A5101670581- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Tea Polyphenols and Effects
- Pluripotent Stem Cells Research
- Ion Channels and Receptors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics and Neurodevelopmental Disorders
- Plant Gene Expression Analysis
- Phytochemicals and Antioxidant Activities
- MicroRNA in disease regulation
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Ginkgo biloba and Cashew Applications
- CRISPR and Genetic Engineering
- Advanced Photocatalysis Techniques
- Renal and related cancers
- Circadian rhythm and melatonin
- Ovarian function and disorders
- Genomics and Rare Diseases
- Tissue Engineering and Regenerative Medicine
- Heusler alloys: electronic and magnetic properties
- Nuclear Structure and Function
- Forest, Soil, and Plant Ecology in China
- Advanced Cellulose Research Studies
First Affiliated Hospital of GuangXi Medical University
2024
Guangxi Medical University
2024
Second Xiangya Hospital of Central South University
2021-2024
Central South University
2014-2024
Guangxi Academy of Sciences
2023-2024
Guangdong Academy of Agricultural Sciences
2017-2024
Third Xiangya Hospital
2022
Guangzhou Institutes of Biomedicine and Health
2017-2021
University of Chinese Academy of Sciences
2015-2021
Chinese Academy of Sciences
2017-2020
The human zona pellucida is composed of four glycoproteins (ZP1, ZP2, ZP3, and ZP4) has an important role in reproduction. Here we describe a form infertility with autosomal recessive mode inheritance, characterized by abnormal eggs that lack pellucida. We identified homozygous frameshift mutation ZP1 six family members. In vitro studies showed defective proteins normal ZP3 colocalized throughout the cells were not expressed at cell surface, suggesting aberrant results sequestration...
Circular RNAs (circRNAs) play an important role in cancer development and progression by regulating gene expression. The present study aimed to investigate the function of circRNA_100859 colon cancer. circRNA expression profiles from a human circRNAs chip were analyzed. effects on cell proliferation apoptosis assessed vitro interactions between its micro (mi)RNA target genes diagnostic prognostic significance was also investigated. It identified that overexpressed tissues promoted inhibited...
The ribosome-associated protein quality control (RQC) pathway acts as a translational surveillance mechanism to maintain proteostasis. In mammalian cells, the cytoplasmic RQC involves nuclear export mediator factor (NEMF)-dependent recruitment of E3 ligase Listerin ubiquitinate ribosome-stalled nascent polypeptides on lysine residue for degradation. However, nuclear-encoded mitochondrial remains elusive, these peptides can be partially imported into mitochondria through translocons,...
Abstract Background The etiology of premature ovarian insufficiency, that is, the loss activity before 40 years age, is complex. Studies suggest genetic factors are involved in 20–25% cases. aim this study was to explore oligogenic basis insufficiency. Results Whole-exome sequencing 93 patients with POI and whole-genome 465 controls were performed. In gene-burden analysis, multiple variants, including those associated DNA damage repair meiosis, more common participants insufficiency than...
Introduction Asthenoteratozoospermia is one of the most common causes male infertility. Several genes have been identified as genetic causative factors, but there a considerable heterogeneity underlying asthenoteratozoospermia. In this study, we performed analysis two brothers from consanguineous Uighur family in China to identify gene mutations for asthenoteratozoospermia-related Methods Two related patients with asthenoteratozoospermia large were sequenced by whole-exome sequencing and...
Summary In the present study, we employed high performance liquid chromatography with an amide‐C 16 column to determine eighteen major active ingredients in black tea, including theanine, gallic acid, four purine alkaloids, eight catechins and theaflavins. The method was successfully used analyse two new kinds of teas from leaves Camellia ptilophylla kucha China several other world‐ famous teas. Forty percentage ethanol chosen as extraction solvent for preparing tea extracts. All compounds...
A recently discovered tea [Camellia sinensis (L.) O. Kuntze] cultivar can generate tender shoots in winter. We performed comparative proteomics to analyze the differentially accumulated proteins between winter and spring of this clonal reveal physiological basis its evergrowing character during extracted from (newly formed two leaves a bud) "Dongcha11" respectively. Thirty-three high-confidence were identified by matrix-assisted laser desorption ionization time flight mass spectrometry...
Abstract Objective To develop an ultrasound-driven clinical deep learning radiomics (CDLR) model for stratifying the risk of testicular masses, aiming to guide individualized treatment and minimize unnecessary procedures. Methods We retrospectively analyzed 275 patients with confirmed lesions (January 2018 April 2023) from two hospitals, split into training (158 cases), validation (68 external test cohorts (49 cases). Radiomics (DL) features were extracted preoperative ultrasound images....
The β phase stability in poly(vinylidene fluoride/trifluoroethylene) [P(VDF-TrFE)] thin films was studied below 300 K using X-ray diffraction and polarization-electric-field (P-E) hysteresis loops measurements. On as-grown samples, an irreversible partial order-disorder transformation at Tβ ∼ 250 K, namely, the relaxation temperature, evidenced by appearance of additional X-Ray peak above as well changes on P-E heating after first cooling. This order-disorder-like which is attributed to...
Zona pellucida (ZP), which enwraps the oocyte during folliculogenesis, initially forms in primary follicle and plays an important role female fertility. Here, we investigated a mouse strain ("mutant mice" for short) carrying two types of ZP defects i.e., thinned (but intact) cracked, caused by targeted mutation Zp1 gene. Using this mutant wild-type as control, studied effects on development oocytes granulosa cells folliculogenesis. For each defect, examined morphology transzonal projections...
Abstract Tea (Camellia sinensis [L.] O. Kuntze) tree is a perennial plant in which winter dormancy an important biological adaptation to environmental changes. We discovered and reported novel tea cultivar that can generate tender shoots several years ago, but the molecular mechanism for this unique phenotype remains unknown . Here, we conducted comparative transcriptomics, proteomics metabolomics along with phytohormone quantitation between spring investigate physiological basis putative...
Platelet-derived growth factor BB (PDGF-BB) is a potential biomarker of tumor angiogenesis. For the first time, we developed highly sensitive aptasensor for PDGF-BB with an enhanced test line signal by using two different gold nanoparticles (AuNPs). Herein, describe biosensor detection that combines biotinylated aptamer on sample pad and poly thymine-Cy3-AuNP-monoclonal antibody complexes against immobilized conjugate A. Streptavidin (SA) rabbit anti-mouse polyclonal were also in...
Transient receptor potential ankyrin 1 (TRPA1), the non-selective cation channel, was found that can mediate generation of multiple sclerosis, while mechanism is still controversial. Lysophosphatidylcholine (LPC) a critical trigger sclerosis which results from syndrome neuronal inflammation and demyelination. In this work, we suggested TRPA1 LPC-induced oxidative stress cytotoxicity in OLN-93 oligodendrocyte. The expression detected by using quantitative real-time PCR (qRT-PCR)...
Abstract Introduction Decision making as a complex cognitive process involves assessing risk, reward, and costs. Typically, it has been studied in nonsocial contexts. We have developed novel laboratory model used with rodents to detect food‐foraging decision‐making strategies different social settings. However, the brain regions that mediate these behaviors are not well identified. Substantial evidence shows anterior cingulate cortex ( ACC ) participates evaluation of information decision...
Zona pellucida (ZP), which is composed of at most four extracellular glycoproteins (ZP1, ZP2, ZP3, and ZP4) in mammals, shelters the oocytes vital female fertility. Several studies have identified indispensable roles ZP1-3 maintaining normal However, understanding ZP4 still very poor because only one study on ZP4-associated infertility performed rabbits has been reported up to date. Here we investigated function mammalian Zp4 by creating a knockout (KO) rat strain (Zp4-/- rat) using...
The zona pellucida (ZP) plays vital roles in reproductive processes including oogenesis, fertilization, and preimplantation development. Both human rat ZP consist of four glycoproteins, called ZP1, ZP2, ZP3, ZP4. Our previous research reported a novel Zp1 mutation cases infertility, associated with an abnormal phenotype involving the absence ZP. Here, we developed homologous strain to investigate pathogenic effect. ovaries homozygous (Zp1MT/MT) females possessed both growing fully grown...