Abstract The INDUCER OF CBF EXPRESSION (ICE)–C-REPEAT BINDING FACTOR/DRE FACTOR1 (CBF/DREB1) transcriptional pathway plays a critical role in modulating cold stress responses Arabidopsis thaliana. Dissecting crucial upstream regulatory signals or components of the ICE-CBF/DREB1 cascade will enhance our understanding plant cold-tolerance mechanisms. Here, we show that jasmonate positively regulates to freezing Arabidopsis. Exogenous application significantly enhanced tolerance with without...

Abstract Seed germination and postgerminative growth are regulated by a delicate hormonal balance. Abscisic acid (ABA) represses Arabidopsis thaliana seed growth, while brassinosteroids (BRs) antagonize ABA-mediated inhibition promote these processes. However, the molecular mechanism underlying BR-repressed ABA signaling remains largely unknown. Here, we show that Glycogen Synthase Kinase 3-like kinase BRASSINOSTEROID INSENSITIVE2 (BIN2), critical repressor of BR signaling, positively...

Abstract Gibberellin (GA) and photoperiod pathways have recently been demonstrated to collaboratively modulate flowering under long days (LDs). However, the molecular mechanisms underlying this collaboration remain largely unclear. In study, we found that GA-induced expression of FLOWERING LOCUS T (FT) LDs was dependent on CONSTANS (CO), a critical transcription factor positively involved in signaling. Mechanistic investigation revealed DELLA proteins, group crucial repressors GA signaling,...

ABSCISIC ACID INSENSITIVE5 (ABI5) is a crucial regulator of abscisic acid (ABA) signaling pathways involved in repressing seed germination and postgerminative growth Arabidopsis (Arabidopsis thaliana). ABI5 precisely modulated at the posttranslational level; however, transcriptional regulatory mechanisms underlying its interacting transcription factors remain largely unknown. Here, we found that INDUCER OF CBF EXPRESSION1 (ICE1) physically associates with ABI5. ICE1 negatively regulates ABA...

Abscisic acid (ABA) is known to suppress seed germination and post-germinative growth of Arabidopsis (Arabidopsis thaliana), jasmonate (JA) enhances ABA function. However, the molecular mechanism underlying crosstalk between JA signaling pathways remains largely elusive. Here, we show that exogenous coronatine, a analog structurally similar active conjugate jasmonate-isoleucine, significantly delayed response ABA. Disruption receptor CORONATINE INSENSITIVE1 or accumulation repressor...

Root hairs arise from trichoblasts and are crucial for plant anchorage, nutrient acquisition, environmental interactions. The phytohormone jasmonate is known to regulate root hair development in Arabidopsis (Arabidopsis thaliana), but little about the molecular mechanism underlying modulation this process. Here, we show that application of exogenous significantly stimulated elongation, but, on contrary, blocking perception or signaling resulted defective hairs. Jasmonate consistently...

Abscisic acid (ABA) represses seed germination and postgerminative growth in Arabidopsis thaliana. Auxin jasmonic (JA) stimulate ABA function; however, the possible synergistic effects of auxin JA on signaling underlying molecular mechanisms remain elusive. Here, we show that exogenous works synergistically with to enhance ABA-induced delay germination. biosynthesis, perception, are crucial for JA-promoted responses. The auxin-dependent transcription factors AUXIN RESPONSE FACTOR10 (ARF10)...

Abstract Seed germination and postgerminative growth require the precise coordination of multiple intrinsic environmental signals. The phytohormone abscisic acid (ABA) suppresses these processes in Arabidopsis thaliana circadian clock contributes to regulation ABA signaling. However, molecular mechanism underlying clock-mediated signaling remains largely unknown. Here, we found that core proteins PSEUDO-RESPONSE REGULATOR5 (PRR5) PRR7 physically associate with ABSCISIC ACID-INSENSITIVE5...

Abstract Cold stress affects plant immune responses, and this process may involve the salicylic acid (SA) signaling pathway. However, underlying mechanism by which low-temperature signals coordinate with SA to regulate immunity remains unclear. Here, we found that low temperatures enhanced disease resistance of Arabidopsis thaliana against Pseudomonas syringae pv. tomato DC3000. This required INDUCER OF CBF EXPRESSION 1 (ICE1), core transcription factor in cold-signal cascades. ICE1...

Seed germination and early seedling establishment, critical developmental stages in the life cycle of seed plants, are modulated by diverse endogenous hormones surrounding environment. Arabidopsis ABSCISIC ACID-INSENSITIVE5 (ABI5) is a central transcription factor abscisic acid (ABA) signaling that represses those processes. ABI5 precisely at post-translational level; however, whether it interacts with other crucial transcriptional regulators remains to be investigated. In this study, VQ18...

Arabidopsis VQ motif-containing proteins have recently been demonstrated to interact with several WRKY transcription factors; however, their specific biological functions and the molecular mechanisms underlying involvement in defense responses remain largely unclear. Here, we showed that two genes, VQ12 VQ29, were highly responsive necrotrophic fungal pathogen Botrytis cinerea. To characterize roles plant defense, generated amiR-vq12 transgenic plants by using an artificial miRNA approach...

CONSTANS (CO) is a critical regulator of flowering that combines photoperiodic and circadian signals in Arabidopsis (Arabidopsis thaliana). CO expressed multiple tissues, including seedling roots young leaves. However, the roles underlying mechanisms modulating physiological processes outside remain obscure. Here, we show expression responds to salinity treatment. negatively mediated tolerance under long-day (LD) conditions. Seedlings from co-mutants were more tolerant stress, whereas...

Stomata are ports that facilitate gas and water vapor exchange between plants their environment. Stomatal development is strictly regulated by endogenous signals environmental cues. Jasmonate an important signal modulates multiple physiological processes in plants, yet the molecular mechanisms underlying its interactions with other developmental signaling pathways remain poorly understood. Here, we show jasmonate negatively regulates stomatal Arabidopsis (Arabidopsis thaliana) cotyledons....

Summary Cellulose is a by‐product of agricultural production and an abundant waste. As carbon source, cellulose can be degraded utilized by fungi. Carbon sources, which act as nutrients, not only provide energy but also serve regulators gene expression, metabolism growth, through various signalling networks that enable cells to sense adapt varying environmental conditions. Nutrient‐sensing pathways prioritize the use preferred sources regulate cellulose‐degrading enzymes when necessary....

Pleurotus ostreatus (Jacq.) P. Kumm is the second most cultivated edible mushroom worldwide. Temperature one of main environmental factors affecting growth and fructification mushrooms. However, effects ambient temperature on growth, development, production bag-cultured are still unclear. In this study, eight temperatures (15 °C, 18 20 22 25 28 30 32 °C) were used to cultivate mycelia with cottonseed hull as substrate. Then, agronomic traits activities substrate-degrading antioxidant enzymes...

Abstract ABSCISIC ACID-INSENSITIVE3 (ABI3) and ABI5 are 2 crucial transcription factors in abscisic acid (ABA) signaling, their homeostasis at the protein level plays a decisive role seed germination subsequent seedling growth. Here, we found that PLANT U-BOX 8 (PUB8), U-box E3 ubiquitin ligase, physically interacts with ABI3 negatively regulates ABA responses during early Arabidopsis (Arabidopsis thaliana) Loss-of-function pub8 mutants were hypersensitive to ABA-inhibited cotyledon...

Abstract CONSTANS (CO) is a master flowering-time regulator that integrates photoperiodic and circadian signals in Arabidopsis thaliana. CO expressed multiple tissues, including young leaves seedling roots, but little known about the roles underlying mechanisms of mediating physiological responses other than flowering. Here, we show expression responsive to jasmonate. negatively modulated jasmonate-imposed root-growth inhibition anthocyanin accumulation. Seedlings from co mutants were more...

Anemone flaccida Fr. Shmidt (Ranunculaceae), commonly known as 'Di Wu' in China, is a perennial herb with limited distribution. The rhizome of A. has long been used to treat arthritis tradition China. Studies disclosed that the plant contains rich source triterpenoid saponins. However, little about saponins biosynthesis flaccida.In this study, we conducted tandem transcriptome and proteome profiling non-model medicinal plant, flaccida. Using Illumina HiSeq 2000 sequencing iTRAQ technique,...

Abstract Ganoderma lucidum is a legendary Traditional Chinese Medicine (TCM) over few thousands of years and one kind its major active components are Ganoderic acids (GAs). GAs largely produced in the mushroom primordium fruiting body but much less mycelium stage. However, little known on underlying regulatory mechanism. As saprophytic fungus, G. solely obtains nutrients by wood decaying. Wood general contains sophisticated chemical with diverse structural units. To explore strategy that...

Pleurotus ostreatus (Jacq.) P. Kumm is cultivated worldwide, and its growth seriously threatened by heat stress. Here, we performed a comprehensive analysis to investigate the influence of phytohormone salicylic acid (SA) in under HS. The results showed that hyphal recovery rate antioxidant capacity increased with exogenous SA application (0.01 mmol/L 0.05 mmol/L) after HS treatment. Metabolomic transcriptomic analyses (0.05 weakened central carbon metabolism allow cells survive efficiently....