A5048659357- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Insect Resistance and Genetics
- Cellular transport and secretion
- Insect and Pesticide Research
- Hemiptera Insect Studies
- Soybean genetics and cultivation
- Plant and Fungal Interactions Research
- Plant and animal studies
- Plant Molecular Biology Research
- Agricultural pest management studies
- Toxin Mechanisms and Immunotoxins
- Insect and Arachnid Ecology and Behavior
- Cocoa and Sweet Potato Agronomy
- Crop Yield and Soil Fertility
- Soil Management and Crop Yield
- Insect symbiosis and bacterial influences
- Lysosomal Storage Disorders Research
- Lipid Membrane Structure and Behavior
Ningbo University
2020-2025
Ministry of Agriculture and Rural Affairs
2025
Northeast Agricultural University
2024
China Jiliang University
2023
Institute of Crop Sciences
2019-2021
Chinese Academy of Agricultural Sciences
2019-2021
Zhejiang University
2020
Abstract Salicylic acid (SA) and jasmonic (JA) are plant hormones that typically act antagonistically in dicotyledonous plants SA JA signaling is often manipulated by pathogens. However, monocotyledonous plants, the detailed SA-JA interplay response to pathogen invasion remains elusive. Here, we show different types of viral can disrupt synergistic antiviral immunity mediated via OsNPR1 monocot rice. The P2 protein rice stripe virus, a negative-stranded RNA virus genus Tenuivirus , promotes...
Plant auxin response factor (ARF) transcription factors are an important class of key transcriptional modulators in signaling. Despite the well-studied roles ARF plant growth and development, it is largely unknown whether, how, may be involved resistance to pathogens. We show here that two fijiviruses (double-stranded RNA viruses) utilize their proteins disturb dimerization OsARF17 repress its activation ability, while a tenuivirus (negative-sense single-stranded virus) directly interferes...
Summary Soybean staygreen syndrome, characterized by delayed leaf and stem senescence, abnormal pods, aborted seeds, has recently become a serious prominent problem in soybean production. Although the pest Riptortus pedestris received increasing attention as possible cause of mechanism remains unknown. Here, we clarify that direct feeding R. , not transmission pathogen this pest, is primary typical syndrome critical damage occurs at early pod stage . Transcriptome profiling indicated many...
Abstract Herbivorous insects employ an array of salivary proteins to aid feeding. However, the mechanisms behind recruitment and evolution these genes mediate plant-insect interactions remain poorly understood. Here, we report a potential horizontal gene transfer (HGT) event from bacteria ancestral bug Eutrichophora. The acquired subsequently underwent duplications evolved through co-option. We annotated them as horizontal-transferred, Eutrichophora-specific protein (HESPs) according their...
Significance To accomplish the infection cycle, plant viruses usually employ various pathogenic factors to inhibit host antiviral defense. Revealing mechanisms conserved among different is essential for developing broad-spectrum disease control. Here, we show that a class of independently evolved transcriptional repressors widely present in viruses. Despite diverse sequences and evolutionary origins, these all modulate JA signaling by inhibiting activation OsMYC transcription factors,...
NF-Y transcription factors are known to play many diverse roles in the development and physiological responses of plants but little is about their role plant defense. Here, we demonstrate negative rice NF-YA family genes antiviral defense against two different viruses, Rice stripe virus (RSV, Tenuivirus) Southern black-streaked dwarf (SRBSDV, Fijivirus). RSV SRBSDV both induced expression OsNF-YA genes. Overexpression OsNF-YAs enhanced susceptibility infection, while RNAi mutants were more...
Plant viruses adopt diverse virulence strategies to inhibit host antiviral defense. However, general defense directly targeted by different types of plant have rarely been studied. Here, we show that the single rice DELLA protein, SLENDER RICE 1 (SLR1), a master negative regulator in Gibberellin (GA) signaling pathway, is several viral effectors for facilitating infection. Viral proteins encoded all trigger rapid degradation SLR1 promoting association with GA receptor OsGID1. SLR1-mediated...
Lysine acetylation is a dynamic post-translational modification of proteins. Extensive studies have revealed that the modulated by histone acetyltransferases and deacetylases (HDACs) plays crucial role in regulating protein function. However, there has been limited focus on how HDACs regulate jasmonic acid (JA) biosynthesis plants. Here, we uncover stability OsLOX14, critical enzyme involved JA biosynthesis, regulated deacetylase, OsHDA706, hindered viral protein. Our results show OsHDA706...
Abstract Plant viruses have multiple strategies to counter and evade the host’s antiviral immune response. However, limited research has been conducted on defense mechanisms commonly targeted by distinct types of plant viruses. In this study, we discovered that NUCLEAR FACTOR-YC (NF-YC) FACTOR-YA (NF-YA), 2 essential components NF-Y complex, were viral proteins encoded different rice (Oryza sativa L.) viruses, stripe virus (RSV, Tenuivirus) southern black streaked dwarf (SRBSDV, Fijivirus)....
Dense vesicles (DVs) are vesicular carriers, unique to plants, that mediate post-Golgi trafficking of storage proteins protein vacuoles (PSVs) in seeds. However, the molecular mechanisms regulating directional targeting DVs PSVs remain elusive. Here, we show rice (Oryza sativa) glutelin precursor accumulation5 (gpa5) mutant is defective PSVs, resulting discharge its cargo into extracellular space. Molecular cloning revealed GPA5 encodes a plant-unique phox-homology domain-containing...
Summary Plants sense pathogen attacks using a variety of receptors at the cell surface. The LRR receptor‐like proteins (RLP) and kinases (RLK) are widely reported to participate in plant defence against bacterial fungal invasion. However, role RLP RLK antiviral has rarely been reported. We employed high‐throughput‐sequencing approach, transgenic rice plants viral inoculation assays investigate OsRLP1 OsSOBIR1 immunity virus infection. transcript protein, OsRLP1, was markedly up‐regulated...
The stinkbug Riptortus pedestris , notorious for inducing soybean staygreen-like syndrome, employs a range of salivary proteins to manipulate the host plant its benefit. Here, we show that RpSP1, protein specific Pentatomomorpha, triggers defense responses in multiple species. RpSP1 interacts with and stabilizes HSP40 family GmSPIP1 is dependent on induce cell death. We critical 22-amino acid peptide within acts as an intracellular insect-derived elicitor. Furthermore, enhances...
Abstract A variety of plant viruses employ different virulence strategies to achieve successful infection, resulting in abnormal development. However, the common pathogenicity distinct has rarely been studied. Here, it is shown that a plant‐specific RAV‐type transcription factor, OsRAV15, specifically targeted by several viral proteins for facilitating infection. OsRAV15 found activate jasmonic acid (JA)‐mediated broad‐spectrum antiviral immunity physically associating with JA signaling...
Abstract The bean bug ( Riptortus pedestris ) causes great economic losses of soybeans by piercing and sucking pods seeds. Although R . has become the focus numerous studies associated with insect–microbe interactions, plant–insect pesticide resistance, a lack genomic resources limited deeper insights. Here, we report first genome at chromosomal level using PacBio, Illumina, Hi‐C technologies. assembled was 1.080 Gb in size contig N50 2.882 Mb. More than 96.3% total bases were successfully...
Protein storage vacuoles (PSVs) are unique organelles that accumulate proteins in plant seeds. Although morphological evidence points to the existence of multiple PSV-trafficking pathways for protein targeting, molecular mechanisms regulate these processes remain mostly unknown. Here, we report functional characterization rice (Oryza sativa) glutelin precursor accumulation7 (gpa7) mutant, which over-accumulates 57-kDa precursors dry Cytological and immunocytochemistry studies revealed gpa7...
The bean bug (Riptortus pedestris), one of the most important pests soybean, causes staygreen syndrome, delaying plant maturation and affecting pod development, resulting in severe crop yield loss. However, little is known about underlying mechanism this pest. In study, we found that a salivary secretory protein, Rp614, induced cell death nonhost Nicotiana benthamiana leaves. NbSGT1 NbNDR1 are involved Rp614-induced death. Tissue specificity analysis showed Rp614 mainly present glands highly...
The RAV family is part of the B3 superfamily and one most abundant transcription factor families in plants. Members have highly conserved or AP2 DNA binding domains. Although genes several species been systematically identified from genome-wide studies, there has no comprehensive study to identify rice genes. Here, we 15 genome analyzed their phylogenetic relationships, gene structure, domains, chromosomal distribution. Based on domain similarity topology, factors were phylogenetically...
Abstract Soybean mosaic virus (SMV; Potyvirus , Potyviridae ) is one of the most prevalent and destructive viral pathogens in world. The P1 protein first N-terminal product potyvirus genome shows a high sequence variability that may be related to adaptation hosts. In this work, we focused on different functions proteins two SMV isolates GZL NB during their infection plants. Isolate induced weaker symptoms than mechanical inoculation assays, accumulation level CP -infected leaves was lower...
The bean bug, Riptortus pedestris (Fabricius), is one of the most important soybean pests. It damages leaves and pods with its piercing-sucking mouthparts, causing staygreen-like syndromes in infested crops. During feeding process, R. secretes a mixture salivary proteins, which play critical roles insect–plant interactions may be responsible for syndromes. present study aimed to identify major proteins saliva by transcriptomic proteomic approaches, screen that potentially induced plant...
Abstract Riptortus pedestris (Fabricius), one of the major piercing‐sucking insects in soybeans, causes delayed plant senescence and abnormal pods, known as staygreen syndrome. Recent research has shown that direct feeding this insect is cause soybean However, it remains unclear whether R. salivary proteins play vital roles infestation. Here, we found 4 secretory can induce cell death Nicotiana benthamiana by transient heterologous expression. The induced Rp2155 relies on nucleotide‐binding...
Cowpea mild mottle virus (CPMMV) is a global plant that poses threat to the production and quality of legume crops. Early accurate diagnosis essential for effective managing CPMMV outbreaks. With advancement in isothermal recombinase polymerase amplification lateral flow strips technologies, more rapid sensitive methods have become available detecting this pathogen. In study, we developed reverse transcription combined with (RT-RPA-LFS) method detection CPMMV, specifically targeting coat...
Cowpea mild mottle virus (CPMMV; genus Carlavirus ) can be a destructive pathogen of soybean but there is little information about its distribution on in China. Here, we collected plants with virus-like symptoms from 11 fields widely scattered within China, and used high-throughput sequencing to determine their virome. Most samples (8/11) were co-infected by the well-studied potyvirus mosaic (SMV) CPMMV, remaining three singly infected CPMMV. The near-complete genome sequences CPMMV isolates...
Abstract Oligosaccharins, widely recognized as plant immunity inducers, have been applied and studied for enhancing antiviral defenses in rice over the years. However, due to complex induction mechanism rice, further studies are needed. In this study, we demonstrated that oligosaccharins enhanced resistance southern black-streaked dwarf virus (SRBSDV) at both symptomatic molecular levels. RNA-seq analysis revealed defense by activating multiple pathways. Among them, jasmonic acid (JA)...
Introduction Plant auxin response factors (ARFs) play an irreplaceable role in regulating the expression of genes. Our previous studies have indicated that factor OsARF17 plays a crucial plant defense against diverse rice viruses. Methods Utilizing comparative transcriptome analysis Rice stripe mosaic virus (RSMV)-inoculated mutant plants, to further elucidate molecular mechanism antiviral pathway. Results KEGG enrichment analyses showed down-regulated differentially expressed genes (DEGs)...
Storage protein is the most abundant nutritional component in soybean seed. Morphology-based evidence has verified that storage proteins are initially synthesized on endoplasmic reticulum, and then follow Golgi-mediated pathway to vacuole. However, molecular mechanisms of trafficking remain unknown. Here, we clone homologs Rab5 its guanine nucleotide exchange factor (GEF) VPS9. GEF activity combined with yeast two-hybrid assays demonstrated GmVPS9a2 might specifically act as canonical Rab5,...