Abstract Phosphorus (P) is a macronutrient necessary for plant growth and development. Inorganic phosphate (Pi) deficiency modulates the signaling pathway of phytohormone jasmonate in Arabidopsis thaliana, but underlying molecular mechanism currently remains elusive. Here, we confirmed that was enhanced under low Pi conditions, CORONATINE INSENSITIVE1 (COI1)-mediated critical this process. A mechanistic investigation revealed several JASMONATE ZIM-DOMAIN (JAZ) repressors physically...

Physic nut (Jatropha curcas L.) is a potential feedstock for biofuel production because Jatropha oil highly suitable the of biodiesel and bio-jet fuels. However, exhibits low seed yield as result unreliable poor flowering. FLOWERING LOCUS T (FT) –like genes are important flowering regulators in higher plants. To date, have not yet been identified or characterized. better understand genetic control Jatropha, an FT homolog was isolated from designated JcFT. Sequence analysis phylogenetic...

CONSTANS (CO) is a critical regulator of flowering that combines photoperiodic and circadian signals in Arabidopsis (Arabidopsis thaliana). CO expressed multiple tissues, including seedling roots young leaves. However, the roles underlying mechanisms modulating physiological processes outside remain obscure. Here, we show expression responds to salinity treatment. negatively mediated tolerance under long-day (LD) conditions. Seedlings from co-mutants were more tolerant stress, whereas...

Jatropha curcas is a promising renewable feedstock for biodiesel and bio-jet fuel production. To study gene expression in different tissues throughout development under stress conditions, we examined total of 11 typical candidate reference genes using real-time quantitative polymerase chain reaction (RT-qPCR) analysis, which widely used validating transcript levels studies. The stability these was assessed across 20 samples, including various at vegetative reproductive stages desiccation...

Most germplasms of the biofuel plant Jatropha curcas are monoecious. A gynoecious genotype J. was found, whose male flowers aborted at early stage inflorescence development. To investigate regulatory mechanism transition from monoecious to plants, a comparative transcriptome analysis between and inflorescences were performed. total 3,749 genes differentially expressed in two developmental stages identified. Among them, 32 involved floral development, 70 phytohormone biosynthesis signaling...

The seed oil of Jatropha curcas is considered a potential bioenergy source that could replace fossil fuels. However, the yield low and has yet to be improved. We previously reported exogenous cytokinin treatment increased Jatropha. Cytokinin levels are directly regulated by isopentenyl transferase (IPT), cytochrome P450 monooxygenase, family 735, subfamily A (CYP735A), oxidase/dehydrogenase (CKX). In this study, we cloned six IPT genes, one JcCYP735A gene, seven JcCKX genes. expression...

<title>Abstract</title> Gene transcription regulation is essential for eukaryotic development. Despite the considerable advances has been made in cis-regulatory elements, roles of silencers gene transcription, particularly plants, remain largely elusive. Here, we employed a method called repressive self-transcribing active regulatory region sequencing (REST-seq) to systematically identify across Arabidopsis genome, modified from STARR-seq enhancer identification. In all, 13,032 were...

Plukenetia volubilis L., a woody oilseed plant rich in α-linolenic acid, represents promising source of polyunsaturated fatty acids. However, the lack an efficient genetic transformation system has significantly hindered gene function research and molecular breeding P. volubilis. In this study, we developed highly Agrobacterium rhizogenes-mediated hairy root for via use gel combination with visually detectable RUBY reporter analysis roots. The results indicate that optimal method involves...

Abscisic acid (ABA) suppresses Arabidopsis (Arabidopsis thaliana) seed germination and post-germinative growth. Nitrate stimulates germination, but whether it directly regulates ABA signaling the associated underlying molecular mechanisms remain unknown. Here, we showed that nitrate alleviates repressive effects of on independently nitric oxide (NO) pathway. Moreover, attenuates activated by ABSCISIC ACID INSENSITIVE3 (ABI3) ABI5, two critical transcriptional regulators Mechanistic analyses...

Real-time quantitative PCR (RT-qPCR) is a reliable and widely used method for gene expression analysis. The accuracy of the determination target level by RT-qPCR demands use appropriate reference genes to normalize mRNA levels among different samples. However, suitable have not been identified in Sacha inchi (Plukenetia volubilis), promising oilseed crop known its polyunsaturated fatty acid (PUFA)-rich seeds. In this study, using RT-qPCR, twelve candidate were examined seedlings adult...

Abstract Recent research revealed that TERMINAL FLOWER 1 (TFL1 ) homologues are involved in the critical developmental process of floral initiation several plant species. In this study, functions three putative TFL1 ( JcTFL1a, JcTFL1b and JcTFL1c biofuel Jatropha curcas were analysed using transgenic approach. , but not JcTFL1a could complement function rescue early flowering determinate inflorescence phenotype tfl1-14 Arabidopsis mutant, thus suggesting may be . Transgenic overexpressing or...

Plukenetia volubilis, whose seeds contain a high content of polyunsaturated fatty acids, produces approximately 60 male flowers but only 1–2 female per inflorescence. Increasing the number is critical for yield improvement P. volubilis. In this study, we determined effect plant growth regulator 6-benzyladenine (BA) on floral sex determination in Exogenous application BA converted most inflorescences to flowers, and 8–20% induced further developed into fruits. Treatment with various...

Jatropha curcas seeds are an excellent biofuel feedstock, but seed yields of limited by its poor flowering and fruiting ability. Thus, identifying genes controlling is critical for genetic improvement yield. We isolated the JcLFY, a ortholog Arabidopsis thaliana LEAFY (LFY), identified JcLFY function overexpressing it in Jatropha. expressed inflorescence buds, flower carpels, with highest expression early developmental stage buds. overexpression induced flowering, solitary flowers, terminal...

Jatropha curcas is considered a potential biodiesel feedstock crop. Currently, the value of J. limited because its seed yield generally low. Transgenic modification promising approach to improve curcas. Although Agrobacterium-mediated genetic transformation has been pursued for several years, efficiency remains unsatisfying. Therefore, highly efficient and simple method should be developed. We examined optimized key factors that affect in this study. The results showed EHA105 strain was...

Jatropha curcas is considered to be one of the greatest potential biofuel plants. However, application J. limited by a lack high yielding varieties. Polyploids often generate useful germplasm for genetic improvement in many Here, tetraploid and octoploid were obtained treating shoot tips seedlings with colchicine. The ploidy was determined measuring nuclear DNA content flow cytometry counting chromosomes. Treatment 0.1% colchicine seven days most effective condition producing polyploid...

Diacylglycerol acyltransferase (DGAT) is the only enzyme that catalyzes acyl-CoA-dependent acylation of sn-1, 2-diacylglycerol (DAG) to form triacylglycerol (TAG). The two main types DGAT enzymes in woody perennial biofuel plant Jatropha curcas, JcDGAT1 and JcDGAT2, were previously characterized heterologous systems. In this study, we investigated functions JcDGAT2 J. curcas.JcDGAT1 found be predominantly expressed during late stages curcas seed development, which large amounts oil...

Plukenetia volubilis is a promising oilseed crop due to its seeds being rich in unsaturated fatty acids, especially alpha-linolenic acid. P. monoecious, with separate male and female flowers on the same inflorescence. We previously reported that were converted by exogenous cytokinin (6-benzyladenine, 6-BA) treatment volubilis. To identify candidate genes associated floral sex differentiation of volubilis, we performed de novo transcriptome assembly comparative analysis control inflorescence...

Jatropha curcas L. is monoecious with a low female-to-male ratio, which one of the factors restricting its seed yield. Because phytohormone cytokinins play an essential role in flower development, particularly pistil this study, we elevated cytokinin levels J. flowers through transgenic expression biosynthetic gene (AtIPT4) from Arabidopsis under control orthologue TOMATO MADS BOX GENE 6 (JcTM6) promoter that predominantly active flowers. As expected, six species inflorescences were...

摘要: WRKY 转录因子家族在调控植物逆境诱导反应、生长发育及其信号转导等方面起着重要的分子生物学功能。文章采用Northern 杂交的方法, 对拟南芥3个WRKY基因进行表达谱分析。结果表明: AtWRKY25、AtWRKY26和AtWRKY33受多种非生物逆境因子(温度因子、高盐、渗透胁迫和激素脱落酸)的影响, 其中低温和高盐对AtWRKY25、AtWRKY26和AtWRKY33的诱导尤为明显, 表明这3个AtWRKY基因可能在响应环境信号方面起着一定的作用。作为序列相似性较高的AtWRKY25、AtWRKY26和AtWRKY33对一些胁迫因子的表达模式呈现一定的相似性; 但AtWRKY33受高温的抑制和低温的快速诱导, 与另外两个基因的表达模式不同, 推测它们对温度胁迫因子的反应存在差异。此外, 对启动子序列的生物信息学分析发现, 3个基因的启动子包含多个与非生物逆境反应相关的顺式作用元件。