A5012690359- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- CAR-T cell therapy research
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Cancer Research and Treatments
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- Immune cells in cancer
- Cancer Immunotherapy and Biomarkers
- Virology and Viral Diseases
- Cancer Cells and Metastasis
- interferon and immune responses
- Extracellular vesicles in disease
- Occupational and environmental lung diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Sarcoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Melanoma and MAPK Pathways
- Immune responses and vaccinations
Inserm
2013-2024
Nantes Université
2013-2024
Centre National de la Recherche Scientifique
2013-2024
Université d'Angers
2017-2024
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2008-2024
Mayo Clinic
2013-2017
University of Minnesota Rochester
2014
WinnMed
2014
Institut Pasteur
2013
Plasmacytoid dendritic cells (pDC) are antigen-presenting specialized in antiviral response. The measles virus vaccine is proposed as an antitumor agent to target and specifically kill tumor without infecting healthy cells.Here, we investigated, vitro, the effects of vaccine-infected on phenotype functions human pDC. We studied maturation antigen cross-presentation by pDC, exposed either alone, or UV-irradiated cells.We found that only induced pDC with a strong production IFN-α, whereas were...
Abstract Despite conventional medical and surgical treatments, malignant pleural mesothelioma (MPM) remains incurable. Oncovirotherapy (i.e., the use of replication-competent virus for cancer treatment) is currently explored in clinical trials. In this study, we investigated antineoplastic potential a new oncolytic viral agent, live-attenuated measles (MV) strain derived from Edmonston vaccine lineage (Schwarz strain). We evaluated both activity immunoadjuvant properties MV on tumor cells....
Background Malignant pleural mesothelioma (MPM) is a rare and aggressive cancer related to asbestos exposure. The tumor microenvironment content, particularly the presence of macrophages, was described as crucial for development disease. This work aimed at studying involvement M-CSF (CSF-1)/IL-34/CSF-1R pathway in formation macrophages MPM, using samples from patients. Methods Pleural effusions (PEs), frozen tumors, primary MPM cells cell lines used this study belong biocollections...
Abstract Background Lung cancers represent the main cause of cancer related-death worldwide. Recently, immunotherapy alone or in combination with chemotherapy has deeply impacted therapeutic care leading to an improved overall survival. However, relapse will finally occur, no efficient second line treatment so far. New therapies development based on comprehension resistance mechanisms is necessary. difficulties obtain tumor samples before and after first hamper clearly understand consequence...
Extracellular vesicles (EVs) are mediators of intercellular communication in the tumor microenvironment. Tumor EVs commonly associated with metastasis, immunosuppression or drug resistance. Viral infections usually increase EV secretion, but little is known about effect oncolytic viruses (OVs) on EVs. Here, we investigated impact vesicular stomatitis virus (VSV) and vaccinia secreted by human melanoma thoracic cancer cells. We found that OV infection increases production These contain...
Lung and colorectal cancers are responsible for approximately 2 million deaths each year worldwide. Despite continual improvements, clinical management of these diseases remains challenging development novel therapies with increased efficacy is critical to address major public health issues. Oncolytic viruses have shown promising results against that resistant conventional anticancer therapies. Vaccine strains measles virus (MV) exhibit such natural antitumor properties by preferentially...
// Carole Achard 1,2,3 , Nicolas Boisgerault Tiphaine Delaunay David Roulois Steven Nedellec 3,4 Pierre-Joseph Royer 3,5 Mallory Pain Chantal Combredet 6 Mariana Mesel-Lemoine Laurent Cellerin 7 Antoine Magnan 3,5,8 Frédéric Tangy Marc Grégoire and Jean-François Fonteneau 1 INSERM, UMR892, Institut de Recherche en Santé l'Université Nantes, France 2 CNRS, UMR6299, 3 Université 4 INSERM UMS016, SFR Santé, 5 UMRS1087, du Thorax, CNRS UMR3569, Unité Génomique Virale et Vaccination, Pasteur,...
Attenuated measles virus (MV) is currently being evaluated in clinical trials as an oncolytic therapeutic agent. Originally used for its lytic activity against tumor cells, it now admitted that the effectiveness of MV also lies ability to initiate antitumor immune responses through activation dendritic cells (DCs). In this study, we investigated capacity convert human blood myeloid CD1c+ DCs and plasmacytoid (pDCs) into cytotoxic effectors. We found induces expression protein TNF-related...
Aggressive regrowth of recurrent tumors following treatment-induced dormancy represents a major clinical challenge for treatment malignant disease. We reported previously that prostate tumors, which underwent complete macroscopic regression followed by aggressive regrowth, could be cured with vesicular stomatitis virus (VSV)–expressed cDNA library derived from tumor cells. By screening the protective, recurrence-derived VSV-cDNA library, here we identify topoisomerase-IIα (TOPO-IIα) as...
Attenuated measles virus (MV) exerts its oncolytic activity in malignant pleural mesothelioma (MPM) cells that lack type-I interferon (IFN-I) production or responsiveness. However, other the tumor microenvironment (TME), such as myeloid cells, possess functional antiviral pathways. In this study, we aimed to characterize interplay between MV and human MPM. We cocultured MPM cell lines with monocytes macrophages infected them MV. analyzed transcriptome of each type studied their secretion...
Oncolytic immunotherapy using oncolytic viruses (OV) has been shown to stimulate the antitumor immune response by inducing release of tumor-associated antigens (TAA) and danger signals from dying infected tumor cells. In this study, we sought determine if lysis cells induced different OV: measles virus, vaccinia vesicular stomatitis herpes simplex type I adenovirus or enterovirus, consequences on capacity present TAA, such as NY-ESO-1. We show that co-culture NY-ESO-1neg/HLA-DP4pos melanoma...
Understanding how incompletely cleared primary tumors transition from minimal residual disease (MRD) into treatment-resistant, immune-invisible recurrences has major clinical significance. We show here that this is mediated through the subversion of two key elements innate immunosurveillance. In first, role TNFα changes an antitumor effector against a growth promoter for MRD. Second, whereas induced natural killer (NK)-mediated cytokine response characterized by low IL6 and elevated IFNγ, PD-L1
We used a VSV-cDNA library to treat recurrent melanoma, identifying immunogenic antigens, allowing us target recurrences with immunotherapy or chemotherapy. Primary B16 melanoma tumors were induced regress by frontline therapy. Mice treated immunotherapy. A Th17 recall response was screen the for individual viruses encoding rejection subsequently targeted using Recurrent effectively cDNA from tumors. Recurrence-associated antigens identified included Topoisomerase-IIα, YB-1, cdc7 kinase, and...
// Joëlle S. Nader 1 , Jérôme Abadie 1, 2 Sophie Deshayes Alice Boissard 3, 4 Stéphanie Blandin 5 Christophe Blanquart Nicolas Boisgerault Olivier Coqueret Catherine Guette Marc Grégoire and Daniel L. Pouliquen CRCINA, INSERM, Université d'Angers, de Nantes, France ONIRIS, 3 Angers, ICO, Plate-Forme MicroPICell, SFR François Bonamy, Correspondence to: Pouliquen, email: daniel.pouliquen@inserm.fr Keywords: stroma; invasiveness; sarcomatoid mesothelioma; immune cells; rat model Received:...
Malignant pleural mesothelioma (MPM) is a cancer of the pleura that lacks efficient treatment. Oncolytic immunotherapy using oncolytic vaccinia virus (VV) may represent an alternative therapeutic approach for treatment this malignancy. Here, we studied activity VV thymidine kinase (TK)-ribonucleotide reductase (RR)-/green fluorescent protein (GFP) against MPM. This from Copenhagen strain deleted two genes encoding TK (J2R) and RR (I4L) express GFP. First, show in vitro VVTK-RR-/GFP...
Dendritic cells (DCs) are professional antigen-presenting involved in the control and initiation of immune responses.In vivo, DCs exposed at periphery to maturation stimuli migrate lymph nodes, where they receive secondary signals from CD4+ T helper cells.These become able initiate CD8+ cytotoxic lymphocyte (CTL) responses.However, vitro investigations concerning human monocyte-derived have never focused on their functional properties after such sequential maturation.Here, we studied DC...
Abstract Tumor endothelial (TECs) cells play a critical role in regulating immune responses within the tumor microenvironment (TME). However, mechanisms by which TECs modulate cell population remain unclear, particularly non-small lung cancer (NSCLC). Here, we investigated how NSCLC tweak normal (NECs) into and subsequent effects on regulation. NECs were cocultured with various lines, using 2D 3D coculture models to evaluate TEC-mediated cells. We show that direct led significant...