A5081262986- Reproductive Biology and Fertility
- Ovarian function and disorders
- Reproductive System and Pregnancy
- Reproductive Health and Technologies
- Sperm and Testicular Function
- Endometriosis Research and Treatment
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Pregnancy and preeclampsia studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Conducting polymers and applications
- Organic Electronics and Photovoltaics
- Circular RNAs in diseases
- Estrogen and related hormone effects
- Molecular Junctions and Nanostructures
- Ectopic Pregnancy Diagnosis and Management
- Force Microscopy Techniques and Applications
- Gestational Trophoblastic Disease Studies
- Organic Light-Emitting Diodes Research
- Biotin and Related Studies
- ATP Synthase and ATPases Research
- Acupuncture Treatment Research Studies
- Acne and Rosacea Treatments and Effects
- Virus-based gene therapy research
Second Xiangya Hospital of Central South University
2006-2023
Central South University
2008-2023
Nanjing Drum Tower Hospital
2023
First Affiliated Hospital of Xi'an Jiaotong University
2021
North China University of Science and Technology
2017
The Affiliated Yongchuan Hospital of Chongqing Medical University
2016
Chongqing Medical University
2016
Southwest University
2008
Los Alamos National Laboratory
1997
Nanjing University
1995-1996
Sensor formats have been developed for detecting the activity of proteolytic enzymes based on fluorescent conjugated polymer superquenching. These sensors employ a reactive peptide sequence within tether linking quencher to biotin. The binds containing colocated biotin-binding protein and by means biotin–biotin binding interactions, resulting in strong quenching fluorescence. Enzyme-mediated cleavage results reversal fluorescence quenching. assays protease are simple, sensitive, fast,...
An assay technology for high-throughput screening of kinase and phosphatase activities is introduced. The format based upon superquenching fluorescent-conjugated polymers by dye-labeled kinase/phosphatase peptide substrates. sensor platform composed highly polyelectrolytes colocated with the phosphate coordinating metal ion gallium on microspheres. Phosphorylated substrates containing a quencher bind specifically to ions means groups, resulting in quench polymer fluorescence. modulation...
<b><i>Background/Aims:</i></b> Abnormal apoptosis of granulosa cells (GCs) is thought to involve in the pathogenesis polycystic ovary syndrome (PCOS); however, associated cellular and molecular mechanisms remain unclear. <b><i>Methods:</i></b> Primary GCs were obtained from healthy women with PCOS. The cell proliferation analyzed insulin-stimulated insulin receptor gene (INSR) siRNA-transfected GCs. protein expression Akt-mTOR-S6K1 signal...
Introduction Asthenoteratozoospermia is one of the most common causes male infertility. Several genes have been identified as genetic causative factors, but there a considerable heterogeneity underlying asthenoteratozoospermia. In this study, we performed analysis two brothers from consanguineous Uighur family in China to identify gene mutations for asthenoteratozoospermia-related Methods Two related patients with asthenoteratozoospermia large were sequenced by whole-exome sequencing and...
Abstract Purpose To explore the key genes and molecular pathways in progression of thyroid papillary carcinoma (PTC) promoted by testosterone using RNA-sequencing technology, to provide new drug targets for improving therapeutic effect PTC. Methods Orchiectomy (ORX) was carried out construct ORX mouse models. TPC-1 cells were subcutaneously injected PTC formation mice, tumor tissues collected RNA-seq. The screened bioinformatics technology. Tnnt1 expression knocked down or overexpressed...
Increasing evidence indicates that long non-coding RNAs (lncRNAs) have been associated with cancer development. However, the contributions of lncRNAs to renal cell carcinoma (RCC) remain poorly characterized. Here, we identified a novel lncRNA, termed HEIRCC, which was up-regulated in RCC tissues through lncRNA microarray analysis and subsequent validation 60 clinical specimens lines. The high expression HEIRCC is closely pathology features such as larger tumor size, poor differentiation,...
To investigate the adverse effects of alcohol on ovarian reserve in women childbearing age.Twenty bar hostesses between ages 18 and 29 with moderate consumption for over 3 years 16 healthy 28 under a standard were recruited. Their was evaluated by measuring menstrual cycle day three (CD3) serum follicle stimulating hormone (FSH), luteinizing (LH), estradiol (E2), prolactin (PRL) testosterone (TST) levels, transvaginal B-ultrasound examination uterine size, size number antral...
Protein kinases are involved in the regulation of cellular metabolism, growth, differentiation, and proliferation. Aberrations their function can lead to diseases such as cancer inflammation. therefore possible targets for drug therapies. To address need high throughput screening potential inhibitors, QTL has developed a homogeneous robust kinase assay use multiwell plate format. The Lightspeed™ fluorescence superquenching-based assays do not require specialized equipment, nor they involve...
Objectives: To compare serum chemerin levels between women with classic hyperandrogenic PCOS, euandrogenic PCOS and matched control subjects.
Objective: To investigate the prevalence and related characteristics of oligomenorrhea among women within childbearing age in China. Study design: A large-scale community-based investigation was conducted from 2013 to 2015. total 12,964 aged 18–49 years 9 provinces/municipalities China were recruited for healthcare screening local community health centers. Outcome measures include clinical history, ultrasonographic exam, hormonal metabolic parameters. Results: Among China, 12.2%...
Using virus derived from Bombyx mori (silkworm) nuclear polyhedrosis (BmNPV), we constructed an infectious recombinant carrying the human granulocyte‐macrophage colony‐stimulating factor (hGM‐CSF) cDNA placed downstream polyhedrin promoter. The BmN cells infected with baculovirus BmNPV‐GM‐CSF expressed (r)hGM‐CSF up to 3.9 times 10(5) colony‐formation units/ml in medium. maximum activity of rhGM‐CSF was 4.3 10(6) haemolymph silkworm (Bombyx mori) larvae BmNPV‐GM‐CSF. Three distinct species...
Abstract Nitrogen permease regulator like-2 ( NPRL2 ) has been proved to be a useful suppressor gene in treating many cancers containing renal cancer based on experiments. Transgenic technology which transfect exogenous into cell was used these However, this defects, such as mutation and loss. Cytoplasmic transduction peptide (CTP) can avoid defects because it directly mediate proteins penetrate membrane specifically locate cytoplasm. In article, CTP protein the line 786-O, then...
Abstract Background Premature ovarian insufficiency (POI) plagues 1% of women under 40, while quite a few remain an unknown cause. The development sequencing has helped find pathogenic genes and reveal the relationship between DNA repair reserve. Through exome sequencing, our study targets screening out possible POI gene variants in Chinese family 20 sporadic patients, preliminarily exploring functional impact finding potential linkages POI. Results whole suggested novel FMN2 heterozygous...
To investigate the factors influencing sterility in males undergoing routine sperm inspection by masturbation.Scales for demographic data, self-compiled infertility questionnaire, Symptom Checklist-90 (SCL-90) , and sexual life subscale of Olson Marital Quality Questionnaire (ENRICH) were assessed 220 cases who had undergone examination after ejaculation.The total SCL-90 scores factor anxiety, phobia, somatization, obsessive compulsive behavior, interpersonal-sensitivity, hostility,...
To investigate the effects of repeated mifepristone and levonorgestrel use on estrous cycle expression ovarian follicle-stimulating hormone receptor (FSHR) luteinizing (LHR) in mice.Ovarian FSHR LHR mRNA was measured using real-time quantitative reverse transcription-polymerase chain reaction, while protein levels were immunohistochemistry.Repeated significantly lengthened decreased ovaries mice at 4, 24, 48 days after discontinuing drug use. Repeated had significant main length level LHR....
Objective To investigate the effect of intrauterine administration autologous peripheral blood mononuclear cells (PBMCs) on pregnancy outcomes in patients with recurrent implantation failure (RIF) and related mechanism. Methods Thirty-six RIF received PBMCs 24 h before frozen-thawed embryo transfer, observation their outcomes. Enzyme-linked immunosorbent assay (ELISA) was performed to detect concentration interleukin-6 (IL-6) tumor necrosis factor-α (TNF-α) cervical secretions after...