A5074016992- Malaria Research and Control
- Parasitic Infections and Diagnostics
- Mosquito-borne diseases and control
- Amoebic Infections and Treatments
- vaccines and immunoinformatics approaches
- Privacy-Preserving Technologies in Data
- HIV Research and Treatment
- Complement system in diseases
- Cryptography and Data Security
- Pediatric health and respiratory diseases
- Anomaly Detection Techniques and Applications
- Petroleum Processing and Analysis
- BIM and Construction Integration
- Microbial bioremediation and biosurfactants
- Product Development and Customization
- Blockchain Technology Applications and Security
- Vector-borne infectious diseases
- COVID-19 epidemiological studies
- Computational Drug Discovery Methods
- Invertebrate Immune Response Mechanisms
- Manufacturing Process and Optimization
- Lipid Membrane Structure and Behavior
- Sustainable Industrial Ecology
- Optimization and Variational Analysis
- Design Education and Practice
Southeast University
2024-2025
Sinopec Research Institute of Petroleum Processing
2023
Sinopec (China)
2023
Xi'an Shiyou University
2023
Emory University
2008-2022
Guangxi Medical University
2022
Centers for Disease Control and Prevention
2003-2021
Energy Storage Systems (United States)
2021
Wuhan University
2020-2021
Xiangya Hospital Central South University
2020
ABSTRACT To assess the source and public health significance of Cryptosporidium oocyst contamination in storm runoff, a PCR-restriction fragment length polymorphism technique based on small-subunit rRNA gene was used analysis 94 water samples collected from Malcolm Brook N5 stream basins New York over 3-year period. The distribution this study compared with data obtained 27 Ashokan previous study. These three watersheds represented different levels human activity. Among total 121 analyzed...
ABSTRACT Extraction of high-quality DNA is a key step in PCR detection Cryptosporidium and other pathogens environmental samples. Currently, oocysts water samples have to be purified from concentrates before extracted. This study compared the effectiveness six extraction methods (DNA with QIAamp minikit after oocyst purification immunomagnetic separation direct using FastDNA SPIN kit for soil, stool minikit, UltraClean soil kit, or traditional phenol-chloroform technique) oocyst-seeded...
Building Information Modeling (BIM) is envisioned as an indispensable opportunity in the architecture, engineering, and construction (AEC) industries a revolutionary technology process. Smart relies on BIM for manipulating information flow, data management flow. Currently, model has been explored mainly utilization, but rare works pay efforts to security, e.g., critical audit sensitive exposure. Moreover, few systems are proposed chase after upcoming computing paradigms, such mobile cloud...
Collaborative learning allows multiple clients to train a joint model without sharing their data with each other. Each client performs training locally and then submits the updates central server for aggregation. Since has no visibility into process of generating updates, collaborative is vulnerable poisoning attacks where malicious can generate poisoned update introduce backdoor functionality model. The existing solutions detecting however, fail defend against recently proposed attacks,...
Federated learning facilitates the collaborative training of a global model among distributed clients without sharing their data. Secure aggregation, new security primitive for federated learning, aims to preserve confidentiality both local models and Unfortunately, existing secure aggregation solutions fail defend against Byzantine failures that are common in computing systems. In this work, we propose efficient framework, SEAR, Byzantine-robust learning. Relying on trusted execution...
ABSTRACT Six Cryptosporidium spp. were found in 50 of 179 Milwaukee wastewater samples collected weekly over a year. Of the eight subtypes hominis and parvum present, allele Ib was 14 16 samples, its sequence identical to that subtype human from 1993 outbreak cryptosporidiosis.
Traditional construction supply chains suffer from extra delays, costs and information wastages due to intermediaries. Blockchain, a decentralized infrastructure, can provide irreversibility, undeniableness, uniqueness anonymity for trades. Hence, we first propose blockchain-based chain framework reduce limitations in traditional ones. However, payment security by blockchain must be guaranteed token assets accounts protected. Although the loss of private keys will not result exposure or...
ABSTRACT Giardia cysts in 131 raw wastewater samples from Milwaukee, Wis., were genotyped by sequence analysis of the triosephosphate isomerase gene which showed presence two distinct genotypes (assemblages A and B) duodenalis . Of samples, 111 belonged to assemblage A, remaining B. high degree genetic polymorphism was evident within B cluster, with 10 subgenotypes identified, eight have not been reported before.
Summary Plasmodium vivax and P. cynomolgi produce numerous caveola–vesicle complex (CVC) structures within the surface of infected erythrocyte membrane. These contrast with electron‐dense knob protrusions expressed at falciparum ‐infected erythrocytes. Here we investigate three‐dimensional (3‐D) structure CVCs identity a predominantly 95 kDa CVC protein. Liquid chromatography – tandem mass spectrometry analysis immunoprecipitates by monoclonal antibodies from extracts identified this protein...
Background Plasmodium vivax is the most geographically widespread human malaria parasite. Cohort studies in Papua New Guinea have identified a rapid onset of immunity against vivax-malaria children living highly endemic areas. Although numerous P. merozoite antigens are targets naturally acquired antibodies, role many these antibodies protective yet unknown. Methodology/Principal Findings In cohort aged 1–3 years, to different regions Merozoite Surface Protein 3α (PvMSP3α) and 9 (PvMSP9)...
Background The antibody response generated during malaria infections is of particular interest, since the production specific IgG antibodies required for acquisition clinical immunity. However, variations in responses could result from genetic polymorphism HLA class II genes. Given increasing focus on development subunit vaccines, studies influence alleles immune ethnically diverse populations important, prior to implementation vaccine trials. Methods and Findings In this study, we evaluated...
Cryptosporidium parvum is an intracellular protozoan that can cause severe diarrhea in humans and various mammals. Results of a comparative genomic analysis indicated genes encoding two C. parvum-specific insulinase-like proteases (INS19 INS20), cgd6_5510 cgd6_5520, are lost many species. In this study, we provided evidence indicating cgd6_5520 fragments full gene (cgd6_5520-5510) one protease (INS20-19) similar structure to classic insulinases. We expressed Escherichia coli for antiserum...
ABSTRACT: The performance of 10 commonly used genotyping tools in the detection and differentiation 7 human‐pathogenic Cryptosporidium spp. ( C. hominis, parvum, meleagridis, felis, canis, muris pig genotype I) was evaluated. All 3 SU rRNA gene‐based could amplify DNA efficiently. However, based on antigens TRAP‐C1, TRAP‐C2 COWP genes, housekeeping genes HSP70 DHFR, or a genomic sequence, failed to detect I, metris. With exception 1 tool gene, PCR‐RFLP PCR sequencing evaluated this study...
ABSTRACT Due to the small number of Cryptosporidium oocysts in water, samples taken and analyses performed can affect results detection. In this study, 42 water were collected from one watershed during 20 storm events over 1 year, including duplicate or quadruplicate 16 events. Ten four had three eight subsamples. They processed by EPA method 1623, present detected immunofluorescent microscopy PCR. Altogether, 24 39 (47 67 subsamples) analyzed positive for . contrast, 36 (62 76 PCR, 10...
Three members of the Plasmodium vivax merozoite surface protein-3 (PvMSP3) family (PvMSP3-α, PvMSP3-β and PvMSP3-γ) were initially characterized later shown to be part a larger highly diverse family, encoded by cluster genes arranged head-to-tail in chromosome 10. PvMSP3-α have become genetic markers epidemiological studies, are being evaluated as vaccine candidates. This research investigates gene protein expression entire pertinent implications.A 60 kb multigene locus from 10 P. (Salvador...
BackgroundAntigenic variation by malaria parasites was first described in Plasmodium knowlesi, which infects humans and macaque monkeys, subsequently P. falciparum, the most virulent human parasite. The schizont-infected cell agglutination (SICA) variant proteins encoded SICAvar multigene family Erythrocyte Membrane Protein-1 (EMP-1) antigens var are expressed at surface of infected erythrocytes, associated with virulence, serve as determinants naturally acquired immunity. A parental...
Synthetic peptide vaccines provide the advantages of safety, stability and low cost. The success this approach is highly dependent on efficient epitope identification synthetic strategies for efficacious delivery. In malaria, Merozoite Surface Protein-9 Plasmodium vivax (PvMSP9) has been considered a vaccine candidate based evidence that specific antibodies were able to inhibit merozoite invasion recombinant proteins immunogenic in mice humans. However identities linear B-cell epitopes...
Consumption of contaminated water has been implicated as a major source Cryptosporidium infection in various outbreak investigations and case control studies. Surveys conducted regions the United States demonstrated presence oocysts 67-100% wastewaters, 24-100% surface waters, 17-26.8% drinking waters. The identity human infective potential these waterborne are not known, although it is likely that all from human-infecting species. Likewise, oocyst contamination also fully clear. Farm...
A building information model (BIM) is of upmost importance with a full life-time cycle in architecture engineering and construction industry. Smart relies on BIM to manipulate flow, data management flow. Currently, has been explored mainly for utilization, but there exist few works concerning security, e.g., audits critical models exposure sensitive models. Moreover, systems have proposed make use new computing paradigms, such as mobile cloud computing, blockchain Internet Things. In this...
With the prosperity of wireless and mobile communications, allocation resources, e.g., spectrum channels, femtocell access permissions, resource blocks D2D connections, has become a matter great concern, which leads to emergence popularity electronic auction. However, lack privacy protection fairness guarantee in existing market posed obstacles user participation auctions. Without protection, users will be worried about unauthorized exposure their commercial secrets, may hurt payoffs long...
Kra monkeys (Macaca fascicularis), a natural host of Plasmodium knowlesi, control parasitaemia caused by this parasite species and escape death without treatment. Knowledge the disease progression resilience in kra will aid effective use to study mechanisms malaria. This longitudinal aimed define clinical, physiological pathological changes infected with P. which could explain their resilient phenotype.
Cryptosporidium spp. are important causes of diarrhea in humans, ruminants, and other mammals. Comparative genomic analysis indicated that genetically related host-adapted species have different numbers subtelomeric genes encoding the Cryptosporidium-specific MEDLE family secreted proteins, which could contribute to differences host specificity. In this study, a parvum-specific member protein MEDLE-2 encoded by cgd5_4590 was cloned expressed E. coli. Immunofluorescent staining with...