A5101796490- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- RNA Research and Splicing
- Hernia repair and management
- Cellular Mechanics and Interactions
- Gastrointestinal disorders and treatments
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Advanced materials and composites
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Cancer Mechanisms and Therapy
- Protein Structure and Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- High-Temperature Coating Behaviors
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Ovarian cancer diagnosis and treatment
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- High Entropy Alloys Studies
Fujian Medical University
2019-2023
Fujian Provincial Hospital
2019-2020
Shanghai East Hospital
2020
Wuhan University
2014-2017
Wuhan Institute of Bioengineering
2014-2017
In-Q-Tel
2017
Abstract This study aimed to identify differential circular RNA (circRNA) in the plasma exosomes of patients with lung adenocarcinoma (LUAD) using high‐throughput sequencing. First, were isolated an exosome isolation kit and confirmed by Western blotting, transmission electron microscopy, NanoSight Assay. Subsequently, circRNA expression profiles screened sequencing fluorescence quantitative real‐time polymerase chain reaction (qRT‐PCR) Sanger Finally, circRNA‐miRNA‐mRNA network was...
Understanding the microtubule-dependent behaviors of viruses in live cells is very meaningful for revealing mechanisms virus infection and endocytosis. Herein, we used a quantum dots-based single-particle tracking technique to dynamically globally visualize transport influenza cells. We found that intersection configuration microtubules can interfere with cells, which lead changing long-time pausing behavior viruses. Our results revealed most moved along straight rapidly unidirectionally...
Abstract Exosomal microRNAs (miRNAs) have great potentials as a novel biomarker to predict lung cancer. We applied miRNA microarray identify aberrantly expressed serum exosomal miRNAs candidate biomarkers for patients with adenocarcinoma (LUAD). Compared the normal control, 31 were found be upregulated and 29 downregulated in of LUAD respectively. Then, 10 dysregulated expression levels further validated via qRT‐polymerase chain reaction. Notably, miR‐7977 was highest miR‐98‐3p lowest LUAD,...
Abstract Background Growing evidence has indicated the vital parts of long non-coding RNAs (lncRNAs) in modulating progression assorted human cancers, including cervical cancer (CC). Nevertheless, role and mechanism aspartyl-tRNA synthetase antisense RNA 1 (DARS-AS1) have been not comprehensively illustrated CC yet. Methods Real-time quantitative polymerase chain reaction (RT-qPCR) was exploited for assessing expression while western blot protein cells. The cell counting kit-8 (CCK-8),...
Ovarian cancer (OvCa) is the most lethal gynaecological malignancy worldwide. We aimed to illustrate potential function and molecular mechanism of exosomal microRNA-543 (miR-543) in oncogenesis development OvCa. Differentially expressed microRNAs exosomes derived from OvCa cell lines were identified by bioinformatic analysis verified RT-PCR. Cell proliferation ability was estimated clonogenic 5-ethynyl-2'-deoxyuridine assays vitro vivo. Potential involved pathways targets miRNAs analysed...
Long non-coding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) has been predicted to play a critical role in various biological processes including tumorigenesis. However, the clinical significance of UCA1 lung adenocarcinoma (LUAD) is still not understood detail. The aim this study was assess expression levels LUAD based on publicly available data and evaluate its potential signaling pathways.The RNA-sequencing (RNA-seq) dataset information all patients were downloaded from Cancer...
Background Lung cancer is the most prevalent and deadliest worldwide. The present study aims to determine prognosis value of low expression long non-coding RNAs (lncRNAs) in LUAD. Methods RNA-seq data clinical information were downloaded from Cancer Genome Atlas (TCGA) data-base. Dysregulated genes between LUAD paracancerous tissue screened by GeneSpringGX. Prognostic lncRNAs which expressed filtrated Ualcan, then further verified through TCGA database. association clinicopathological...
An integrated mcirofluidic-based cell migration platform was developed to explore the vaccinia virus-induced in different shear stress environments.
To establish the utility of an ultrasonographic scoring system for diagnosis adnexal torsion.We retrospectively analyzing 358 torsion cases. Using Pearson's χ2 test we determined whether signs were significantly associated with torsion. Receiver operating characteristic curves used to evaluate diagnostic efficacy system. Ultimately by using binary logistic regression established a precise and convenient system.The score was based on five criteria that identified be independently torsion: (1)...
Translation is one of the many critical cellular activities regulated by viruses following host-cell invasion, and studies viral mRNA translation kinetics subcellular localization require techniques for dynamic, real-time visualization translation. However, conventional tools imaging often coding region modifications that may affect native Here, we achieve dynamic with a tool labels target mRNAs unmodified regions using CRISPR/dCas13 system specific complementary paired guide RNAs. This...