A5100675496- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Circular RNAs in diseases
- Lipid metabolism and biosynthesis
- Chromosomal and Genetic Variations
- Plant Micronutrient Interactions and Effects
- Plant Diversity and Evolution
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Seed Germination and Physiology
- Infant Nutrition and Health
- African Botany and Ecology Studies
- MicroRNA in disease regulation
- Plant responses to water stress
- Transgenic Plants and Applications
- Molecular Biology Techniques and Applications
- Agricultural pest management studies
- Moringa oleifera research and applications
- Neonatal Respiratory Health Research
- Plant nutrient uptake and metabolism
- Biodiesel Production and Applications
- CRISPR and Genetic Engineering
Chinese Academy of Sciences
2015-2025
Xishuangbanna Tropical Botanical Garden
2015-2025
Guangzhou Medical University
2024
Second Affiliated Hospital of Guangzhou Medical University
2024
Agricultural Genomics Institute at Shenzhen
2023
Northwest A&F University
2023
Chinese Academy of Agricultural Sciences
2023
Ministry of Agriculture and Rural Affairs
2023
Real-time quantitative PCR (RT-qPCR) is a reliable and widely used method for gene expression analysis. The accuracy of the determination target level by RT-qPCR demands use appropriate reference genes to normalize mRNA levels among different samples. However, suitable have not been identified in Sacha inchi (Plukenetia volubilis), promising oilseed crop known its polyunsaturated fatty acid (PUFA)-rich seeds. In this study, using RT-qPCR, twelve candidate were examined seedlings adult...
Plukenetia volubilis, whose seeds contain a high content of polyunsaturated fatty acids, produces approximately 60 male flowers but only 1–2 female per inflorescence. Increasing the number is critical for yield improvement P. volubilis. In this study, we determined effect plant growth regulator 6-benzyladenine (BA) on floral sex determination in Exogenous application BA converted most inflorescences to flowers, and 8–20% induced further developed into fruits. Treatment with various...
Jatropha curcas is considered a potential biodiesel feedstock crop. Currently, the value of J. limited because its seed yield generally low. Transgenic modification promising approach to improve curcas. Although Agrobacterium-mediated genetic transformation has been pursued for several years, efficiency remains unsatisfying. Therefore, highly efficient and simple method should be developed. We examined optimized key factors that affect in this study. The results showed EHA105 strain was...
Jatropha curcas is considered to be one of the greatest potential biofuel plants. However, application J. limited by a lack high yielding varieties. Polyploids often generate useful germplasm for genetic improvement in many Here, tetraploid and octoploid were obtained treating shoot tips seedlings with colchicine. The ploidy was determined measuring nuclear DNA content flow cytometry counting chromosomes. Treatment 0.1% colchicine seven days most effective condition producing polyploid...
Diacylglycerol acyltransferase (DGAT) is the only enzyme that catalyzes acyl-CoA-dependent acylation of sn-1, 2-diacylglycerol (DAG) to form triacylglycerol (TAG). The two main types DGAT enzymes in woody perennial biofuel plant Jatropha curcas, JcDGAT1 and JcDGAT2, were previously characterized heterologous systems. In this study, we investigated functions JcDGAT2 J. curcas.JcDGAT1 found be predominantly expressed during late stages curcas seed development, which large amounts oil...
Plukenetia volubilis is a promising oilseed crop due to its seeds being rich in unsaturated fatty acids, especially alpha-linolenic acid. P. monoecious, with separate male and female flowers on the same inflorescence. We previously reported that were converted by exogenous cytokinin (6-benzyladenine, 6-BA) treatment volubilis. To identify candidate genes associated floral sex differentiation of volubilis, we performed de novo transcriptome assembly comparative analysis control inflorescence...
Jatropha curcas L. is monoecious with a low female-to-male ratio, which one of the factors restricting its seed yield. Because phytohormone cytokinins play an essential role in flower development, particularly pistil this study, we elevated cytokinin levels J. flowers through transgenic expression biosynthetic gene (AtIPT4) from Arabidopsis under control orthologue TOMATO MADS BOX GENE 6 (JcTM6) promoter that predominantly active flowers. As expected, six species inflorescences were...
Abstract Background Chromatin architecture is an essential factor regulating gene transcription in different cell types and developmental phases. However, studies on chromatin perennial woody plants the function of organization sex determination have not been reported. Results Here, we produced a chromosome-scale de novo genome assembly plant Jatropha curcas with total length 379.5 Mb scaffold N50 30.7 using Pacific Biosciences long reads combined genome-wide chromosome conformation capture...
In higher plants, inflorescence architecture is an important agronomic trait directly determining seed yield. However, little information available on the regulatory mechanism of development in perennial woody plants. Based two branching mutants, we investigated transcriptome differences buds between mutants and wild-type (WT) plants by RNA-Seq to identify genes networks controlling Jatropha curcas L., a plant belonging Euphorbiaceae.Two were identified germplasm collection Jatropha. The duo...
Purpose Circular RNAs (circRNAs) are products of alternative splicing with roles as competitive endogenous or microRNA sponges, regulating gene expression and biological processes. However, the involvement circRNAs in herpes simplex keratitis remains largely unexplored.
Abstract Background Plukenetia corniculata Sm., a member of the family Euphorbiaceae, is traditional leafy vegetable in Southeast Asia. Its young shoots, leaves, and fruits are consumed as vegetables, its mature seeds can be eaten nuts. Although P. not included list threatened species, habitat destruction has affected survivability some areas. To enhance conservation exploitation germplasm, more knowledge necessary to elucidate flowering characteristics reproductive biology. Results The...