The oriental fruit fly, Bactrocera dorsalis, is a destructive pest in tropical and subtropical areas. In this study, we performed transcriptome-wide analysis of the fat body B. dorsalis obtained more than 59 million sequencing reads, which were assembled into 27,787 unigenes with an average length 591 bp. Among them, 17,442 (62.8%) matched known proteins NCBI database. sequences further annotated gene ontology, cluster orthologous group terms, Kyoto encyclopedia genes genomes. depth was to...

The continuously developing pesticide resistance is a great threat to agriculture and human health. Understanding the mechanisms of insecticide key step in dealing with phenomenon. Insect cuticle recently documented delay xenobiotic penetration which breaks previous stereotype that useless resistance, while underlying mechanism remains scarce.Here, we find integument contributes over 40.0% via different delivery strategies oriental fruit fly. A negative relationship exists between thickening...

Juvenile hormone (JH) prevents metamorphosis during insect larval stages and promotes adult reproductive processes. Krüppel-homolog 1 (Kr-h1), a zinc finger transcription factor assumed to be induced by JH via the receptor methoprene-tolerant (Met), mediates antimetamorphic effect of in insects, but its function JH-mediated processes has not been fully explored. In this study, Met Kr-h1 involved signaling pathway were first cloned identified from oriental fruit fly, Bactrocera dorsalis, an...

Esterase has been reported to be involved in malathion resistance the oriental fruit fly, Bactrocera dorsalis (Hendel). However, underlying molecular mechanism of esterase-mediated remains largely unknown this species. Here, with use a strain selected for laboratory (MR), we found that two overexpressed α-esterase genes, namely BdCarE4 and BdCarE6, predominant adult midgut fat body, function conferring B. dorsalis. Notably, these genes were mostly close esterase E3, which are usually...

The oriental fruit fly Bactrocera dorsalis (Hendel), a widespread agricultural pest, has evolved resistance to many insecticides, including organophosphorus compounds. Glutathione S-transferases (GSTs) are involved in xenobiotic detoxification and insecticide insects. However, the role of delta class GSTs detoxifying malathion B. is unknown. Here, we evaluated roles two this species.Two genes, BdGSTd1 BdGSTd10, were characterized dorsalis. They highly expressed 5-day-old adults, as well...

Abstract BACKGROUND The oriental fruit fly, Bactrocera dorsalis (Hendel), is a widespread agricultural pest that has evolved resistance to many commonly used insecticides including malathion. Glutathione S ‐transferases (GSTs) are multifunctional enzymes metabolize directly or indirectly. specific mechanism by GSTs confer malathion in B. unclear. RESULTS BdGSTd9 was identified from and expressed at twice the level malathion‐resistant strain (MR) than susceptible (MS). By using RNAi of ,...

The oriental fruit fly, Bactrocera dorsalis (Hendel) is a worldwide pest damaging wide range of hosts. Due to the long-term indiscriminate use insecticides, B. has developed serious resistance several insecticides. UDP-glycosyltransferases (UGTs) are secondary metabolic enzymes involved in biotransformation and play an important role metabolism plant metabolites synthetic insecticides insects. Thus, we suspect that UGTs insecticide tolerance.In this study, 31 UGT genes were identified genome...

Fecundity is arguably one of the most important life history traits, as it closely tied to fitness. Most arthropods are recognized for their extreme reproductive capacity. For example, a single female oriental fruit fly Bactrocera dorsalis , highly invasive species that destructive agricultural pests worldwide, can lay more than 3000 eggs during its span. The ovary crucial insect reproduction and development requires further investigation at molecular level. We report here miR-309a regulator...

Abstract The citrus red mite, Panonychus citri (McGregor), is an important spider mite pest in producing areas. Owing to long-term acaricide exposure, resistance has evolved rapidly recent years. To evaluate the extent of resistance, seven field populations sampled from various geographical locations China during 2015–2018 were tested using leaf-dip bioassay method determine their susceptibilities four acaricides. In comparison with susceptible strain maintained laboratory, low or moderate...

Abstract BACKGROUND The citrus red mite, Panonychus citri (McGregor), a global pest of citrus, has developed different levels resistance to various acaricides in the field. Abamectin is one most important insecticides/acaricides worldwide, targetting wide number insect and mite pests. evolution abamectin P. threatening sustainable use for control. RESULTS resistant strain (NN‐Aba), derived from field NN by consistent selection with abamectin, showed 4279‐fold compared relatively susceptible...

Elevated esterase activities and increased band intensities of multiple isozymes after electrophoresis are commonly associated with resistance to organophosphate, pyrethroid carbamate insecticides in various heliothine spodopteran pests. One possible explanation for this involves a 'master regulator' mutation more general chemical stress response. An association between elevated isozyme has also been reported the Cry1Ac toxin Helicoverpa armigera. The basis is unclear albeit some involvement...

Polyandry Is widespread among insects but male accessory gland products can influence the propensity of former mates to copulate later with other males. In addition, females may receive nutritional supplements in fluids that substantially increase fitness regardless whether remating has been inhibited. this study, we investigated polyandry female Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) and relationship between contents. Similar fruit flies, 2 kinds glands were observed B....

Abstract BACKGROUND Long non‐coding RNAs (lncRNAs) play important roles in the regulation of biological processes and have been identified many species including insects. However, association between lncRNAs pesticide resistance insect such as Bactrocera dorsalis is unknown. RESULTS RNA‐seq was performed on malathion resistant (MR1) susceptible (MS) strains B. a total 6171 transcripts were identified. These included 3728 lincRNAs, 653 antisense lncRNAs, 1402 intronic 388 sense lncRNAs. A 40...

The oriental fruit fly, Bactrocera dorsalis, is a damaging insect pest for many vegetable and crops that has evolved severe chemical insecticide resistance, including organophosphorus, neonicotinoid, pyrethroid, macrolides. Hence, it important to elucidate its detoxification mechanism improve management mitigate resource destruction. Glutathione S-transferase (GST) critical secondary phase enzyme plays multiple functions against xenobiotics. In this study, we identified several BdGSTs by...

The citrus red mite Panonychus citri has developed strong resistance to acaricides. Cytochrome P450 monooxygenases (P450s) can detoxify pesticides and are involved in pesticide many insects. Here, a pyridaben-resistant P. strain showed cross-resistance cyenopyrafen, bifenazate, fenpyroximate, tolfenpyrad. Piperonyl butoxide, inhibitor, significantly increased the toxicity of pyridaben resistant (Pyr_Rs) susceptible (Pyr_Control) strains. activity was higher Pyr_Rs than Pyr_Control. Analyses...

The Resistance to dieldrin (Rdl) gene encodes a subunit of the insect gamma-aminobutyric acid (GABA) receptor. Cyclodiene resistance in many insects is associated with replacement single amino (alanine at position 302) either serine or glycine Rdl gene. Two Rdl-orthologous genes GABA receptors (PxGABARalpha1 and PxGABARalpha2) were cloned sequenced from susceptible strain (Roth) Plutella xylostella. PxGABARalpha1 PxGABARalpha2 showed 84% 77% identity Drosophila melanogaster an level,...

Panonychus citri is a globally dominant citrus plant pest mite. Pesticide-induced population resurgence concern for mite control. Exposure to sublethal concentrations of pesticides has stimulated reproduction and outbreak risks in many pests. Pyridaben, mitochondrial electron transport inhibitor, been frequently used worldwide In the study, transgenerational effects pyridaben exposure on Pyr_Rs (resistant) Pyr_Control (susceptible) strains were systematically investigated both exposed...

Abstract Insecticide resistance in Panonychus citri is a major obstacle to mite control citrus orchards. Pyrethroid insecticides are continually used mites China, although pyrethroids has evolved some populations. Here, the pyrethroid fenpropathrin was investigated and 7 out of 8 field‐collected populations P. exhibited high level resistance, ranging from 171‐fold 15 391‐fold higher than susceptible (SS) comparison strain. Three voltage‐gated sodium channel (VGSC) mutations were identified...