Plant R2R3-MYB transcription factor genes are widely distributed in higher plants and play important roles the regulation of many secondary metabolites at transcriptional level. In this study, a chrysanthemum subgroup 4 gene, designated CmMYB1, was isolated through screening EST (expressed sequence tag) libraries using rapid application cDNA ends (RACE) methods functionally characterized. CmMYB1 is expressed root, stem, leaf flowers, but most strongly stem weakly root. Its heterologous...

Histone deacetylases (HDACs) play a critical role in the proliferation, differentiation, and apoptosis of cancer cells. An obstacle for application HDAC inhibitors as effective anti-cancer therapeutics is that our current knowledge on contributions different HDACs various types remains scarce. The present study reported mRNA protein levels HDAC5 were up-regulated human hepatocellular carcinoma (HCC) tissues cells shown by quantitative real-time PCR Western blot. MTT assay BrdU incorporation...

Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is an effective method for quantifying expression levels of target genes. The accuracy qRT-PCR results largely dependent on the selection stable reference stability gene may vary with plant species and environmental conditions. objective this study was to select genes analysis in different organs under abiotic stresses a perennial grass species, bermudagrass (Cynodon dactylon). eight potential (TUB, ACT, GAPDH,...

Abstract Osteoarthritis (OA) is a common chronic degenerative joint disease associated with variety of risk factors including aging, genetics, obesity, and mechanical disturbance. This study aimed to elucidate the function newly discovered circular RNA (circRNA), circFNDC3B, in OA progression its relationship NF-κB signaling pathway oxidative stress. The circFNDC3B/miR-525-5p/HO-1 axis stress were investigated validated using fluorescence situ hybridization, real-time PCR, western blotting,...

The family of NITRATE TRANSPORTER 2 (NRT2) proteins belongs to the high affinity transport system (HATS) which acts at low nitrate concentrations. relevant gene content chrysanthemum genome was explored here by isolating full length sequences six distinct CmNRT2 genes. One these (CmNRT2.1) investigated functional level. Its transcription level inducible concentrations both and ammonium. A yeast two hybrid assay showed that CmNRT2.1 interacts with CmNAR2, while a BiFC demonstrated interaction...

The fall armyworm, Spodoptera frugiperda (J. E. Smith), is one of the most important pests in tropical and subtropical regions American. S. was first detected Southern China January 2019, then subsequently invaded 26 provinces. Spinetoram widely used for pest management recommended control. sublethal effects spinetoram on were investigated present study. toxicity against larvae determined after oral dose at concentration. results showed that LC10 LC30 0.011 0.044 mg/liter larvae,...

BACKGROUND: Resistance to carbendazim and other benzimidazole fungicides in Botrytis cinerea (Pers. ex Fr.) most fungi is usually conferred by mutation(s) a single chromosomal β-tubulin gene, often with several allelic mutations. In Fusarium graminearum Schwade, however, resistance not associated mutation the corresponding gene. RESULTS: The gene conferring B. was cloned connected two homologous arms of F. using double-joint polymerase chain reaction (PCR). This fragment transferred into via...

Acute diarrhea leads to a substantial disease burden among the elderly worldwide. However, in context of increasingly aging trend China, prevalence etiological agents diarrheal patients was undetermined. This study aimed explore major enteropathogens acute outpatients older than 65 years and also epidemiological features pathogens. Demographic clinical data for were collected from 213 participating hospitals 2009 2014. Stool specimens tested 13 enteric viruses bacteria. The proportion...

To understand the molecular mechanisms of salinity tolerance during seed germination and post-germination stages, this study characterized phenotypic transcriptome responses two cotton cultivars stress. The were salt-tolerant (ST) LMY37 salt-sensitive (SS) ZM12, with former exhibiting higher rate, growth, primary-root fresh weight under Transcriptomic comparison revealed that up-regulation differentially expressed genes (DEGs) was main characteristic transcriptional regulation in ST, while...

Chrysanthemum is an important ornamental plant which increasingly being monocropped. Monocropping known to affect both fungal abundance and species diversity. Here, quantitative PCR allied with DGGE analysis was used show that fungi were more abundant in the rhizosphere than bulk soil populations changed during growth cycle of chrysanthemum. The majority amplified fragments appeared derive from Fusarium species, F. oxysporum solani proved be major pathogenic are built up by monocropping.

Objective. Previous studies have shown that lower serum amylase levels are associated with chronic pancreatitis. However, recently, been metabolic syndrome (MS) and diabetes. The objective of the present study was to investigate relationship between diabetes in Chinese asymptomatic individuals living Hangzhou, China. Methods. Serum amylase, triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL-c), low (LDL-c), γ-glutamyltransferase (γ-GT), sensitivity C reactive protein...

Bermudagrass (Cynodon dactylon) is a salinity-tolerant turfgrass that has good use potential in the saline-alkali lands of warm regions. However, systematic Na+ and K+ regulation mechanisms under salinity stress remain unclear at whole plant level. Two bermudagrass cultivars differing tolerance were exposed to 0, 50, 100, 200, or 300 mM NaCl hydroponic system. Growth, absorption, transportation, secretion K+, gas exchange parameters determined both cultivars. contents decreased Na+/K+ ratios...

Abstract Auxins are phytohormones that essential for many aspects of plant growth and development. The main auxin produced by plants is indole-3-acetic acid (IAA). IAA exists in free conjugated forms, corresponding to the bioactive stored hormones, respectively. Free levels, which crucial various physiological activities, maintained through a complex network environmentally developmentally responsive pathways including biosynthesis, transport, degradation, conjugation, conjugate hydrolysis....

Wild rice ( Oryza rufipogon L) is recognized as an important germplasm that has abundant genetic diversity and specific characters. Many of breeders attempt to utilize elite wild for improvement breeding program broaden background cultivating rice. Although There are some reports germplasms were successfully applied in program, it definitely depend on what we understand the traits particular complex traits. In this research goal was identify alleles yield related components. An advanced...

SummaryHybrid bermudagrass [Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy] is the type of turfgrass widely grown in tropical, sub-tropical, and warm temperate regions. Breeding salt-tolerant important for turf irrigated with reclaimed or saline water, established soils. Two experiments were conducted to evaluate variation salt-tolerance, shoot Na+, K+, Cl– ion concentrations, their inter-relationships 13 genotypes hybrid bermudagrass. Inter-genotypic salt-tolerance was based on...

Climate change is a major environmental concern and directly related to the increasing concentrations of greenhouse gases. The increase in atmospheric carbon dioxide (CO2), not only affects plant growth development, but also emission organic volatile compounds (VOCs). Changes odor profile may affect plant-insect interactions, especially behavior herbivorous insects. In this study, we compared foraging corn leaf aphid (Rhopalosiphum maidis) on barley (Hordeum vulgare L.) seedlings grown under...