase Regulation and GTPase Signaling
- Economic and Financial Impacts of Cancer
- Transcranial Magnetic Stimulation Studies
- Helicobacter pylori-related gastroenterology studies
- Cancer Mechanisms and Therapy
- Nitric Oxide and Endothelin Effects
- Migraine and Headache Studies
Spanish National Cancer Research Centre
2005-2022
Centro de Investigación del Cáncer
2019-2022
Research Institute Hospital 12 de Octubre
2021-2022
Centre for Genomic Regulation
2017-2022
Cancer Research Center
2021
Cancer Clinic
2021
Centro de Investigación Biomédica en Red de Cáncer
2019
Universitat Pompeu Fabra
2017
Barcelona Institute for Science and Technology
2017
Oncode Institute
2011
FOXO proteins are direct targets of PI3K/Akt signaling and they integrate the signals several other transduction pathways at transcriptional level. transcription factors involved in normal cell homeostasis neoplasia, regulated by multiple post-transcriptional modifications. In cancer research, regulation is receiving increasing attention as their activation has been linked to cell-cycle arrest apoptosis. Hence, have proposed act tumor suppressors. Here, we applied a chemical biology approach...
Housekeeping genes of animal genomes cluster in the same chromosomal regions. It has long been suggested that this organization contributes to their steady expression across all tissues organism. Here, we show activity Drosophila housekeeping gene promoters depends on neighbors. By measuring ∼85,000 reporters integrated Kc167 cells, identified best predictors as contacts with and terminators active genes. Surprisingly, chromatin composition at insertion site enhancers were less informative....
Cancer originates from cells that have acquired mutations in genes critical for controlling cell proliferation, survival and differentiation. Often, tumors continue to depend on these so-called driver mutations, providing the rationale targeted anticancer therapies. To date, large-scale sequencing analyses revealed hundreds of human tumors. However, without their functional validation it remains unclear which correspond driver, or rather bystander, and, therefore, whether mutated gene...
Atherosclerosis is the main medical problem in Hutchinson-Gilford progeria syndrome, a rare premature aging disorder caused by mutant lamin-A protein progerin. Recently, we found that limiting progerin expression to vascular smooth muscle cells (VSMCs) sufficient hasten atherosclerosis and death
Abstract Background Independent luciferase reporter assays and fluorescent translocation have been successfully used in drug discovery for several molecular targets. We developed U2transLUC, an assay system which read-outs can be multiplexed to provide a powerful cell-based high content screening method. Results The U2transLUC is based on stable cell line expressing GFP-tagged FOXO transcription factor gene under the control of human FOXO-responsive enhancers. measures nuclear-cytoplasmic...
Tumor mutational burden (TMB) is a recently proposed predictive biomarker for immunotherapy in solid tumors, including non-small cell lung cancer (NSCLC). Available assays TMB determination differ horizontal coverage, gene content and algorithms, leading to discrepancies results, impacting patient selection. A harmonization study of assessment with available cohort patients NSCLC urgently needed.We evaluated the obtained two marketed next generation sequencing panels: TruSight Oncology 500...
Intracellular localization is essential for the regulated activity of many signaling molecules associated with disease-relevant pathways. High content screening a powerful technology to monitor impact small or interfering RNAs on translocation proteins within intact cells. Several assays have been developed measure nucleocytoplasmic shuttling like nuclear factor κB, FoxO, activated T-cells involved in distinct networks. However, since all these bear leucine-rich export signal (NES),...
MAP17 is a non-glycosylated membrane-associated protein that has been shown to be over-expressed in human carcinomas, suggesting possible role of this tumorigenesis. However, very little known about the molecular mechanism mediating tumor promoting properties MAP17. To analyze effect on cell survival, we used Rat1 fibroblasts model where Myc over-expression promotes apoptosis low serum conditions. In present work, report protects Rat1a from Myc-induced through reactive oxygen species...
Abstract Phosphoinositide 3′‐kinases (PI3Ks) constitute a family of lipid kinases implicated in signal transduction through tyrosine kinase receptors and heterotrimeric G protein‐linked receptors. PI3Ks are heterodimers made up four different 110‐kDa catalytic subunits (p110α, p110β, p110γ, p110δ) smaller regulatory subunit. Despite clear implication survival signaling, the contribution individual PI3K isoforms has not been elucidated. To address this issue, we generated Rat1 fibroblasts...
Abstract Background Biases of DNA repair can shape the nucleotide landscape genomes at evolutionary timescales. The molecular mechanisms those biases are still poorly understood because it is difficult to isolate contributions from damage. Results Here, we develop a genome-wide assay whereby same lesion repaired in different genomic contexts. We insert thousands barcoded transposons carrying reporter mismatch genome mouse embryonic stem cells. Upon inducing double-strand break between tandem...
BackgroundInhibition of Akt signaling is considered one the most promising therapeutic strategies for many cancers. However, rational target-orientated approaches to cell based drug screens anti-cancer agents have historically been compromised by notorious absence suitable control cells.Methodology/Principal FindingsIn order address this fundamental problem, we developed BaFiso, a live-cell screening platform identify specific inhibitors pathway. BaFiso relies on co-culture isogenic lines...
Lung cancer is the leading cause of mortality worldwide, with non-small cell lung (NSCLC) being most prevalent histology. While immunotherapy checkpoint inhibitors has shown outstanding results in NSCLC, precise identification responders remains a major challenge. Most studies attempting to overcome this handicap have focused on adenocarcinomas or squamous carcinomas. Among NSCLC subtypes, molecular and immune characteristics large carcinoma (LCC), which represents 10% cases, are not well...
Abstract Lung cancer leads mortality, being Non-Small-Cell Cancer (NSCLC) the most prevalent subtype. Immunotherapy with checkpoint inhibitors has shown promising results in NSCLC, yet this benefit is restricted to a subset of patients. Robust predictive biomarkers response are lacking. We seek multiparametric biomarker signature. hypothesize that specific molecular alterations NSCLC tumor cells have deep impact on immune microenvironment. Describing relationship critical predict...
Abstract Lung cancer is the leading cause of cancer-related deaths worldwide. Tyrosine kinase inhibitors (TKIs) targeting epidermal growth factor receptor (EGFR) activating mutations have significantly improved patient´s treatment with these alterations. However, 15-20% EGFR-mutated patients do not respond to therapy, even harboring same than responders. Little known about mechanisms involved in this primary resistance. This work aims characterize molecular aberrations clones intrinsic...
Abstract Lung cancer has the second highest incidence and leads mortality worldwide, being Non-Small Cell Cancer (NSCLC) most prevalent subtype. Immunotherapy with checkpoint blockers shown outstanding benefits in a subset of NSCLC patients, which are not accurately identified due to lack robust biomarkers response. We hypothesize that specific molecular alterations tumor cells impact immune microenvironment. Defining this association could improve prediction response immunotherapy. This...